Description
For researchers studying G protein-coupled receptors (GPCRs) and other membrane proteins, the Expi293™ MembranePro™ Expression System delivers enriched, functional membrane proteins with high efficiency, scalability and reliability for a wide variety of low or high throughput applications.
Features of the Expi293™ MembranePro™ Expression System include:
• Efficient—more than 20-fold increase in membrane protein yield compared to the adherent culture system
• Scalable—easy scale-up without the need for multiple flasks
• Easy to use—no enzymatic or mechanical dissociation required
In addition, benefits of MembranePro™ technology include:
• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian post-translational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors
With the less labor-intensive Expi293™ MembranePro™ Expression System protocol, you will:
1. Clone your gene of interest using a TOPO® vector
2. Transfect a susupension culture of Expi293F™ cells using Expifectamine™ 293 transfection reagent
3. Harvest lipoparticles 48 hrs after addition of enhancers using MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays
Note: Exi293F™ Cells (A14527) and a pEF6 V5-His TOPO® Expression Kit (K9610-20) are required for use of this system and must be purchased separately when needed