KAPA RNA HyperPrep with RiboErase (HMR) 08098140702 from Roche

Product Specs
ItemKAPA RNA HyperPrep with RiboErase (HMR)
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number08098140702
Quantity96 libraries

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) CD146 + interstitial cells contribute to the dystrophic skeletal muscle phenotype in vitroScientific ReportsFebruary 24, 2026Bartosz Mierzejewski, Zuzanna Michalska, Dominika Kulma, Aleksandra Bos, Wladyslawa Streminska, Zbigniew Bartoszewicz, Edyta Brzoskaand library construction using the KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems, cat. no. 08098140702) according to the manufacturer’s protocol. The adapters used were KAPA_Univeral adapter and KAPA UDI

(2) NSD3 stabilizes nuclear compartmentalization and promotes megabase-scale chromatin interactionsbioRxivFebruary 14, 2026Yi-Hung Chen, John R. Collette, Krupa Sampat, Ivaylo D. Yonchev, Catherine E. Hawkins, Christopher Ponne, Celeste D. Rosencrance, Kyle P. Eagenribosomal RNA-depleted library preparation using KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche # 08098140702) conducted by Admera Health, LLC (South Plainfield, NJ). Mass spectrometry proteomics data processing Proteomics

(3) Restraint of cancer cell plasticity by spatial homotypic clusteringCancer CellDecember 8, 2025Simona Migliozzi, Bruno Adabbo, Luciano Garofano, Fan Wu, Pedro Davila, Ricardo J. Komotar, Michael E. Ivan, Ashish H. Shah, Benjamin B. Currall, Sion Williams, et al.Analyzer High Sensitivity NGS kits,Agilent,DNF-474-0500 KAPA RNA HyperPrep Kit with RiboErase,Roche,08098140702 10B Reagent Kit (300 Cycle),Illumina,20085594 CMx Hs 6K Discovery RNA Panel,Bruker,121500041 "CMx

(4) Phase I/II trial of encorafenib, cetuximab, and nivolumab in microsatellite stable, BRAFV600E metastatic colorectal cancerCancer CellNovember 10, 2025Van K. Morris, Christine M. Parseghian, Vahid Bahrambeigi, Nourhan Abdelfattah, Lianchun Xiao, Anjali Agrawal, Kangyu Lin, Kanwal P.S. Raghav, Robert A. Wolff, Arvind Dasari, et al.samples from twelve cases using the KAPA Stranded RNA-Seq kit with RiboErase (HMR) (KAPA, Roche, cat. # 08098140702) and libraries were sequenced on a NextSeq 500 sequencer (Illumina, San Diego, CA, USA). Raw reads quality

(5) Targeting histone H2B acetylated enhanceosomes via p300/CBP degradation in prostate cancerNat GenetOctober 3, 2025Jie Luo, Zhixiang Chen, Yuanyuan Qiao, Jean Ching-Yi Tien, Eleanor Young, Rahul Mannan, Somnath Mahapatra, Rupam Bhattacharyya, Lanbo Xiao, Tongchen He, et al.RNA was depleted using the RiboErase module of the KAPA RNA Hyper+RiboErase HMR Kit (Roche, cat. no. 08098140702), and the ribosomal RNA-depleted RNA was used for library preparation following the manufacturer’s protocol.

(6) CDK12/13 inactivation triggers STING-mediated antitumor immunity in preclinical modelsJournal of Clinical InvestigationSeptember 16, 2025Yi Bao, Yu Chang, Jean Ching-Yi Tien, Gabriel Cruz, Fan Yang, Rahul Mannan, Somnath Mahapatra, Radha Paturu, Xuhong Cao, Fengyun Su, et al.RNA sequencing, libraries were constructed with the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, 08098140702). Briefly, 800 ng of total RNA underwent ribosomal RNA depletion via enzymatic digestion, followed by

(7) Small round cell sarcoma tumoroid biobank reveals CIC::DUX4 sarcoma vulnerability to MCL-1 inhibitionNature communicationsAugust 21, 2025Ringnalda FCAS, van Son GJF, Verweij LHG, Kim SY, Amo-Addae V, Flucke UE, Hiemcke-Jiwa LS, Langenberg KPS, Bramer JAM, Heimans L, et al.described84. In short, cDNA libraries were generated using the KAPA RNA HyperPrep Kit with RiboErase (#08098140702, Roche) and sequenced on a NovaSeq 6000 (2 × 150 bp) (Illumina). Reads were mapped to the human26–31

