Opal 6-Plex Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Automated Detection Kit) NEL871001KT from Akoya Biosciences, a Quanterix company

Product Specs
ItemOpal 6-Plex Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Automated Detection Kit)
CompanyAkoya Biosciences, a Quanterix company
Price $2,278.10Supplier Page View Company Product Page
Catalog NumberNEL871001KT
Quantity1
ApplicationsMultiplex IHC
ReactivityHuman

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

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Website: quanterix.com

(1) IL-15 signaling via cis- and trans-presentation to progenitor-exhausted CD8+ T cells enhances radio-immunotherapy efficacy in ESCCJ NanobiotechnologyDecember 1, 2025Yi You, Linrui Gao, Shijia Li, Hui Huang, Tierun Wang, Zongchang Nie, Jian Zhou, Xiaoxue Ma, Jiarui Li, Hongyu Bie, et al.CTLA4, HAVCR2), Tem (IL7R, GPR183). Multiplex immunohistochemistry (mIF) The Opal Detection Kits (NEL871001KT) were purchased from Akoya Biosciences, USA. We performed a 4 or 7-cycle staining procedure, as recommended

(2) Dual blockades of TIM-3 and PD-1 effectively prevent hyper-progression and enhance the efficacy of anti-PD-1 therapy in high-grade serous ovarian cancerCell Death & DiseaseNovember 28, 2025Jie Li, Ying Zhou, Yahan Song, Geyang Dai, Xi Li, Yue Sun, Jing Wang, Rui Wei, Fei Li, Ling Xiconcentration of 1:400 for 1 h at room temperature. For IF, OPAL Multiplex 7-color IHC kit (Akoya Biosciences, NEL871001KT) was used according to the manufacturer’s instruction, followed by incubation with DAPI (Servicebio,

(3) NSD2 targeting reverses plasticity and drug resistance in prostate cancerNatureNovember 26, 2025Jia J. Li, Alessandro Vasciaveo, Dimitris Karagiannis, Zhen Sun, Kristjan H. Gretarsson, Xiao Chen, Ouathek Ouerfelli, Fabio Socciarelli, Ziv Frankenstein, Hanyang Dong, et al.signal-to-noise ratio. Multiplexed staining was performed using an Opal 6-plex detection kit (Akoya Biosciences, NEL871001KT) on a Bond Rx Research Stainer (Leica Biosystems), adding DAPI as a nuclear marker. Slides were imaged

(4) Microbial signals in primary and metastatic brain tumorsNature MedicineNovember 14, 2025Golnaz Morad, Ashish V. Damania, Brenda Melendez, Bharat B. Singh, Fabiana J. Veguilla, Rebecca A. Soto, Yasmine M. Hoballah, Pranoti V. Sahasrabhojane, Matthew C. Wong, Mona M. Ahmed, et al.staining was conducted using the Leica BOND RX autostainer and the Opal 7-color kit (Akoya Biosciences, NEL871001KT). Two separate lymphoid and myeloid panels were used for staining both glioma and BrM TMAs. The following

(5) Lead-in therapy targeting PD1 and/or LAG3 imposes distinct immune phenotypes in first-line treatment of metastatic melanomamedRxivNovember 8, 2025Lilit Karapetyan, Anthony R. Cillo, Shuaichao Wang, Arivarasan Karunamurthy, Ryan C. Massa, Anjali Rohatgi, Christopher Deitrick, Yana G. Najjar, Diwakar Davar, Jason J. Luke, et al.Bond RX. All staining reagents were provided in the Opal 6-Plex Detection Kit (Akoya Bioscience, cat# NEL871001KT), which was used according to the manufacturer’s instructions for sequential staining of each antibody

(6) Effect of tumor draining lymph nodes in the formation and maturation of tertiary lymphoid structure in patients with lung adenocarcinomaBMC CancerOctober 3, 2025Junxu Wen, Wenhua Yun, Xiaoyan Yin, Xiangjiao MengMultiplex immunofluorescence staining was performed using the Akoya Opal Kit (Akoya Biosciences, USA; NEL871001KT). Each slide was incubated overnight at 55 °C, followed by dewaxing with xylene and rehydration with

