Nitric Oxide Assay Kit ab272517 from Abcam

Product Specs
ItemNitric Oxide Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab272517
Quantity100 Test
Detection Range0.6 - 100 µM

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Cambridge Biomedical Campus

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+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Increasing standardized ileal digestible arginine-to-lysine ratios improves growth performance and carcass characteristics in 10- to 31-d-old broilersPoultry ScienceApril 28, 2026Inho Cho, Jisoo Tak, Changsu Kongfor determination of nitric oxide (NO) concentration using commercial nitric oxide assay kits (Abcam, ab272517, Cambridge, Cambridgeshire, United Kingdom). In this method, total nitrate and nitrite (NO₃⁻/NO₂⁻) derived

(2) High-intensity irreversible electroporation targeting intracellular structures enhance tumor ablation in lung cancer modelsPLoS OneApril 22, 2026Hong Bae Kim, Jin Young Youm, Joon-Mo Yang, Sung Bo Simhydrogen peroxide levels Intracellular nitrogen oxide (NO) levels were measured using the NO Assay Kit (ab272517, Abcam, Cambridge, UK). Cells in 500 µL of the culture media were exposed to experimental conditions

(3) Ganoderma lucidum mediates microglial polarization and ameliorates experimental autoimmune encephalomyelitis by reducing oxidative stress and inhibiting NF-κB/STAT3 pathwayChin MedApril 10, 2026Lu Zhang, Jie Chen, Sauchu Yuen, Meiling Wu, Wenting Li, Shenyu Yan, Jiangang ShenFor RNS-released level determination, the Nitric Oxide Assay Kit reagent was purchased from Abcam (ab272517, Abcam). 4,5-diaminofluorescein diacetate (DAF-2 DA; Sigma, USA), and hydroethidine (HEt, Sigma, USA)

(4) Comparative Effects of Dexamethasone and ASC Secretome in an Ex Vivo Osteoarthritis Co-Culture ModelBiology (Basel)March 20, 2026Elena Della Morte, Francesca Cadelano, Andrea Pasquini, Luigi Zagra, Alessandro Baj, Chiara Giannasi, Stefania Niadaquantification of nitric oxide (NO) levels was performed utilizing the Nitric Oxide Assay kit from Abcam (ab272517, Abcam, Cambridge, UK). Undiluted samples underwent processing in accordance with the provided protocol.

(5) Fenofibrate attenuates the adverse effects of radiation on endothelial cells through modulation of ROS-NO signalling and inflammationRedox BiologyFebruary 1, 2026Omar Elsaeed, Christine von Toerne, Prabal Subedi, Louis Wilhelm, Lukas Duchrow, Ali Bashiri Dezfouli, Gabriele Multhoff, Simone Moertl, Omid AzimzadehNitric oxide measurement The intracellular NO was measured by the Nitric Oxide Assay Kit (Abcam, UK, ab272517) as recommended by the manufacturer. The results were normalized to 100 μg protein input per sample. 5.14

(6) Photobiomodulation stimulates mitochondrial function and cell proliferation in meniscus-derived stem cells (MeSCs) via activation of TRPV1 channelSci RepDecember 4, 2025Jiabei Tong, Xiaoyun Wu, Zifan Wang, Xiujuan Li, Yang Yu, Zhong Zhang, Zhaohui Zheng, Tan Huang, Zhijie Maconcentration measurements The NO concentrations were measured at 12 h after irradiation using a kit (ab272517, Abcam) as directed. Cells were harvested and centrifuged as described above, with the resulting supernatant

(7) Carboxypeptidase D deficiency causes hearing loss amenable to treatmentJ Clin InvestSeptember 30, 2025Memoona Ramzan, Natalie Ortiz-Vega, Mohammad Faraz Zafeer, Amanda G. Lobato, Tahir Atik, Clemer Abad, Nirmal Vadgama, Duygu Duman, Nazım Bozan, Enise Avcı Durmuşalioǧlu, et al.minutes at 60°C, and absorbance was measured at 540 nm according to an NO assay kit (Abcam, catalog ab272517). For cGMP levels, cells were harvested after 48 hours in 0.1 M HCl and hydrolyzed to obtain total cell

