Fig 1: (A) Ferrous iron (Fe2+) contents and transferrin receptor (TFRC) levels, (B) glutathione peroxidase 4 (GPX4) activities, reduced glutathione (GSH), and 4-hydroxynonenal (4-HNE) levels in lungs of mice at two time points after cecal ligation and puncture (CLP). Calcitriol treatment reduced Fe2+, TFRC, and 4-HNE levels, and enhanced GSH levels and GPX4 activities in ovariectomized mice with sepsis. OB, mice with a sham-ovariectomy operation and a high-fat diet (HFD) (n = 8); OVSS, mice with an ovariectomy, an HFD, and then cecal ligation and puncture (CLP) was performed, and saline was injected after CLP, sacrificed on either 24 h (OVSS-24) or 72 h (OVSS-72) (n = 8 at each time point); and OVSD, mice with an ovariectomy, an HFD, and CLP, and calcitriol was injected after CLP, sacrificed on either 24 h (OVSD-24) or 72 h (OVSD-72) (n = 8 at each time point). Values are expressed as the mean ± SEM. Comparisons among experimental groups were analyzed by a two-way ANOVA followed by the Bonferroni post hoc test. +Significantly differs from the OVSS group on 72 h. ⁣∗Significantly differs from the OVSS group at the same time point (p < 0.05).