Precision gRNA Synthesis Kit A29377 from Thermo Fisher Scientific

Product Specs
ItemPrecision gRNA Synthesis Kit
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberA29377
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Quantity25 reactions
ApplicationsPCR

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Thermo Fisher Scientific

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Waltham, MA 02451

United States

Phone: 800-678-5599

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Website: www.thermofisher.com

(1) Cryopreservation of human cortical organoids using vitrificationFront Mol NeurosciSeptember 8, 2026Kyle Stokes, Sandra Mojica-Perez, Samantha Jacobs, Joy Huang, Shivanshi Vaid, Yukun Yuan, Caroline A. Pearson, Daniel Montes, Andrew Tidball, Debora VanHeyningen, et al.with a heterozygous mutation in STXPB1 generated by using the Precision gRNA synthesis kit (Thermo, #A29377) and transfected with TrueCut™ Cas9 protein v2 (Thermo, #A36496) using the Neon™ transfection electroporation

(2) De novo structural variants in autism spectrum disorder disrupt distal regulatory interactions of neuronal genesGenome ResearchSeptember 1, 2026Ketrin Gjoni, Xingjie Ren, Amanda Everitt, Yin Shen, Katherine S. Pollard(Supplemental Table S2) were in vitro transcribed using the precision gRNA synthesis kit (Invitrogen A29377), and Cas9-NLS protein was ordered from QB3 MacroLab at the University of California, Berkeley. We assembled

(3) Phospholipid flippases ATP8A1 and ATP8A2 regulate GABAA receptor trafficking through distinct mechanisms in hippocampal neuronsiScienceJuly 17, 2026Muneyuki Kawase, Shu Tokunaga, Yuta Umemura, Hossam H. Shawki, Jumpei Omi, Ayana Hoshiai, Tomoha Ohshima, Tsuzumi Nakajima, Takao Kohno, Takashi Sakurai, et al.commercial assays,Critical commercial assays ,, Precision gRNA Synthesis Kit,Thermo Fisher Scientific,Cat#A29377 Protein Assay BCA Kit,Fujifilm Wako Pure Chemical,Cat#297-73101 ISOSPIN Cell & Tissue RNA,Nippon Gene,Cat#314-08211 ,, Deposited

(4) A de novo LDLR mutation in severe familial hypercholesterolemia: case report, functional characterization, and a personalized gene correction strategy explorationFront Cardiovasc MedJune 9, 2026Shuran Zhang, Wenming Huang, Haoqiang Chen, Ninghui Mu, Le Chang, Baosheng Zhu, Jinman Zhang, Ying Chan(tracrRNA portion), the whole sequences of sgRNA were synthesized by GeneArt Precision gRNA Synthesis kit (A29377, Invitrogen™, ThermoFisher). Design and synthesis of single strand donor DNA: The genomic template sequence

(5) Electroporation-mediated site-specific knock-in of a human coding sequence into the bovine ALB locus in embryosBMC BiotechnologyJune 6, 2026Kyeong-Hyeon Eom, Soo-Young Yum, Gyeong-Min Gim, Woo Jae Son, Young-Chai Kim, Jae-Yoon Choi, Dong-Hyeok Kwon, Goo Janginstructions, sgRNAs were synthesized using the Precision gRNA Synthesis Kit (Thermo Fisher Scientific, A29377). Residual DNA were removed using DNase I. Purified sgRNA was eluted in nuclease-free water and quantified

(6) Cellular context and ligand class shape CXCR4-CCR5 heteromerization in live cellsResearch SquareMay 20, 2026Mohamed Seghiri, Mohamed Seghiri, Mojeed Farshadfar, Elizabeth Hair, Andres Ruiz, Ceyvion Wilkins, Adam W. Farshadfarthe Precision gRNA Synthesis Kit (Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA; cat. no. A29377) from PCR-amplified DNA templates and purified with the MEGAclear kit (Invitrogen, Thermo Fisher Scientific,

