pLenti6.3/V5-DEST Gateway Vector Kit V53306 from Thermo Fisher Scientific

Product Specs
ItempLenti6.3/V5-DEST Gateway Vector Kit
CompanyThermo Fisher Scientific
Price Supplier Page View Company Product Page
Catalog NumberV53306
Regulatory StatusFor Research Use Only. Not for use in diagnostic procedures
Quantity1 kit
ApplicationsGene Expression

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Thermo Fisher Scientific

168 Third Avenue

Waltham, MA 02451

United States

Phone: 800-678-5599

Company Profile

Website: www.thermofisher.com

(1) Fast label-free live imaging with FlowVision reveals key principles of cancer cell arrest on endothelial monolayersThe EMBO JournalJanuary 6, 2026Gautier Follain, Sujan Ghimire, Joanna W Pylvänäinen, Monika Vaitkevičiūtė, Iván Hidalgo-Cenalmor, Diana Wurzinger, Camilo Guzmán, James R W Conway, Michal Dibus, Jouni Härkönen, et al.manufacturer’s instructions in the pLenti6.3/TO/V5-DEST destination vector (ThermoFisher Scientific, V53306). The 4 plasmids, pENTR2b-Lifeact-mNeonGreen, pENTR2b-Lifeact-mScarlet-I, pLenti6.3/TO/V5-DEST-Lifeact-mNeonGreen,

(2) Supraphysiological Androgens Promote the Tumor Suppressive Activity of the Androgen Receptor through cMYC Repression and Recruitment of the DREAM ComplexCancer researchSeptember 1, 2023Nyquist MD, Coleman IM, Lucas JM, Li D, Hanratty B, Meade H, Mostaghel EA, Plymate SR, Corey E, Haffner MC, et al.achieved using a custom vector made by replacing the promoter of pLenti6.3 (Thermo Fisher Scientific, no. V53306) with EF1α and cloning in the MYC or RBL2 ORF from a custom entry vector. RB1 overexpression was achieved

(3) PIMT regulates hepatic gluconeogenesis in miceiScienceMarch 17, 2023Kapadia B, Behera S, Kumar ST, Shah T, Edwin RK, Babu PP, Chakrabarti P, Parsa KVL, Misra P.et al. 201831 pLenti-PIMT (wt and mutants),GW cloning,Thermo Fisher (pLenti 6.3 kit)Catalog number: V53306 pLenti-PIMT-3'UTR,This study,Human 293T cDNA was used as the template pGL3-PEPCK promoter,In lab cloning,Kapadia

(4) Bi-allelic ATG4D variants are associated with a neurodevelopmental disorder characterized by speech and motor impairmentNPJ genomic medicineFebruary 10, 2023Morimoto M, Bhambhani V, Gazzaz N, Davids M, Sathiyaseelan P, Macnamara EF, Lange J, Lehman A, Zerfas PM, Murphy JL, et al.Scientific, Carlsbad, CA) to recombine the ATG4D ORF into the pLenti6.3/V5-DEST destination vector (V53306, Invitrogen/Thermo Fisher Scientific, Carlsbad, CA). Single clones were Sanger sequenced to verify the

(5) Novel Calcium-Binding Ablating Mutations Induce Constitutive RET Activity and Drive TumorigenesisCancer ResearchOctober 17, 2022Junya Tabata, Takashi Nakaoku, Mitsugu Araki, Ryunosuke Yoshino, Shinji Kohsaka, Ayaka Otsuka, Masachika Ikegami, Ayako Ui, Shin-ichiro Kanno, Keiko Miyoshi, et al.GeneArt, a part of Thermo Fisher Scientific, and ligated into pLenti6.3/V5-DEST plasmids (catalog no. V53306, RRID:Addgene_56658; Invitrogen). The final constructs were verified by Sanger sequencing. Expression

(6) Transplantation of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells Promotes Forelimb Functional Recovery after Cervical Spinal Cord InjuryCellsSeptember 5, 2022Zheng Y, Gallegos CM, Xue H, Li S, Kim DH, Zhou H, Xia X, Liu Y, Cao Q.EGFP was constructed using pLenti6.3/V5-DEST (Thermo Fisher Scientific, Waltham, MA, USA, Cat. No. V53306) as the backbone, with the EGFP expression cassette driven by a constitutively on CMV promoter. Lentiviruses

