Description
The ViraPower™ HiPerform™ Lentiviral Gateway® Expression Kit includes all the components needed to generate lentivirus, including vector kit, 293FT cell line, and the support kit. This kit combines Invitrogen’s™ ViraPower™ HiPerform™ Lentiviral and Gateway® technologies to facilitate easy recombination-based cloning and lentiviral-based, high-level expression of a target gene in dividing and non-dividing mammalian cells. The pLenti6.3⁄V5-DEST™ vector is equipped with two key genetic elements, making it a HiPerform™ vector: the Woodchuck Posttranscriptional Regulatory Element (WPRE) and the central Polypurine Tract (cPPT) sequence from the HIV-1 integrase gene to produce at least 4-fold increase in protein expression compared to vectors lacking these elements.
Advantages
• Stable expression
• Long-term experiments
• Accurate titer of functional virus
• Flexible and versatile Gateway® recombination cloning technology
Key Features
• HiPerform™ WPRE and cPPT elements
• CMV promoter
• V5 epitope tag at C terminus
• Blasticidin selection
Kit includes
• pLenti6.3⁄V5-DEST™ Gateway® Vector Kit (V533-06)
• ViraPower™ Bsd Lentiviral Support Kit (K4970-00)
• 293FT Cell Line (R700-07)
For research use only. Not intended for any therapeutic or diagnostic use