Fig 1: Albumin sequence obtained by LC-MS/MS.A). Unique peptide “LSQTFPNADFAEITK” levels in Alb-/- and WT mice. Analysis by student t-test. n = 6 per group. Data are presented as the mean ± standard error. B). Amino acid sequence coverage for albumin in proteomics analysis. Blue highlights: all peptides identified in WT mice, 482 amino acids, 79% coverage. Bold letters: unique peptides identified in all WT replicates, 342 amino acids; 56% coverage. Underlined letters: unique peptides identified in all albumin Alb-/- replicates, 15 amino acids; 2.5% coverage. Unique peptides: peptides that are unique to murine albumin, with no overlap with other protein hits (such as human albumin, bovine albumin, and murine alpha-fetoprotein).
Fig 2: Coomassie staining of sodium dodecyl sulfate-polyacrylamide electrophoresis gel for plasma proteins in mice.Human serum albumin (HSA) and molecular weight markers were used as a reference. Alb-/- mice showed a drastic reduction in protein abundance at the molecular weight of albumin (66.5 kDa), while WT mice presented a dark band at the same position.
Supplier Page from Abcam for BCP Albumin Assay Kit