Fig 1: Evaluation of the osteogenic differentiation of the ADSC cell sheet combined with a β-TCP/COL-I scaffold (Group 1), scattered ADSCs combined with a β-TCP/COL-I scaffold (Group 2) and the ADSC cell sheet without a β-TCP/COL-I scaffold (Group 3). Relative (A) ALP activity and protein levels of (B) OCN and (C) OPN. Relative mRNA levels of (D) ALP, (E) OCN and (F) OPN. Values are expressed as the mean ± standard deviation (n=3). *P<0.05; **P<0.01. ADSC, adipose-derived stem cell; β-TCP/COL-I, β-tricalcium phosphate/collagen fiber; ALP, alkaline phosphatase; OCN, osteocalcin; OPN, osteopontin.