(8) A new human in vitro model of cytotypic and testosterone-producing organoids derived from testicular tissue of transgender womenHum Reprod OpenAugust 20, 2025Samuel Madureira Silva, Frédéric Chalmel, Andrea Errico, Katerina Papageorgiou, Guillaume Richer, Edith Chan Sock Peng, Antoine D Rolland, Kelly Tilleman, Guy T'Sjoen, Ilaria Dando, et al.libraries were created from 150 ng of total RNA using the KAPA RNA HyperPrep kit with RiboErase HMR (08098140702, Roche Diagnostics), according to the manufacturer’s instructions. In summary, following ribodepletion

(9) Terminal nucleotidyltransferase Tent2 microRNA A-tailing enzyme regulates excitatory/inhibitory balance in the hippocampusRNAJune 1, 2025Patrycja Wardaszka-Pianka, Bozena Kuzniewska, Natalia GumiNska, Anna Hojka-Osinska, Monika Puchalska, Jacek Milek, Aleksandra Stawikowska, Pawel Krawczyk, Francois Philippe Pauzin, Tomasz Wojtowicz, et al.ribodepleted and the libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (KAPA Biosystems, 08098140702), according to the manufacturer's recommendations. For library preparation, 1 µg of RNA was used, fragmented

(10) Unraveling mutagenic processes influencing the tumor mutational patterns of individuals with constitutional mismatch repair deficiencyNat CommunMay 14, 2025Dilys D. Weijers, Snežana Hinić, Emma Kroeze, Mark Aj Gorris, Gerty Schreibelt, Sjors Middelkamp, Arjen R. Mensenkamp, Reno Bladergroen, Kiek Verrijp, Nicoline Hoogerbrugge, et al.sequencing51, for which libraries were prepared using KAPA RNA HyperPrep Kit with RiboErase (HMR) (Cat. No. 08098140702, Roche, Basel, Switzerland) according to manufacturer’s protocol. Fragmentation and priming were performed

(11) Isogenic comparison of Airn and Xist reveals core principles of Polycomb recruitment by lncRNAsMolecular CellMarch 20, 2025Jackson B. Trotman, Elizabeth W. Abrash, McKenzie M. Murvin, Aki K. Braceros, Shuang Li, Samuel P. Boyson, Ryan T. Salcido, Rachel E. Cherney, Steven R. Bischoff, Kyle Kaufmann, et al.replicates. RNA-Seq Libraries for RNA sequencing were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche 08098140702) and KAPA Unique Dual-Indexed Adapters (Roche 08861919702) or TruSeq adapters, with each library’s input

(12) Short-term fasting before living kidney donation has an immune-modulatory effectFrontiers in ImmunologyFebruary 20, 2025Christiaan A. J. Oudmaijer, Daphne S. J. Komninos, Rutger A. Ozinga, Kimberly Smit, Nina E. M. Rozendaal, Jan H. J. Hoeijmakers, Wilbert P. Vermeij, Joachim G. J. V. Aerts, Jan N. M. IJzermans, Marcella Willemsen(Thermo Fisher Scientific). RNAseq library was constructed using KAPA Robo erase Hyperprep (Roche; 08098140702), with subsequent quantification by the Qubit (Thermo Fisher Scientific) and TapeStation system (Agilent)

(13) The UBA1-STUB1 Axis Mediates Cancer Immune Escape and Resistance to Checkpoint BlockadeCancer discoveryFebruary 7, 2025Bao Y, Cruz G, Zhang Y, Qiao Y, Mannan R, Hu J, Yang F, Gondal M, Shahine M, Kang S, et al.(Agilent Technologies; #5067-1511), the KAPA RNA HyperPrep Kit with RiboErase (Roche Sequencing Solutions; catalog #08098140702) was used to build the libraries with a total of 800 ng RNA for each sample, following the user’s manual.

