Opal 3-Plex Anti-Rb Manual Detection Kit (formerly Opal 4-Color Anti-Rabbit Manual IHC Kit) NEL840001KT from Akoya Biosciences, a Quanterix company

Product Specs
ItemOpal 3-Plex Anti-Rb Manual Detection Kit (formerly Opal 4-Color Anti-Rabbit Manual IHC Kit)
CompanyAkoya Biosciences, a Quanterix company
Price $1,152.13Supplier Page View Company Product Page
Catalog NumberNEL840001KT
Quantity1
ApplicationsMultiplex IHC
ReactivityMouse

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Akoya Biosciences, a Quanterix company

900 Middlesex Turnpike

Building 1

Billerica, MA 01821

United States

Phone: +1 (617) 765-7943

Email: sales@quanterix.com

Company Profile

Website: quanterix.com

(1) Local M1 Macrophage Reprogramming with Gluconic Acid-Coated Selenium NanoparticlesInt J NanomedicineDecember 3, 2025Zi-Xian Liao, Da-Liang Ou, Chia-Lang Hsu, Lin-Ni Lu, Cheng-Han Wen, Lin Lu, Chun-Lun Chiu, Pan-Chyr Yang, S.-Ja TsengOpal 7-Color Manual IHC Kit and the anti-Rabbit Manual IHC Kit (Akoya Biosciences, NEL810001KT and NEL840001KT) to detect specific immune cell markers in FFPE sections. Opal dyes were assigned as follows: Opal620

(2) Mitochondrial biogenesis modulation by silicon-stimulated mesenchymal stem cells-derived extracellular vesicles drives angio- and lymphangiogenesis in chronic wound healingMater Today BioDecember 3, 2025Min-Hua Yu, Yen-Hong Lin, En-Wei Liu, Tai-Yi Hsu-Jiang, Der-Yang Cho, Jian-Jr Lee, Ming-You Shiewere incubated overnight at 4 °C. The Opal 3-Plex Anti-Rb manual detection kit (Akoya Biosciences, #NEL840001KT) was used for fluorescent labeling in accordance with the manufacturer’s instructions, with sequential

(3) Establishment and characterization of an orthotopic implanted lung cancer model to mimic human tumor structure, microenvironment, and metastatic spreadTranslational Lung Cancer ResearchNovember 30, 2025Beñat Picabea, Daniel Orive, Covadonga Rodríguez, Miren Mailharin, María Sangüesa, Maeva Houry, Andrea Arricibita, Mirari Echepare, Ane Álava, Cristina Viu-Idocin, et al.staining was performed according to the manufacturer’s protocol using murine-specific reagents (Akoya, CAT#NEL840001KT), including additional opal fluorophores for enhanced target resolution. Antigen retrieval was performed

(4) Integrative multi-omics reveals a regulatory and exhausted T-cell landscape in CLL and identifies galectin-9 as an immunotherapy targetNat CommunAugust 7, 2025L. Llaó-Cid, Jkl Wong, I. Fernandez Botana, Y. Paul, M. Wierz, L-M Pilger, A. Floerchinger, C L Tan, S. Gonder, G. Pagano, et al.and appropriate antibody concentrations were optimized using the Opal 5-color kit (Akoya Biosciences, NEL840001KT). Epitope stability was assessed to determine the order of each antibody in sequence, and antigen stripping

(5) Suppression of Inflammation by Si Miao San in Experimental Rheumatoid Arthritis Through Modulation of the AKT/ROS/Autophagy AxisJ Inflamm ResJuly 17, 2025Honglin Zhang, Haixu Jiang, Qiuzhu Wei, Jie Xu, Zihan Zhao, Yuhe Sun, Qingyi Luarea. Immunofluorescence Assays (IFAs) of Tissue Sections An opal 3-plex anti-Rb manual detection kit (Cat#: NEL840001KT, Akoya Biosciences, Co., Ltd, Delaware, USA) was used to simultaneously detect the expression of target

