Description
The PDE11A Assay Kit is designed for identification of PDE11A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE11A to the binding agent. The key to the PDE11A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE11A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE11A for 1 hour. Second, a binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader