Alt-R™ Genome Editing Detection Kit 1075931 from Integrated DNA Technologies

Product Specs
ItemAlt-R™ Genome Editing Detection Kit
CompanyIntegrated DNA Technologies
Price Supplier Page View Company Product Page
Catalog Number1075931
ApplicationsGene Expression and Molecular Cloning, Genome editing, CRISPR
TypeCRISPR/Cas9 Vectors

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Integrated DNA Technologies

1710 Commercial Park

Coralville, Iowa 52241

United States

Phone: 800-328-2661

Company Profile

Website: http://www.idtdna.com

(1) Accessibility at a primed distal Fshb-Kcna4 super-enhancer is facilitated by Foxl2 during gonadotrope differentiationEndocrinologyApril 7, 2026Tal Refael, Gil Golan, Daniella Darsa, Lilach Pnueli, Probir Chakravarty, Karine Rizzoti, Philippa Melamedformation. This mixture was then analyzed using the Alt-R Genome Editing Detection Kit (T7E1 assay # 1075931, IDT) according to the manufacturer's instructions for T7 Endonuclease I digestion. Clones exhibiting

(2) Mitotic chromatin compaction tethers extrachromosomal DNA to chromosomes and prevents their mis-segregation into micronucleiJournal of Biological ChemistryFebruary 1, 2026Lu M. Yangheteroduplex formation and T7 endonuclease I digestion using the Alt-R Genome Editing Detection Kit (IDT 1075931) following manufacturer’s instructions. PCR primers: MKI67 exon 2 F 5′-GACTTGACGAGCGGTGGTTC-3′, R 5′-GCACCAAGGAAAAGTGACGG-3′;

(3) High CDC20 levels increase sensitivity of cancer cells to MPS1 inhibitorsEMBO ReportsJanuary 21, 2025Siqi Zheng, Linoy Raz, Lin Zhou, Yael Cohen-Sharir, Ruifang Tian, Marica Rosaria Ippolito, Sara Gianotti, Ron Saad, Rene Wardenaar, Mathilde Broekhuis, et al.activity was confirmed using the Alt-R™ Genome Editing Detection Kit (Integrated DNA Technologies, 1075931). To introduce the sgRNA library, 377 million iCas9-expressing 3T3 cells were transduced with the Mouse

(4) Iron Chelation Therapy Elicits Innate Immune Control of Metastatic Ovarian CancerCancer discoveryOctober 4, 2024Sandoval TA, Salvagno C, Chae CS, Awasthi D, Giovanelli P, Marin Falco M, Hwang SM, Teran-Cabanillas E, Suominen L, Yamazaki T, et al.sgRNA-Cas9 complexes using the Neon transfection system, according to the manufacturer’s protocol (IDT, Cat# 1075931). All materials for sgRNA-Cas9 complex generation were purchased from Integrated DNA Technologies (IDT)

(5) Validation of human telomere length multi-ancestry meta-analysis association signals identifies POP5 and KBTBD6 as human telomere length regulation genesNature CommunicationsMay 24, 2024Rebecca Keener, Surya B. Chhetri, Carla J. Connelly, Margaret A. Taub, Matthew P. Conomos, Joshua Weinstock, Bohan Ni, Benjamin Strober, Stella Aslibekyan, Paul L. Auer, et al.(QE09050; Epicentre) following the protocol recommended in the Alt-R genomic editing detection kit (1075931; IDT). Target editing regions were amplified (primers described in Supplementary Data 15, diagrams in

(6) PI3Kδ Inhibition Potentiates Glucocorticoids in B-lymphoblastic Leukemia by Decreasing Receptor Phosphorylation and Enhancing Gene RegulationCancers (Basel)December 27, 2023Jessica A. O. Zimmerman, Mimi Fang, Miles A. Pufall#V4XC-2032)). After 48–72 h, editing efficiency was checked by T7EI digest (IDT, Coralville, IA, USA, #1075931). Cells were single-cell sorted (Becton Dickinson Aria II) into 96 well plates. Positive clones were

(7) Integration of xeno-free single-cell cloning in CRISPR-mediated DNA editing of human iPSCs improves homogeneity and methodological efficiency of cellular disease modelingStem Cell ReportsDecember 12, 2023Atefeh Namipashaki, Kealan Pugsley, Xiaodong Liu, Kirra Abrehart, Sue Mei Lim, Guizhi Sun, Marco J. Herold, Jose M. Polo, Mark A. Bellgrove, Ziarih Hawiendonuclease 1 enzyme using the Alt-R Genome Editing Detection Kit (Integrated DNA Technologies, cat. no. 1075931). The cleaved DNA fragments were run on an Agilent Fragment Analyzer system and analyzed with the ProSize

