Fig 1: Tissue factor (TF) and Podoplanin (PDPN) protein levels are differently affected by treatment with ICIs in NSCLC cell lines. Culture supernatants’ analysis of TF and PDPN proteins by enzyme-linked immunosorbent assay (ELISA) (N = 3). (A) In cultures with A549 cells, TF levels were only slightly higher with Nivolumab and Ipilimumab exposure (compared to the respective controls). (B) In cultures with H292 cells, TF levels were increased in co-cultures with TCD8+ that were treated with both ICIs. (C) In cultures with PC-9 cells, treatment with Ipilimumab increased TF levels in the presence of TCD8+. (D) In cultures with A549 cells, PDPN levels were only slightly decreased in the presence of TCD8+ and the ICI (compared to monocultures). (E) With H292 cells, co-cultures with TCD8+ that were treated with ICIs showed an increase in PDPN protein. (F) In cultures with PC-9 cells, TCD8+ presence induced a small decrease in PDPN with Nivolumab, but an increase was seen with Ipilimumab. Results are shown as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Unpaired t-tests were used.