(7) Stage‐Specific H3K14 and H3K23 Succinylation Orchestrates Insect Metamorphosis and OogenesisAdvanced ScienceMay 10, 2026Yu‐Pu Jing, Lunjie Li, Libin Yang, Lin‐Jie Zhang, Qiang Yan, Ci Zhang, Zanrong Wen, Guangze Yu, Qian Li, Shutang Zhoupad. Single‐guide RNAs (sgRNAs) were synthesized using the Precision gRNA Synthesis Kit (Invitrogen, A29377). Two 99‐nt single‐stranded oligodeoxynucleotide (ssODN) donors with the targeted lysine substituted

(8) Improvement of chicken genome editing efficiency in vitro using ribonucleoprotein-mediated CRISPR/Cas9 deliveryJ Anim Sci BiotechnolMay 4, 2026Yujin Han, Chan Young Kwon, Jae Yong Hantargeting DAZL, CVH, and STRA8 were in vitro transcribed (IVT) using the Precision gRNA Synthesis kit (#A29377, Thermo Fisher) according to the manufacturer’s protocol with the corresponding oligonucleotides (Table

(9) CRISPR tiling deletion screens reveal functional enhancers and allelic compensation effects (ACE) on SIN3A transcriptionNature CommunicationsMarch 25, 2026Xingjie Ren, Lina Zheng, Yuxi Liu, Lenka Maliskova, Tsz Wai Tam, Yifan Sun, Hongjiang Liu, Xiekui Cui, Jerry Lee, Maya Asami Takagi, et al.Sanger sequencing. We in vitro transcribed all sgRNA using the Precision gRNA Synthesis Kit (Invitrogen, A29377), and obtained Cas9-NLS protein from QB3 MacroLab at the University of California, Berkeley. We delivered

(10) Ectopic expression of cytosolic DHODH uncouples de novo pyrimidine biosynthesis from mitochondrial electron transportNature MetabolismFebruary 17, 2026Andrea Curtabbi, Sara Natalia Jaroszewicz, Rocío Sanz-Cortés, Rebeca Acín-Pérez, Dimitrios Prymidis, Maksym Cherevatenko, Raquel Martínez-de-Mena, María Jesús Esteban-Amo, Miguel A. de la Fuente, Christian Frezza, et al.(http://crispor.tefor.net), and sgRNAs were synthesized using the GeneArt Precision gRNA Synthesis Kit (Invitrogen, A29377). Nucleofection was carried out at 1230 V or 1300 V (four pulses of 10 ms) for U2OS or 143B cells, respectively.

(11) Optimal pairing of binder and co-stimulatory domains improves dual CART cell efficacyMolecular therapy : the journal of the American Society of Gene TherapyNovember 5, 2025Shukla D, Manne S, Jiang S, Ruella M, Wherry EJ, Riley JL.synthesized via in vitro transcription using GeneArt Precision gRNA Synthesis Kit (Invitrogen; Cat# A29377,). Nalm6 cells were electroporated using the 4D-Nucleofector Core/X Unit (Lonza; Cat# AAF-1002B and

(12) Effect of gamma-ray exposure on the genome-editing efficiency of improved genome-editing via oviductal nucleic acids delivery (i-GONAD)Experimental AnimalsOctober 8, 2025Anarkhuu Bold-Erdene, Kento Miura, Norimasa Yamasaki, Shuka Miura, Sawako Ogata, Megumi Sasatani, Takashi Yamamoto, Osamu Kaminumausing CRISPOR (http://crispor.tefor.net) and produced using a GeneArt Precision gRNA Synthesis Kit (#A29377; Thermo Fisher Scientific). The sgRNAs for Hr mutation were designed to recognize the target site of

(13) A Novel Early Memory-Enriched Allogeneic NKG2D CAR-T Cell Therapy Based on CRISPR/Cas9 Technology for Solid TumorsCancers (Basel)September 30, 2025Cristina Aparicio, Mónica Queipo, Marina Belver, Francisco Espeso, Julia Serna-Pérez, Lucía Enríquez-Rodríguez, Carlos Acebal, Álvaro Martín-Muñoz, Antonio Valeri, Alejandra Leivas, et al.Enrichment Synthesis and purification of guide RNAs (gRNAs) was performed with GeneArt™ Precision gRNA Synthesis Kit (A29377, Invitrogen) according to the manufacturer’s instructions. gRNAs targeting constant regions of TCRα