(7) Detection and Validation of Circular DNA Fragments Using Nanopore SequencingFrontiers in GeneticsMay 30, 2022Alicia Isabell Tüns, Till Hartmann, Simon Magin, Rocío Chamorro González, Anton George Henssen, Sven Rahmann, Alexander Schramm, Johannes Kösterplasmids, pDONR223_MYC_WT (RRID:Addgene82927) and ALK_pLenti (recombined plasmid of pLenti6.3/V5-DEST™, V53306, Thermo Fisher Scientific and pDONR223-ALK, RRID:Addgene23917) were mixed in the same ratio and amplified

(8) Discovery of small-molecule positive allosteric modulators of Parkin E3 ligaseiScienceJanuary 21, 2022Shlevkov E, Murugan P, Montagna D, Stefan E, Hadzipasic A, Harvey JS, Kumar PR, Entova S, Bansal N, Bickford S, et al.,Thermo Fisher Scientific,Cat#: L3000008 pLenti6.3/V5-DEST Gateway cloning kit,Thermo Fisher Scientific,Cat#: V53306 pLenti4/V5-DEST Gateway cloning kit,Thermo Fisher Scientific,Cat#: K498000 QuikChange LightningTM

(9) WASp triggers mechanosensitive actin patches to facilitate immune cell migration in dense tissuesDevelopmental cellJanuary 10, 2022Gaertner F, Reis-Rodrigues P, de Vries I, Hons M, Aguilera J, Riedl M, Leithner A, Tasciyan S, Kopf A, Merrin J, et al.al., 2005)",N/A Plasmid: LifeAct-mCherry,"(Riedl et al., 2008)",N/A Plasmid: pLenti6.3,Invitrogen,#V53306 Plasmid: pCMV-dR8.91,Creative Biogene,#OVT2971 Plasmid: pCMV-VSV-G,"(Stewart et al., 2003)",RRID:

(10) Unearthing novel fusions as therapeutic targets in solid tumors using targeted RNA sequencingFrontiers in oncologyJanuary 1, 2022An S, Koh HH, Chang ES, Choi J, Song JY, Lee MS, Choi YL.18. Cloning was performed by subcloning two fragments into the pLenti6.3-V5-DEST vector (Invitrogen, V53306) using the sequence-and ligation-independent cloning method. The 293FT cell line from ATCC was transfected

(11) PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinomaNature communicationsJuly 30, 2021Giuliani V, Miller MA, Liu CY, Hartono SR, Class CA, Bristow CA, Suzuki E, Sanz LA, Gao G, Gay JP, et al.cDNA sequences were integrated into the lentiviral expression vector pLenti6.3/V5-Dest (Invitrogen #V53306) by an LR Gateway reaction. For viral transfections, the lentivirus packaging vectors used were psPAX2

(12) Buckling of an Epithelium Growing under Spherical ConfinementDevelopmental cellSeptember 14, 2020Trushko A, Di Meglio I, Merzouki A, Blanch-Mercader C, Abuhattum S, Guck J, Alessandri K, Nassoy P, Kruse K, Chopard B, et al.Commercial Assays,Critical Commercial Assays ,, pLenti6.3/V5-DEST™Gateway™ Vector Kit,Invitrogen,Cat#V53306 ,, Deposited Data,Deposited Data,Deposited Data ,, All Imaging raw data,Roux Lab,Trushko -> Data Raw

(13) STAT3 modulates reprogramming efficiency of human somatic cells; insights from autosomal dominant Hyper IgE syndrome caused by STAT3 mutationsBiology openSeptember 10, 2020Yu Z, Dmitrieva NI, Walts AD, Jin H, Liu Y, Ping X, Ferrante EA, Qiu L, Holland SM, Freeman AF, et al.Invitrogen's Gateway Cloning System, the STAT3 cDNA was subcloned to pLenti6.3⁄V5-DEST (Invitrogen, V53306). The virus was produced in HEK293FT cells using the ViraPower™ HiPerform™ Lentiviral Gateway Expression Kit