(6) Temporal and cell-specific changes to cellular iron sequestration and lipid peroxidation in a murine model of neonatal hypoxic-ischemic brain injurybioRxivJuly 3, 2025Joseph Vithayathil, Anjali Shankar, Ginger L Milne, Frances E Jensen, Delia M Talos, Joshua L Dunaieftemperature for 2 hours. For Gpx4 staining, Akoya Opal tyramide fluorescent signal amplification kit (NEL840001KT) was used in order to improve antibody signal. Slides then washed and mounted using Fluoromount with

(7) Hydrogen sulfide aggravates neutrophil infiltration, vascular remodeling and elastase-induced abdominal aortic aneurysm in male miceCommun Med (Lond)July 1, 2025Clémence Bechelli, Diane Macabrey, Florian Caloz, Severine Urfer, Martine Lambelet, Florent Allagnat, Sébastien Dégliseimaging of mouse FFPE tissue was performed using the Opal 3-Plex Anti-Rb Manual Detection Kit (Cat: NEL840001KT, Akoya Biosciences), according to the manufacturer’s instructions using Opal 570 and Opal 690 secondary

(8) Laboratory mice engrafted with natural gut microbiota possess a wildling-like phenotypeNat CommunJune 12, 2025Solveig Runge, Silvia von Zedtwitz, Alexander M. Maucher, Philipp Bruno, Lisa Osbelt, Bei Zhao, Anne M. Gernand, Till R. Lesker, Katja Gräwe, Manuel Rogg, et al.cooking in citrate buffer (pH 6) for 5 min. Opal 4-Color Anti-Rabbit Manual IHC Kit (Akoya Biosciences, NEL840001KT) was used for staining of CD45 (Abcam, ab10558, dilution 1:200, incubation 90 min at RT), CD4 (Abcam,

(9) Reactivation of CTLA4-expressing T cells accelerates resolution of lung fibrosis in a humanized mouse modelJ Clin InvestMay 15, 2025Santosh Yadav, Muralidharan Anbalagan, Shamima Khatun, Devadharshini Prabhakaran, Yasuka Matsunaga, Justin Matsunaga, James B. McLachlan, Joseph A. Lasky, Jay Kolls, Victor J. Thannickalwas performed as described above. The Opal 3-Plex Anti-Rb Manual Detection Kit (Akoya Biosciences, NEL840001KT) was used for multiplexing. The following antibodies were used: pro-SPC (abcam, ab90716, 1:500) and

(10) Sex-specific impact of B cell-derived IL-10 on tuberculosis resistanceFront ImmunolApril 7, 2025David Hertz, Sebastian Marwitz, Lars Eggers, Linda von Borstel, Gishnu Harikumar Parvathy, Jochen Behrends, Danny D. Jonigk, Rudolf A. Manz, Torsten Goldmann, Bianca E. SchneiderTween-20) and subsequently forwarded to mIF staining (25). The Opal 3-Plex Anti-Rb Manual Detection Kit (NEL840001KT, Akoya Biosciences, Marlborough, USA) was used with additional fluorophores as indicated in Supplementary

(11) Polymeric nanocapsules loaded with poly(I:C) and resiquimod to reprogram tumor-associated macrophages for the treatment of solid tumorsFrontiers in ImmunologyJanuary 8, 2024Clément Anfray, Carmen Fernández Varela, Aldo Ummarino, Akihiro Maeda, Marina Sironi, Sara Gandoy, Jose Brea, María Isabel Loza, Sergio León, Alfonso Calvo, et al.Biosciences). Multiplexed tumor immunophenotyping For multispectral immunophenotyping, the murine-specific kit from Akoya (NEL840001KT) was used as previously described (45), with additional markers that were evaluated in two separate

(12) Nano-modified viruses prime the tumor microenvironment and promote the photodynamic virotherapy in liver cancerJournal of biomedical scienceJanuary 2, 2024Ou DL, Liao ZX, Kempson IM, Li L, Yang PC, Tseng SJ.the Opal 7-Color Manual IHC Kit and anti-Rabbit Manual IHC Kit (Akoya Biosciences, NEL810001KT and NEL840001KT), were utilized for the detection of specific markers on immune cells in the FFPE sections. Opal620

(13) Presence of Activated (Phosphorylated) STAT3 in Radiation Necrosis Following Stereotactic Radiosurgery for Brain MetastasesInternational Journal of Molecular SciencesSeptember 18, 2023Paola Anna Jablonska, Nuria Galán, Jennifer Barranco, Sergio Leon, Ramón Robledano, José Ignacio Echeveste, Alfonso Calvo, Javier Aristu, Diego Serranocontrols. For multiplex, kits from Akoya Biosciences were used according to the manufacturer’s guidelines (NEL840001KT; OP7TL4001KT). Autofluorescence and positive control samples were included for validation purposes.