(14) Requirements for establishment and epigenetic stability of mammalian heterochromatinMolecular CellSeptember 18, 2025Antonis Tatarakis, Harleen Saini, Juntao Yu, Wenzhi Feng, Carlos A. Pinzon-Arteaga, Danesh Moazed(5′-TGTGAATCGTAGGAGCGCTG- 3′) was synthesized via in vitro GeneArt™ Precision gRNA Synthesis Kit (ThermoFisher, A29377) following manufacturer’s instructions. SpCas9 protein was purified by the Initiative for Genome Editing

(15) PAM-flexible adenine base editing rescues hearing loss in a humanized MPZL2 mouse model harboring an East Asian founder mutationNat CommunAugust 5, 2025Shao Wei Hu, Sohyang Jeong, Luoying Jiang, Hansol Koo, Zijing Wang, Won Hoon Choi, Biyun Zhu, Heeyoung Seok, Yi Zhou, Min Gu Kim, et al.mixture and microinjection sgRNA was synthesized using the GeneArt Precision sgRNA Synthesis Kit (A29377, Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. Microinjection

(16) Spindle checkpoint can secure additional cheating time for selfish expanded centromeresCurrent BiologyAugust 4, 2025R. Zaak Walton, Sebastian J. Khan, Warif El El Yakoubi, Takashi Akera5’-ACACTGAAAAACACATTCGT-3’) synthesized using GeneArt Precision gRNA Synthesis kit (ThermoFisher, Cat# A29377) in the reaction buffer (2 mM HEPES, 10 mM NaCl, 5 mM MgCl2, 10 μM EDTA, pH 6.5) and incubating at room

(17) CRISPR/Cas9-Mediated Knockout of the Corazonin Gene Indicates Its Regulation on the Cuticle Development of Desert Locusts (Schistocerca gregaria)InsectsJuly 9, 2025Yingying He, Qiang Yan, Yong Bi, Guosheng Liu, Shuang Hou, Xinyi Chen, Xiaoming Zhao, Xueyao Zhang, Min Zhang, Jianzhen Zhang, et al.In Vitro The sgRNA was synthesized according to the protocol provided in the Precision gRNA Synthesis Kit (A29377, Invitrogen, Waltham, MA, USA). The genomic fragment containing the target site was amplified with the

(18) EndoMAP.v1 charts the structural landscape of human early endosome complexesNatureJuly 1, 2025Gonzalez-Lozano MA, Schmid EW, Miguel Whelan E, Jiang Y, Paulo JA, Walter JC, Harper JW.(300 cycles; Illumina, MS-103-1001); GeneArt Precision gRNA synthesis kit (Thermo Fisher Scientific, A29377); RNAeasy Qiagen kit (Qiagen, 74104); 24-well glass-bottom plates (Cellvis, P24-1.5H-N); Corning square

(19) Single-cut gene therapy in a one-step generated rhesus monkey model of Duchenne muscular dystrophyCell Reports MedicineApril 15, 2025Raoxian Bai, Wenting Guo, Ting Zhang, Shuaiwei Ren, Jie Liu, Puhao Xiao, Junyu Zhang, Wenjie Sun, Jiao Yang, Yue Ma, et al.mMESSAGE mMACHINE® T7 Ultra Kit,Invitrogen,Cat#AM1345 GeneArtTM Precision gRNA Synthesis Kit,Invitrogen,Cat#A29377 Lipofectamine™ 3000 Transfection Reagent,Invitrogen,Cat#L3000008 Guide-it sgRNA Screening Kit,Takara,Cat#632639 PrimeScript™

(20) GAF-dependent chromatin plasticity determines promoter usage to mediate locust gregarious behaviorThe EMBO JournalApril 7, 2025Xiao Li, Feng Jiang, Qing Liu, Zizheng Zhang, Wenjing Fang, Yutong Wang, Hongran Liu, Le Kangtargeting the henna DHS1 region was synthesized using the GeneArt Precision gRNA Synthesis Kit (Invitrogen, A29377). The assembled DNA templates, containing the T7 promoter and the gRNA sequence, were subjected to in vitro transcription

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Precision gRNA Synthesis Kit from Thermo Fisher Scientific

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Precision gRNA Synthesis Kit from Thermo Fisher Scientific

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