Fig 1: Characterization and concentration of EVs isolated from second trimester maternal plasma and childhood scores on the SCQ.(A) Transmission electron microscopy image of a small extracellular vesicle (exosome) isolated from maternal plasma. (B) CD63 concentration in EVs from maternal plasma from mothers of children at low risk versus high risk based on total SCQ scores at age 4.5–6 years. Error bars represent SEM. (C) Mean EV concentration as determined by nanoparticle tracking analysis in maternal plasma from mothers of children at low risk versus high risk based on total SCQ scores. (D) Mean particle size in maternal plasma from mothers of children at low risk versus high risk based on total SCQ scores. (E) A comparison of the distribution of EVs across 10 nm bins between children at low risk versus high risk in the full sample, among male children, and among female children at age 4.5–6 years. Mean ± SEM; An asterisk denotes a significant group difference of p<0.05.