(14) Tumor Immunophenotyping‐Derived Signature Identifies Prognosis and Neoadjuvant Immunotherapeutic Responsiveness in Gastric CancerAdvanced ScienceMay 1, 2023Jia‐Bin Wang, Qing‐Zhu Qiu, Qiao‐Ling Zheng, Ya‐Jun Zhao, Yu Xu, Tao Zhang, Shuan‐Hu Wang, Quan Wang, Qin‐Wen Jin, Yin‐Hua Ye, et al.T cells (Teffs; GZMB/CD8) were characterized separately in two separate panels using an Opal 4‐color Kit (NEL840001KT, Akoya Bioscience, USA). Labeling of multi‐cytokeratin using panCK (ab7753, Abcam, UK) in all panels

(15) Targeting the extra domain A of fibronectin for cancer therapy with CAR-T cellsJournal for ImmunoTherapy of CancerAugust 2, 2022Celia Martín-Otal et al.scFv F8 and Collagen IV (ab6586, Abcam). Samples were stained using an Opal 4-color anti Rabbit Kit (NEL840001KT, Akoya Biosciences) following manufacturer’s instructions. Samples were counterstained using DAPI and

(16) A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapyRegenerative therapyMarch 1, 2022Mitsuboshi S, Homma J, Sekine H, Takagi R, Shimizu T, Kanzaki M.HRP (1:5 dilution) and either the Opal 520 (1:100 dilution) or Opal 570 (1:100 dilution) fluorophore (NEL840001KT; Akoya Biosciences Inc., Marlborough, MA, USA) at 20–30 °C for 10 min. Subsequently, microwave treatment

(17) Two alternative cell line models for the study of multiorganic metastasis and immunotherapy in Lung Squamous Cell CarcinomaDisease Models & MechanismsJanuary 1, 2022Karmele Valencia et al.immunodetection of CD4, CD8, FOXP3, F4/80 and DAPI (for nuclear staining) by using the murine-specific kit (NEL840001KT) from Akoya Biosciences (Marlborough, MA, USA) following the manufacturer's instructions. Extra Alexa

(18) Intratumoral combination therapy with poly(I:C) and resiquimod synergistically triggers tumor-associated macrophages for effective systemic antitumoral immunityJournal for immunotherapy of cancerSeptember 1, 2021Anfray C, Mainini F, Digifico E, Maeda A, Sironi M, Erreni M, Anselmo A, Ummarino A, Gandoy S, Expósito F, et al.fixed in Bouin’s solution. Multiplexed tumor immunophenotyping The murine-specific kit from Akoya (NEL840001KT) was used following the manufacturer’s instructions with additional markers. The kit includes the Alexa

(19) SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferationJournal for immunotherapy of cancerMarch 1, 2021Redin E, Garmendia I, Lozano T, Serrano D, Senent Y, Redrado M, Villalba M, De Andrea CE, Exposito F, Ajona D, et al.recommendation. For multispectral immunophenotyping in mouse tumors, the murine-specific kit from Akoya (NEL840001KT) was used following the manufacturer’s instructions, with some modifications and additional markers.

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Opal 3-Plex Anti-Rb Manual Detection Kit (formerly Opal 4-Color Anti-Rabbit Manual IHC Kit) from Akoya Biosciences, a Quanterix company

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Akoya Biosciences, a Quanterix company

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Opal 3-Plex Anti-Rb Manual Detection Kit (formerly Opal 4-Color Anti-Rabbit Manual IHC Kit) from Akoya Biosciences, a Quanterix company

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