Mitochondrial Membrane Potential Assay Kit (with JC-1) from Elabscience Bionovation Inc.

The decrease of mitochondrial membrane potential is a marker event in the early stage of apoptosis. It occurs before the appearance of nuclear apoptotic features (chromatin condensation and DNA fragmentation). Once the mitochondrial membrane potential collapses, apoptosis is irreversible. JC-1 is an ideal fluorescent probe widely used to detect mitochondrial membrane potential ∆Ψm. In normal cells, the mitochondrial membrane potential is high, JC-1 aggregates in the mitochondrial matrix to form a polymer, which can produce red fluorescence. In the early stage of apoptosis, the mitochondrial membrane potential decreases, JC-1 can’t accumulate in the mitochondrial matrix. When JC-1 is a monomer, it can produce green fluorescence. Elabscience® Mitochondrial Membrane Potential Assay Kit (with JC-1) is developed to identify early apoptotic by detecting changes in mitochondrial membrane potential using JC-1 as a fluorescent probe