NEBNext Ultra II FS DNA PCR-Free Library Prep Kit with Sample Purification Beads E7435S from New England Biolabs

Product Specs
ItemNEBNext Ultra II FS DNA PCR-Free Library Prep Kit with Sample Purification Beads
CompanyNew England Biolabs
Price $667.00 Inquire
Catalog NumberE7435S
Quantity24 reactions
ApplicationsDNA Analysis, PCR, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Selenium-loaded sustained-release schizophyllan alleviates pancreatic and pulmonary inflammatory damage in type 1 diabetes mellitus by modulating gut microbiota and T cell balanceJournal of NanobiotechnologyMarch 14, 2026Junming Luo, Siying Pei, Zhiying Ai, Runqing Rao, Zhuoyuan Yang, Xin Guo, Zeyi Ai, Lilin Fan, Fang Zoupreparation. cDNA libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (E7435L, NEB, China), according to the manufacturer’s instructions, and their quality was evaluated on the Agilent

(2) Pharmacological activation of SIRT1–AMPK by ginsenoside Rb1: a novel therapeutic strategy for pressure injury via dual suppression of ferroptosis and inflammationFrontiers in PharmacologyFebruary 17, 2026Hongbo Zhu, Hang Li, Yinong Shi, Hua Zhi, Xuelu Zhao, Zhiwen Wang, Jinhui Liuvalues exceeded 7.0. RNA libraries were constructed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, E7435L, Beijing, China), compatible with the Illumina® sequencing platform, and then sequenced on the Illumina

(3) Mitochondria-targeted nanozyme system for psoriasis treatmentJournal of NanobiotechnologyFebruary 7, 2026Yan Mou, Yuan Ma, Xiaojun Yu, Yushu Wang, Shu Wang, Shuang Wangto construct cDNA libraries with the NEBNext Ultra II RNA Library Prep Kit for Illumina (Catalog No. E7435L, NEB, China), following the manufacturer’s protocol. Library quality was verified using the Bioanalyzer

(4) DNMT1 modulation of RASSF1A methylation enhances breast cancer brain metastasisCell Death & DiseaseDecember 11, 2025Qianqian Cao, Gongwen Xu, Guanghui Huang, Sheng Li, Hao Li, Xiaonan Zhang, Xiaomei LiDNA (cDNA) libraries were generated using the NEBNext® UltraTM RNA Library Prep Kit for Illumina® (E7435L, NEB, Beijing), and their quality was assessed with the Agilent Bioanalyzer 2100 system. Following the

(5) CircRNA circ_0004058 Modulates Early Brain Injury in Subarachnoid Hemorrhage Through miR-221-3p and VE1 Activation PathwayTranslational Stroke ResearchDecember 1, 2025Hua Gu, Yong Caiof total RNA per sample was processed using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (E7435L, New England Biolabs, Beijing, China). Indexed libraries were clustered on the cBot Cluster Generation

(6) Emerging role of HIC1 in prostate cancer progression and therapeutic response: A novel perspectiveJ Cell Commun SignalOctober 3, 2025Dun Xue, Long Tan, Fengshuai Yang, Xiaolan Tian, Qian Zuo, Xinghui WuRNA sample preparation. For cDNA library production, the NEBNext® UltraTM RNA Library Prep Kit (NEB, E7435L) designed for use with Illumina® was employed according to the manufacturer's instructions. The quality

(7) Modulation of ferroptosis via YY1-SLC7A11 axis in hepatic ischemia-reperfusion injury pathogenesisActa Biochimica et Biophysica SinicaSeptember 1, 2025Shaochuang Wang, Baofei Jiang, Jun Cao, Ting Xu, Chengming Zhou, Xiangyou Yu, Yi Wang, Yu Xie, Lindong Ji, Guangrong Zhou, et al.protocol, cDNA libraries were prepared via the NEBNext® UltraTM RNA library preparation kit for Illumina® (E7435L; NEB, Beijing, China) and evaluated for quality on a Bioanalyzer 2100 system (Agilent). The indexed

(8) WSGC@FA@PEG/PEI‐SPIONs Mitigate Chemoresistance in Gastric Adenocarcinoma by Modulating the Notch Signaling Pathway and MitophagyAdvanced ScienceSeptember 1, 2025Dongjian Song, Qiuliang Liu, Zechen Yan, Qi Wang, Meng Su, Hui Zhang, Longyan Shi, Yingzhong Fan, Qian Zhang, Heying Yang, et al.DNA (cDNA) libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, E7435L, CH), following the manufacturer's guidelines. Library quality was assessed using the Bioanalyzer 2100

(9) Timp2-modified gelatinhydroxyphenylpropionic acid hydrogels reverse enhanced scleral recovery and suppress myopia development in miceMaterials Today BioAugust 1, 2025Kaiqi Liu, Ting Li, Lingwen Gu, Zhenyu Jiang, Jing Jiang, Xi Yang, Yang Zhou, Xiao Feng, Qianqian Gong, Lihua Zhao, et al.RNA library preparation. Following the manufacturer's instructions, the NEBNext® UltraTM RNA Library Prep Kit (E7435L, NEB, USA) for Illumina® was used to generate cDNA libraries, which were evaluated for quality on an

(10) Targeting TNFAIP2 with NIR-II CRISPR-Cas9 nanosystem to overcome cisplatin resistance in laryngeal cancerNPJ Precis OncolJuly 29, 2025Xiaoli Li, Jiashuo Wang, Jiaxing Guo, Ming ZhangcDNA library construction, following the protocol of the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (E7435L, NEB, China). Library quality was confirmed using the Agilent Bioanalyzer 2100 system. Cluster generation