(8) Metformin promotes PEN2 expression to attenuate microglia-mediated neurotoxicity induced by HIV-1 TatJournal of neurovirologyAugust 1, 2025Shen Y, Xu T, Sun Y, Zhang K, Cao X, Shen L, Tang M.565 nm. The nitric oxide (NO) concentration in microglia was measured with an NO assay kit (Abcam, Ab272517) according to the manufacturer’s instructions. Statistical analyses All quantifications were conducted

(9) Nitroglycerin-responsive gene switch for the on-demand production of therapeutic proteinsNature Biomedical EngineeringFebruary 14, 2025Mohamed Mahameed, Shuai Xue, Benjamin Danuser, Ghislaine Charpin-El Hamri, Mingqi Xie, Martin Fusseneggertissue NO in tissue was quantified using a Nitric Oxide Assay Kit (Abcam, catalogue number 521-9100 ab272517) according to the manufacturer’s instructions. Statistical analysis The statistical significance of

(10) Aging‐Associated Liver Sinusoidal Endothelial Cells Dysfunction Aggravates the Progression of Metabolic Dysfunction‐Associated Steatotic Liver DiseaseAging CellFebruary 6, 2025Qingqing Dai, Quratul Ain, Navodita Seth, Hongchuan Zhao, Michael Rooney, Alexander ZipprichOxide Measurement The intracellular nitrite concentrations were quantified by NO Assay Kit (Abcam, ab272517) to access intracellular NO levels according to the kit's manual. Subsequently, the measured nitrite

(11) The role of low-carbohydrate, high-fat diet in modulating autophagy and endoplasmic reticulum stress in aortic endothelial dysfunction of metabolic syndrome animal modelFrontiers in NutritionNovember 13, 2024Basmah Eldakhakhny, Abdulhadi Bima, Aliaa A. Alamoudi, Abrar Alnami, Salwa Mohamed Abo-Elkhair, Hussein Sakr, Yousef Almoghrabi, Fatma Mohamed Ghoneim, Reham Mohamed Nagib, Ayman Elsamanoudywas also estimated by the colorimetric method (Nitric Oxide Assay Kit, Abcam Co., Boston, MA, USA, ab272517), and the results were expressed as μmol/L Ghoneim et al. (94). According to the manufacturer’s instructions,

(12) Cartilage responses to inflammatory stimuli and adipose stem/stromal cell-derived conditioned medium: Results from an ex vivo modelRegenerative TherapyJune 28, 2024Francesca Cadelano, Elena Della Morte, Stefania Niada, Francesco Anzano, Luigi Zagra, Chiara Giannasi, Anna Teresa Maria Briniquantification of nitric oxide (NO) levels was performed utilizing the Nitric Oxide Assay kit from Abcam (ab272517, Abcam, Cambridge, UK). Undiluted samples underwent processing in accordance with the provided protocol.

(13) Role of Tyrosine Nitrosylation in Stress-Induced Major Depressive Disorder: Mechanisms and ImplicationsInternational Journal of Molecular SciencesSeptember 27, 2023Gregory C. Wilson, Simone Keitsch, Matthias Soddemann, Barbara Wilker, Michael J. Edwards, Erich Gulbinsin stressed mice is caused by increased NO. Nitric oxide assay kit was from Abcam, Cambridge, UK (#ab272517) Enzyme assays in the hippocampus were carried out to determine mechanisms of tyrosine nitrosylation: 1.

