Description
The ViraPower™ II Lentiviral Gateway® Expression System includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s ViraPower™ II Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.2⁄V5-DEST™ vector in the kit has the CMV promoter for driving constitutive expression of the target gene in mammalian cells. The ViraPower™ II Lentiviral system uses the PGK promoter instead of SV40 promoter in the lentiviral vector for driving long-term, persistent expression of the blasticidin stable selection marker.
Advantages
• Generates replication-incompetent lentivirus for transducing dividing and non-dividing mammalian cells
• Easy recombination-based cloning using Gateway® technology
• Stable expression, long term expression
Key Features
• CMV promoter for high level, constitutive expression of the target gene
• Blasticidin selection marker for stable selection under control of PGK promoter for long-term, persistent expression
Kit includes
• pLenti6.2⁄V5-DEST™ Gateway® Vector Kit (Cat # V36820)
• ViraPower™ Bsd Lentiviral Support Kit (Cat # K497000)
• 293FT Cell Line (Cat # R70007)
For research use only. Not intended for any therapeutic or diagnostic use