(11) Nanoparticle-mediated targeting of PGC-1α reveals critical metabolic pathways in bladder cancer metastasisCommun BiolMay 23, 2025Zhenghua Liu, Peng Xin, Weiwei Wu, Mingyue Jin, Yang Du, Yuanjun Jiang, Tao Liu, Hao Zhangsample preparation. Following the manufacturer’s instructions, the NEBNext® Ultra™ RNA Library Prep Kit (E7435L, NEB, Beijing, China) compatible with Illumina® (USA) was used to generate cDNA libraries, with library

(12) PTEN as a prognostic factor for radiotherapy plus immunotherapy response in nasopharyngeal carcinomaJ NanobiotechnologyApril 21, 2025Jiaxing Guo, Ming Zhang, Xiaoli Li, Jiashuo Wangfor RNA library preparation. Libraries were prepared using the NEBNext® UltraTM RNA Library Prep Kit (E7435L, NEB, Beijing) for Illumina® (The state of Nebraska, USA), recommended by the manufacturer, and their

(13) NS3 of hepatitis C virus drives hepatocellular carcinoma progression through a novel RNA-interference pathwayJ Cell Commun SignalApril 12, 2025Yajun Liang, Jian Luo, Liya Hu, Jun Zhangpreparation, 3 μg of total RNA per sample was processed using the NEBNext® Ultra™ RNA Library Prep Kit (NEB, E7435 L, China) for Illumina® sequencing. Library quality was evaluated with a Bioanalyzer 2100 system. Index‐coded

(14) Targeted nanoparticle delivery system for tumor-associated macrophage reprogramming to enhance TNBC therapyCell Biol ToxicolMarch 8, 2025Xiaoshen Dong, Xiaoou Wang, Xinyu Zheng, Haiyang Jiang, Lu Liu, Ningye Ma, Shuo Wangmanufacturer, the NEBNext® UltraTM RNA Library Preparation Kit designed for Illumina® sequencing (NEB, E7435L, Beijing, China) was applied to craft cDNA libraries. Subsequent quality assessments of these libraries

(15) LncRNA TCL6 regulates miR-876–5p/MYL2 axis to suppress breast cancer progressionTranslational OncologyMarch 1, 2025YaoBang Liu, Hong Li, DaHai Chai, Bin Lian, ZhengYang Bai, YaLi Gao, JinPing Lipreparation. Following the manufacturer's recommendations, the NEBNext®UltraTM RNA Library Prep Kit (NEB, E7435L, China) compatible with Illumina® (USA) was used to generate cDNA libraries, and the quality was evaluated

(16) Deciphering SPP1-related macrophage signaling in the pathogenesis of intervertebral disc degenerationCell Biol ToxicolJanuary 18, 2025Xiao-Jun Yu, Peng Zou, Tian-Qi Li, Xiao-Fan Bai, Shan-Xi Wang, Jian-Bin Guan, Yuan-Ting Zhao, Meng-Wei Li, Xiaodong Wang, Ying-Guang Wang, et al.producer, cDNA libraries were synthesized utilizing the NEBNext® UltraTM RNA Library Prep Kit (NEB, E7435L, Beijing, China) specifically designed for Illumina® sequencing, and the quality assessment was carried

(17) Targeting CHEK1: Ginsenosides-Rh2 and Cu2O@G-Rh2 nanoparticles in thyroid cancerCell Biol ToxicolJanuary 14, 2025Lidong Wang, Xin Wu, XinLu Wang, Meng Dong, Hao Zhang, Pengfei Zhaolibrary involved the utilization of the NEBNext® Ultra™ RNA Library Preparation Kit for Illumina® (NEB, E7435L, Beijing, China) and sequencing in accordance with established procedures (Arunachalam et al. 2022;

(18) HMGA1 influence on iron-induced cell death in Tfh cells of SLE patientsCell Biology and ToxicologyDecember 21, 2024Shan Zhao, Xiaotong Chen, Bohan Chang, Bailing TianRNA. The cDNA libraries were constructed employing the NEBNext®UltraTM RNA Library Preparation Kit (E7435L, NEB, Beijing, China) optimized for Illumina® sequencing as recommended by the manufacturer. Library

(19) CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo developmentMolecular BiomedicineDecember 13, 2024Qian Sun, Yanyu Li, Wen Yang, Wen Feng, Jiayun Zhou, Yijuan Cao, Bei Zhang, Zuobin Zhu, Conghui Han(Agilent, 5067–1511) [24, 25]. For mRNA sequencing, the NEBNext® UltraTM RNA Library Prep Kit (NEB, E7435L) was used, and libraries were generated on the Illumina-HiSeq 2000 platform. Paired-end reads were processed

(20) PU.1 regulation of type 1 dendritic cell function via NF-κB pathway in inhibition of non-small cell lung cancer progressionJournal of Pharmaceutical AnalysisNovember 22, 2024Tingting Wang, Yishuo Li, Qiongyu Duan, Chunlei Wang, Yixian Wang, Tianyu Hucomplementary DNA (cDNA) libraries were generated utilizing the NEBNext® UltraTM RNA Library Prep Kit (E7435L; New England BioLabs, Inc., Ipswich, MA, USA) suitable for Illumina® (Illumina, Inc., San Diego, CA,

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NEBNext Ultra II FS DNA PCR-Free Library Prep Kit with Sample Purification Beads

NEBNext Ultra II FS DNA PCR-Free Library Prep Kit with Sample Purification Beads from New England Biolabs

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NEBNext Ultra II FS DNA PCR-Free Library Prep Kit with Sample Purification Beads from New England Biolabs

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