(7) Spatial and maturity heterogeneity of tertiary lymphoid structures shapes immune microenvironment and progression in prostate cancerJournal of the National Cancer CenterOctober 1, 2025Zhongyuan Wang, Qintao Ge, Ren Mo, Jiahe Lu, Xi Tian, Aihetaimujiang Anwaier, Shiqi Ye, Siqi Zhou, Weihang Guo, Chuanhai Cai, et al.established protocols for mIHC. The mIHC procedure utilized the Akoya OPAL Polaris 7-Color Automated IHC Kit (NEL871001KT) with the following panels: Panel 1: CD8, Cytokeratin (CK), PD-L1, DAPI; Panel 2: CD45, CD23, CD20,

(8) Tumor immune microenvironment analysis in different pathologic responses to neoadjuvant immunotherapy combined with chemotherapy in non-small cell lung cancerTranslational Lung Cancer ResearchSeptember 30, 2025Zhaofeng Wang, Yunchang Meng, Fang Zhang, Ping Zhan, Tangfeng Lv, Yong Song, Hongbing Liuon formalin-fixed paraffin-embedded (FFPE) tissue sections using the OPAL Polaris 7-color IHC kit (NEL871001KT, Akoya Biosciences, Marlborough, USA). Following deparaffinization and rehydration with the automated

(9) Leveraging the Immune Response from LIFE Biomaterial and Photon-Flash in Pre-Clinical Pancreatic Cancer TreatmentPharmaceuticsSeptember 29, 2025Michele Moreau, Katelyn Kelly, Serena Mao, Debarghya China, Girmachew Wasihun, Aditya Pandya, MohammadAli Tajik-Mansoury, Daniel Sforza, Devin Miles, Amol K. Narang, et al.(catalog# 7017936001 and 7017812001, Roche Diagnostics, Indianapolis, IN, USA), followed by OPAL 520 (NEL871001KT, Akoya Biosciences, Marlborough, MA, USA) diluted 1:150 in 1X Plus Amplification Diluent (catalog #

(10) Anlotinib plus sintilimab as first-line treatment for patients with advanced colorectal cancer (APICAL-CRC): an open-label, single-arm, phase II trialSignal Transduction and Targeted TherapySeptember 16, 2025Zhan Wang, Bao-Dong Qin, Chen-Yang Ye, Miao-Miao Wang, Ling-Yan Yuan, Hou-Shan Yao, Xiao-Dong Jiao, Ke Liu, Wen-Li Zhou, Wen-Xing Qin, et al.evaluated by multiplex immunofluorescence (mIF) using the Akoya OPAL Polaris 7-Color Automation IHC Kit (NEL871001KT). For further details regarding these analytical approaches, see the Supplementary Appendix. Outcomes The

(11) Cytotoxic NK Cells Impede Response to Checkpoint Immunotherapy in Melanoma with an Immune-Excluded PhenotypeCancer discoverySeptember 4, 2025Pozniak J, Roda N, Landeloos E, Antoranz A, Van Herck Y, De Visscher A, Demaerel PG, Vanwynsberghe L, Declercq J, Gkemisis C, et al.(Opal 6-Plex Detection Kit for Whole Slide Imaging – formerly Opal Polaris 7 Color IHC Automated Detection Kit – Akoya Biosciences, cat. #NEL871001KT). Two different panels were used: (i) NKp46 Opal 520, RRID: AB_355192; CD3ε Opal 570, RRID: AB_443425;

(12) BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell statesNature metabolismSeptember 1, 2025Rizzollo F, Escamilla-Ayala A, Fattorelli N, Lysiak NB, More S, Hernández Varas P, Barazzuol L, Van den Haute C, Van Asselberghs J, Nittner D, et al.Cell Signalling, 9367). Furthermore, the Opal 6-Plex Detection Kit - for Whole Slide Imaging (Akoya, NEL871001KT) was used for tyramide signal amplification, following the manufacturer’s protocol. For introduction

(13) Senescent fibroblasts secrete CTHRC1 to promote cancer stemness in hepatocellular carcinomaCell communication and signaling : CCSAugust 25, 2025Huang H, Peng W, Zhou Q, Zhao Y, Liu L, Cui H, Liang J, Cao M, Chen W, Wang R, et al.sc-1661), and CTHRC1 (1:1000, Proteintech, 16534-1-AP) with the Opal Polaris 3-Color IHC Detection Kit (NEL871001KT, AKOYA) following iterative cycles of staining, imaging, and antibody stripping. Fluorescence imaging