(14) Autophagy protein 5 controls flow-dependent endothelial functionsCellular and Molecular Life SciencesAugust 1, 2023Pierre Nivoit, Thomas Mathivet, Junxi Wu, Yann Salemkour, Devanarayanan Siva Sankar, Véronique Baudrie, Jennifer Bourreau, Anne-Laure Guihot, Emilie Vessieres, Mathilde Lemitre, et al.was measured on fresh plasma using an improved Griess method according to manufacturer’s protocol (ab272517, Abcam, The Netherlands). Deproteination was performed prior to assay according to manufacturer’s protocol. Proteomic

(15) Riluzole, a Derivative of Benzothiazole as a Potential Anti-Amoebic Agent against Entamoeba histolyticaPharmaceuticals (Basel)June 19, 2023Maritza Velásquez-Torres, José Guadalupe Trujillo-Ferrara, Marycarmen Godínez-Victoria, Rosa Adriana Jarillo-Luna, Víctor Tsutsumi, Virginia Sánchez-Monroy, Araceli Posadas-Mondragón, Roberto Issac Cuevas-Hernández, José Angel Santiago-Cruz, Judith Pacheco-Yépezfilters. 4.7. Determination of Nitric Oxide (NO) The NO production was determined using a NO assay kit (Ab272517, Abcam, Cambridge, UK) according to the manufacturer’s instructions. In brief, trophozoites were cultured

(16) Milk-derived extracellular vesicles protect intestinal barrier integrity in the gut-liver axisScience advancesApril 14, 2023Tong L, Zhang S, Liu Q, Huang C, Hao H, Tan MS, Yu X, Lou CKL, Huang R, Zhang Z, et al.NO were determined using a Nitric Oxide assay kit according to the manufacturer’s protocol (Abcam, ab272517). Colon and liver histology Colon and liver tissues were fixed in 10% formalin solution (Sigma-Aldrich,

(17) Lipidomics of Cell Secretome Combined with the Study of Selected Bioactive Lipids in an In Vitro Model of OsteoarthritisStem Cells Translational MedicineSeptember 21, 2022Sara Casati, Chiara Giannasi, Stefania Niada, Elena Della Morte, Marica Orioli, Anna T Briniin CH culture media following the reduction of nitrates to nitrites using an improved Griess method (ab272517 Nitric Oxide Assay Kit, Abcam, Cambridge, Regno Unito). Absorbance was measured at 540 nm. Nitrites

(18) α-Lipoic Acid Protects against Cyclosporine A-Induced Hepatic Toxicity in Rats: Effect on Oxidative Stress, Inflammation, and ApoptosisToxicsAugust 2, 2022Eman M. El-Mancy, Dalia Mahmoud Abdelmonem Elsherbini, Rasha Hamed Al-Serwi, Mohamed El-Sherbiny, Gehan Ahmed Shaker, Abdel-Moneim Hafez Abdel-Moneim, Eman T. Enan, Nehal M. Elsherbinyserum nitric oxide (NO) was measured by the colorimetric method (Nitric Oxide Assay Kit, Abcam co. ab272517), and the results were expressed as μmol/L. Additionally, activity of superoxide dismutase (SOD) and

(19) Human Osteochondral Explants as an Ex Vivo Model of Osteoarthritis for the Assessment of a Novel Class of OrthobiologicsPharmaceuticsJune 10, 2022Giannasi C, Mangiavini L, Niada S, Colombo A, Della Morte E, Vismara V, Ambrosanio A, Savadori P, Casati S, Peretti GM, et al.N-acylethanolamines. NO was measured using an improved Griess method based on the reduction of nitrates to nitrites (ab272517, Abcam, Cambridge, UK). Absorbance at 540 nm was measured with a Wallac Victor II microplate reader

(20) Evaluation of Endothelial Dysfunction and Autophagy in Fibromyalgia-Related Vascular and Cerebral Cortical Changes and the Ameliorative Effect of FisetinCellsDecember 24, 2021Fatma Mohamed Ghoneim, Salwa Mohamed Abo-Elkhair, Ayman Zaky Elsamanoudy, Dalia A. Shabaanoxide (NO) was measured by the colorimetric method (Nitric Oxide Assay Kit, Abcam Co., Boston, MA, USA, ab272517), and the results were expressed as μmol/L. 2.8. Statistical Analysis Comparisons were performed by

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Nitric Oxide Assay Kit from Abcam

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