Fig 1: Change in Protein Expression of Tissue Engineered Fibroblastic Reticular Cells (FRCs) Following Co‐culture with Immune Cells Isolated from Peripheral Blood. A) Flow cytometry derived UMAP plot of live cell populations in ADSCs, FRCP1, and FRCP2 cells with and without PBMC and mDCs after 15 days of co‐culture. Each plot represents at least 6 concatenated samples using two distinct ADSC donors and two distinct PBMC donors. Values below each UMAP represent the percentage of each subpopulation within the treatment condition. Colors correspond to the subpopulation in the heatmaps. B) Heatmap of the expression of FRC markers in each subpopulation. Values are normalized within each column relative to the specific marker C) concentration of CCL19, CXCL12, CXCL13, and IL‐7 secreted by samples after 15 days co‐culture. Experiments were performed with at least 6 replicates. Two distinct ADSC donors and two distinct PBMC donors were used. Data are shown as mean +/‐ SD.