(14) Collagen gene signature in the tumor microenvironment predicts survival and guides prognosis in bladder cancerDiscov OncolAugust 6, 2025Yi Huang, Shuogui Fang, Weibin Xie, Yitong Zou, Hui Zhuo, Gang Shen, Hua Zhou, ChunPing Mao, Cong Lai, Jianqiu Kong, et al.accordance with the manufacturer's protocol using the Akoya OPAL Polaris 7-Color Automation IHC reagent (NEL871001KT). Then, FFPE samples were treated with primary antibodies P4H4, C1QTNF6, COLGALT1, COL4A1, COL14A1,

(15) Immunogenicity of autologous and allogeneic human primary cholangiocyte organoid cellular therapiesCell Rep MedJuly 15, 2025Sandra Petrus-Reurer, Winnie Lei, Olivia Tysoe, Maelle Mairesse, Adrian Baez-Ortega, Julia Jones, Thomas Tan, Sylvia Rehakova, Krishnaa T. Mahbubani, Cara Brodie, et al.conjunction with Akoya Biosciences Opal 6-Plex Detection Kit – for Whole Slide Imaging (reference: NEL871001KT). The sequence of staining and specific details can be found in Table S7. Finally, sections were counterstained

(16) Radiation therapy results in preferential tumor antigen-specific lymphodepletion in head and neck cancerNat CommunJuly 1, 2025Joseph Zenga, Musaddiq J. Awan, Anne Frei, Jamie Foeckler, Rachel Kuehn, Julia Kasprzak, Becky Massey, Jennifer Bruening, Kenneth Akakpo, Monica Shukla, et al.multiplex immunostaining was performed using an Opal Polaris 7-Color Automation IHC Kit (Akoya Biosciences, NEL871001KT), as recommended by the manufacturer. All multiplex immunofluorescence slides were scanned on an Akoya

(17) Tumor‐Associated Glycan Exploits Adenosine Receptor 2A Signaling to Facilitate Immune EvasionAdvanced ScienceJune 18, 2025Jing‐Yan Cheng, Hsiu‐Hui Tsai, Jung‐Tung Hung, Tsai‐Hsien Hung, Chun‐Cheng Lin, Chien‐Wei Lee, Zi‐Chi Lo, Jing‐Rong Huang, Shih‐Pin Chiou, Yenlin Huang, et al.Biosystems, Marlborough, MA). Tyramide signal amplification (TSA‐dye 520, Opal 6‐Plex Manual Detection Kit, NEL871001KT, Akoya Biosystems) was then utilized, and the slides were heated again to strip the antibodies. This

(18) Tebentafusp elicits on-target cutaneous immune responses driven by cytotoxic T-cells in uveal melanoma patientsJournal of Clinical InvestigationJune 16, 2025Ramon Staeger, Aizhan Tastanova, Adhideb Ghosh, Nicola Winkelbeiner, Prachi Shukla, Isabel Kolm, Patrick Turko, Adel Benlahrech, Jane Harper, Anna Broomfield, et al.for mean cellular staining intensity. mIHC For mIHC, Opal technology was used (Akoya Biosciences, NEL871001KT). The following primary antibodies were used: anti-CD8 (Abcam, ab4055), anti-CD68 (Abcam, ab213363),

(19) A Phase II Study of Tislelizumab as Neoadjuvant Treatment for Cisplatin-Ineligible High Risk Upper Tract Urothelial CarcinomaJournal of UrologyJune 1, 2025Jiwei Huang, Xingyun Cai, Cheoklong Ng, Yuansheng Luo, Qiong Chen, Zaoyu Wang, Keying Qiao, Wen Kong, Jin Zhang, Yonghui Chen, et al.immunofluorescence staining was performed using the Akoya OPAL Polaris 7-Color Automation IHC Kit (NEL871001KT) to assess tumor-infiltrating immune cells. Formalin-fixed paraffin-embedded slides were deparaffinized

(20) Distribution characteristics and prognostic value of TIM-1 in patients with lung adenocarcinomaFrontiers in ImmunologyMay 30, 2025Junxu Wen, Wenhua Yun, Yu Chen, Xiaoyan Yin, Wenxing Cui, Minghao Yu, Xiangjiao MengMultiplex immunofluorescence staining was performed using the Akoya Opal Kit (Akoya Biosciences, USA; NEL871001KT) in the complete histological sections. Each slide was incubated overnight at 55°C, followed by dewaxing

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Opal 6-Plex Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Automated Detection Kit) from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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Opal 6-Plex Detection Kit - for Whole Slide Imaging (formerly Opal Polaris 7 Color IHC Automated Detection Kit) from Akoya Biosciences, a Quanterix company

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