(14) Combined TP53 and RB1 Loss Promotes Prostate Cancer Resistance to a Spectrum of Therapeutics and Confers Vulnerability to Replication StressCell reportsMay 26, 2020Nyquist MD, Corella A, Coleman I, De Sarkar N, Kaipainen A, Ha G, Gulati R, Ang L, Chatterjee P, Lucas J, et al.Table S4,,N/A Recombinant DNA,Recombinant DNA,Recombinant DNA pLenti6.3/V5-DEST,ThermoFisher,Cat# V53306 pLentiCRISPRv2,Addgene,Cat# 52961 CloneJET PCR Cloning Kit,ThermoFisher,Cat# K1232 Software and Algorithms,Software

(15) Molecular changes in solitary fibrous tumor progressionJournal of molecular medicine (Berlin, Germany)October 1, 2019Park HK, Yu DB, Sung M, Oh E, Kim M, Song JY, Lee MS, Jung K, Noh KW, An S, et al.gateway entry vector pCR8/GW/Topo (Invitrogen, K250020) and then subcloned into pLenti6.3/V5-DEST (Invitrogen, V53306). Full-length sequences of NAB2-STAT6 fusion variants were validated by Sanger sequencing. Transient transfection

(16) Perturbed myoepithelial cell differentiation in BRCA mutation carriers and in ductal carcinoma in situNature communicationsSeptember 13, 2019Ding L, Su Y, Fassl A, Hinohara K, Qiu X, Harper NW, Huh SJ, Bloushtain-Qimron N, Jovanović B, Ekram M, et al.Addgene75. cDNA was inserted into pLenti6.3 Gateway compatible lentiviral vector (Life Technologies, cat#V53306). To produce lentiviral supernatants, HEK293T cells were co-transfected with shRNA or expression vectors,

(17) Mitochondrial One-Carbon Pathway Supports Cytosolic Folate Integrity in Cancer CellsCellNovember 29, 2018Zheng Y, Lin TY, Lee G, Paddock MN, Momb J, Cheng Z, Li Q, Fei DL, Stein BD, Ramsamooj S, et al.DNA ,, Gateway-pDEST17,Invitrogen,Cat#11803012 Gateway-pDEST10,Invitrogen,Cat#11806–015 Gateway-plenti6.3/V5-DEST,Invitrogen,Cat#V53306 pLEX_305,David Root Lab (Unpublished),Addgene Plasmid#41390 PX459_V2.0,"(Ran et al., 2013)",Addgene

(18) Remodeling of the Acetylproteome by SIRT3 Manipulation Fails to Affect Insulin Secretion or β Cell Metabolism in the Absence of OvernutritionCell reportsJuly 3, 2018Peterson BS, Campbell JE, Ilkayeva O, Grimsrud PA, Hirschey MD, Newgard CB.used according to manufacturer’s instructions; 3) The pLenti6.3/V5-DEST Gateway Vector Kit (Thermo #V53306) was used according to manufacturer’s instructions. Briefly, the hSIRT3 WT gBlock Gene Fragment was

(19) A CRISPR screen identifies MAPK7 as a target for combination with MEK inhibition in KRAS mutant NSCLCPLOS ONEJune 18, 2018Nicholas Dompe et al.10 μg/mL of Blasticidin. Viral constructs Cas9 was cloned into the pLENTI6.3 vector (ThermoFisher #V53306) which contains a Blasticidin selection marker. The gRNA vectors are based on Sigma’s pLKO1.5 lentiviral

(20) Exploiting AR-Regulated Drug Transport to Induce Sensitivity to the Survivin Inhibitor YM155Molecular Cancer ResearchMay 1, 2017Michael D. Nyquist, Alexandra Corella, John Burns, Ilsa Coleman, Shuai Gao, Robin Tharakan, Luke Riggan, Changmeng Cai, Eva Corey, Peter S. Nelson, et al.cat#K250020) then a gateway reaction was used to clone into the pL6.3/V5 lentivirus (ThermoFisher, V53306). The SLC35F2 shRNAs were the GIPZ Open Biosystems Human shRNAmirs V3LHS_377258 and V2LHS_154477. LuCaP

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pLenti6.3/V5-DEST Gateway Vector Kit from Thermo Fisher Scientific

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pLenti6.3/V5-DEST Gateway Vector Kit from Thermo Fisher Scientific

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