(8) PI3Kδ inhibition potentiates glucocorticoids in B-lymphoblastic leukemia by decreasing receptor phosphorylation and enhancing gene regulationbioRxivAugust 11, 2023Jessica A.O. Zimmerman, Mimi Fang, Miles A. Pufall(SF Cell Line 4D-Nucleofector™ X Kit S (Lonza, #V4XC-2032)). After 48–72 hours, editing efficiency was checked by T7EI digest (IDT, #1075931). Cells were single-cell sorted (Becton Dickinson Aria II) into 96 well plates. Positive clones were

(9) Upregulation of SYNGAP1 expression in mice and human neurons by redirecting alternative splicingNeuronMay 17, 2023Yang R, Feng X, Arias-Cavieres A, Mitchell RM, Polo A, Hu K, Zhong R, Qi C, Zhang RS, Westneat N, et al.K0721 TruSeq RNA Library Prep Kit,Illumina,Cat# RS-122-2001 Alt-R Genome Editing Detection Kit,IDT,Cat# 1075931 SuperScript IV,Thermo-Fisher,Cat# 18091050 Gibson Assembly Master Mix,NEB,Cat# E2611S Quick Ligation

(10) Utilizing an Endogenous Progesterone Receptor Reporter Gene for Drug Screening and Mechanistic Study in Endometrial CancerCancers (Basel)October 6, 2022Yiyang Li, Wei Zhou, Xiangbing Meng, Sarina D. Murray, Long Li, Abby Fronk, Vanessa J. Lazaro-Camp, Kuo-kuang Wen, Meng Wu, Adam Dupuy, et al.target sgRNA sequence and the adjacent sequence, followed by Alt-R genome editing detection assay (cat# 1075931, IDT), per the manufacturer’s instructions. Briefly, the PCR products from the wildtype PGR were mixed

(11) Mitochondrial dynamics regulate genome stability via control of caspase-dependent DNA damageDevelopmental cellMay 23, 2022Cao K, Riley JS, Heilig R, Montes-Gómez AE, Vringer E, Berthenet K, Cloix C, Elmasry Y, Spiller DG, Ichim G, et al.assays,Critical commercial assays,Critical commercial assays ,, Alt-R Genome Editing Detection Kit,IDT,Cat# 1075931 ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, "Mouse

(12) Investigating CRISPR/Cas9 gene drive for production of disease-preventing prion gene allelesPloS oneJanuary 1, 2022Castle AR, Wohlgemuth S, Arce L, Westaway D.performed according to the Alt-R Genome Editing Detection Kit instructions (Integrated DNA Technologies, 1075931), except that incubation with T7E1 was shortened to 30 min. Reactions were stopped by addition of EDTA

(13) CSNK1A1, KDM2A, and LTB4R2 Are New Druggable Vulnerabilities in Lung CancerCancers (Basel)July 12, 2021Elisabetta Sauta et al.described to obtain the KO cell lines of target genes [11]. An Alt-R® genome editing detection kit (#1075931, IDT, Skokie, IL, USA) was used to confirm on-target CRISPR event efficiency. Sequences of sgRNAs target

(14) Highly Efficient CRISPR-Associated Protein 9 Ribonucleoprotein-Based Genome Editing in Euglena gracilisSTAR ProtocolsOctober 28, 2020Toshihisa Nomura, Mizuki Yoshikawa, Kengo Suzuki, Keiichi MochidaCloneJET PCR Cloning Kit,Thermo Scientific,K1231 Alt-R Genome Editing Detection Kit,Integrated DNA Technologies,1075931 (NH4)2HPO4,FUJIFILM Wako Pure Chemical Corporation,016-03325 KH2PO4,FUJIFILM Wako Pure Chemical Corporation,169-04245 MgSO4・7H2O,FUJIFILM

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7/25/2023Genome Editing Efficiency Determination by T7 Endonuclease I (T7EI) Mismatch Cleavage Assay Ganesh PusapatiI routinely use CRISPR/Cas9-based methodology to generate gene knockouts in cell lines. As a first step, I do pooled gene knockout using the lentiCRISPR v2 plasmid and test the editing efficiency ... Read Review

Alt-R™ Genome Editing Detection Kit from Integrated DNA Technologies

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Alt-R™ Genome Editing Detection Kit from Integrated DNA Technologies

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