Fig 1: HA DNA-LNP elicits potent antigen-specific CD8+ and CD4+ T cell responses(A) Schematic of immunization regimen.(B and C) Representative FACS plots (B) and frequency (C) of IFNγ+ effector CD8+ T cells.(D) CD107a+ effector CD8+ T cells.(E) TNF-α+ effector CD8+ T cells.(F) IFNγ+ effector CD4+ T cells.(G) TNF-α+ effector CD4+ T cells.(H) IL-2+ effector CD4+ T cells.(I–K) Frequency of effector CD8+ T cells expressing IFNγ (I), CD107a (J), or TNF-α (K) after dose de-escalation.Dots represent individual animals; for (C–H), n = 10 animals per group; for (I–K), n = 5 animals per group. Data pooled from two (C–H) or one (I–K) independent experiment(s). Plots show geometric mean with geometric SD. Unpaired one-way ANOVA adjusted for multiple comparisons with Bonferroni corrections was used to compare groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.
Fig 2: Metabolic reprogramming of KCs after I/R associates with CD36-enriched signaling.(A) Principal component analysis (PCA) of non-targeted metabolomics profiling in I/R-KCs compared with sham-KCs. (B) Normalized hexosylceramide (HexCer) levels in sham and I/R KCs. (C) KEGG enrichment analysis of altered metabolic profiles in I/R-KCs compared with sham-KCs. (D) Scatterplot showing the top enriched functional metabolic pathways in I/R-KCs compared with sham-KCs based on metabolomics data. (E) Heatmap comparing metabolic pathway activity between sham-associated KCs and I/R–associated KCs based on scRNA-seq data. (F) Heatmap comparing metabolic pathway activity in KCs based on human scRNA-seq data from liver transplant patients (GSE171539). (G) Serum alanine aminotransferase (sALT) levels were quantified in I/R mice treated with control or LSKL peptide. (H) Serum IL-6 levels were quantified in I/R mice treated with control or LSKL peptide. (I) Serum myeloperoxidase (MPO) levels were quantified in I/R mice treated with control or LSKL peptide. Data represent the mean ± SEM. *P < 0.05, ****P < 0.0001, by 2-tailed, unpaired t test (B and G–I).
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