Fig 1: HA DNA-LNP elicits potent antigen-specific CD8+ and CD4+ T cell responses(A) Schematic of immunization regimen.(B and C) Representative FACS plots (B) and frequency (C) of IFNγ+ effector CD8+ T cells.(D) CD107a+ effector CD8+ T cells.(E) TNF-α+ effector CD8+ T cells.(F) IFNγ+ effector CD4+ T cells.(G) TNF-α+ effector CD4+ T cells.(H) IL-2+ effector CD4+ T cells.(I–K) Frequency of effector CD8+ T cells expressing IFNγ (I), CD107a (J), or TNF-α (K) after dose de-escalation.Dots represent individual animals; for (C–H), n = 10 animals per group; for (I–K), n = 5 animals per group. Data pooled from two (C–H) or one (I–K) independent experiment(s). Plots show geometric mean with geometric SD. Unpaired one-way ANOVA adjusted for multiple comparisons with Bonferroni corrections was used to compare groups. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001.