(14) Evolutionary innovations in germline biology of placental mammals identified by transcriptomics of first-wave spermatogenesis in opossumDevelopmental CellFebruary 1, 2025Kira L. Marshall, Daniel J. Stadtmauer, Jamie Maziarz, Günter P. Wagner, Bluma J. LeschKAPA mRNA HyperPrep Kit (Roche # 08098123702) or KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche # 08098140702) and sequenced on an Illumina NovaSeq to generate paired-end, 100 base pair read libraries at an average

(15) An interferon-stimulated long non-coding RNA USP30-AS1 as an immune modulator in influenza A virus infectionPLoS pathogensJanuary 1, 2025Cao Y, Chin AWH, Gu H, Li M, Gu Y, Lau SPN, Hui KPY, Chan MCW, Poon LLM.manufacturer’s instructions. cDNA library of purified RNA was prepared by using KAPA mRNA HyperPrep Kit (08098140702, Roche). RNA samples that had passed QC were collected using poly-T oligo-attached magnetic beads to

(16) Multi-omics analysis reveals distinct gene regulatory mechanisms between primary and organoid-derived human hepatocytesDisease Models & MechanismsJanuary 1, 2025Haoyu Wu, Annie S. P. Yang, Suzan Stelloo, Floris J. M. Roos, René H. M. te Morsche, Anne H. Verkerk, Maria V. Luna-Velez, Laura Wingens, Johannes H. W. de Wilt, Robert W. Sauerwein, et al.library construction, 100-250 ng total RNA was used with a KAPA RNA Hyper+RiboErase HMR Kit (Roche, 08098140702), following the manual. After library construction, libraries were quantified on a bio-analyzer using

(17) p300/CBP degradation is required to disable the active AR enhanceosome in prostate cancerbioRxivAugust 26, 2024Jie Luo, Zhixiang Chen, Yuanyuan Qiao, Jean Ching-Yi Ching-Yi Tien, Eleanor Young, Rahul Mannan, Somnath Mahapatra, Tongchen He, Sanjana Eyunni, Yuping Zhang, et al.total RNA samples using the RiboErase module of the KAPA RNA Hyper+RiboErase HMR Kit (Roche Cat. No. 08098140702). The rRNA-depleted RNA was then applied for library preparation. The procedure was conducted following

(18) Targeting the mSWI/SNF complex in POU2F-POU2AF transcription factor-driven malignanciesCancer CellAugust 12, 2024Tongchen He, Lanbo Xiao, Yuanyuan Qiao, Olaf Klingbeil, Eleanor Young, Xiaoli S. Wu, Rahul Mannan, Somnath Mahapatra, Esther Redin, Hanbyul Cho, et al.,Beckman Coulter,Cat#A63881 T4 DNA ligase,New England Biolabs,Cat#M0202L KAPA RNA Hyper+RiboErase HMR,Roche Diagnostics,Cat#08098140702 NEBNExt Multiplex Oligos for Illumina,New England Biolabs,Cat#E6440L KAPA Hyper Prep,Kapa Biosystems,Cat#KK8504 FastDigest

(19) Transcriptomic Profiling of Plasma Extracellular Vesicles Enables Reliable Annotation of the Cancer-Specific Transcriptome and Molecular SubtypeCancer ResearchMay 15, 2024Vahid Bahrambeigi, Jaewon J. Lee, Vittorio Branchi, Kimal I. Rajapakshe, Zhichao Xu, Naishu Kui, Jason T. Henry, Wang Kun, Bret M. Stephens, Sarah Dhebat, et al.samples were processed using the KAPA Stranded RNA-Seq kit with RiboErase (HMR) (KAPA, Roche, cat. # 08098140702). Libraries were constructed according to the manufacturer protocol and were then quantified using an

(20) Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to viral infectionRNA BiologyDecember 31, 2023Zachary B Madaj, Michael S. Dahabieh, Vijayvardhan Kamalumpundi, Brejnev Muhire, J. Pettinga, Rebecca A. Siwicki, Abigail E. Ellis, Christine Isaguirre, Martha L. Escobar Galvis, Lisa DeCamp, et al.quality control, stranded RNA libraries were prepared from 500 ng RNA using the KAPA – mRNA Hyper Prep (08098140702, Roche). RNA libraries were then normalized, pooled and sequenced on Illumina NovaSeq S1 flow cell using

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KAPA RNA HyperPrep with RiboErase (HMR) from Roche

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KAPA RNA HyperPrep with RiboErase (HMR) from Roche

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