NEBExpress Cell-free E. coli Protein Synthesis System E5360L from New England Biolabs

Product Specs
ItemNEBExpress Cell-free E. coli Protein Synthesis System
CompanyNew England Biolabs
Price $1,830.00 Inquire
Catalog NumberE5360L
Quantity100 reactions
ApplicationsProteomics & Glycomics
Temperature-80 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) A Bait-and-Switch Strategy Links Phenotypes to Genes Coding for Polymer-Degrading Enzymes in Intact MicrobiomesMicrob BiotechnolApril 26, 2026Colleen E. Yancey, Kyle D. Brumfield, Jackson A. Buss, Rita R. Colwell, Laurence Ettwillerμg of plasmid DNA was added to an NEBExpress Cell‐free E. coli Protein Synthesis System reaction (E5360, New England Biolabs, Ipswich, MA, USA), scaled for a total reaction volume of 100 μL. Each reaction

(2) Accessible biocatalyst development by rapid in vitro semi-rational engineering (RISE) of enzymesiScienceJanuary 16, 2026András Telek, Viktor Zsolt Gaják, Gergő Dargó, Jan Mičan, David Bednář, Beáta G. Vértessy, Gábor Tasnádicommercial assays,Critical commercial assays ,, NEBExpress® Cell-free E. coli Protein Synthesis System,NEB,E5360L ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, Primers (Table S5),Microsynth,N/A ,, Recombinant

(3) Critical role of the CMGC insert sequence for tyrosine autophosphorylation in the protein kinase DYRK1BSci RepDecember 24, 2025Silvia Detro-Dassen, Katharina Schwandt, Philip Helmich, Stefan Düsterhöft, Walter Beckerconstructs was performed with the NEBExpress Cell-free Protein Synthesis System based on E. coli extracts (#E5360S, New England Biolabs, Ipswich, MA, USA). Reactions were performed in a volume of 6 µL with 40 ng plasmid

(4) A Bait-and-Switch strategy links phenotypes to genes coding for Polymer-Degrading Enzymes in Intact MicrobiomesbioRxivOctober 14, 2025Colleen E. Yancey, Kyle D. Brumfield, Jackson Buss, Rita R. Colwell, Laurence Ettwiller1 μg of plasmid DNA was added to an NEBExpress Cell-free E. coli Protein Synthesis System reaction (E5360, New England Biolabs, Ipswich, MA, USA), scaled for a total reaction volume of 100 μL. Each reaction

(5) Systematic identification and characterization of eukaryotic and viral 2A peptide-bond-skipping sequencesCell reportsJuly 22, 2025Rao DM, Horton ER, Barrington CL, Briney CA, Henriksen JC, Martinez-Seidel F, Morrison EJ, Sterling EM, Provencio ED, Harris M, et al.,New England Biolabs,P8116L NEBExpress® Cell-free E. coli Protein Synthesis System,New England Biolabs,E5360L NuPAGE™ MES SDS Running Buffer (20×),Invitrogen,NP0002 NuPAGE™ Transfer Buffer (20×),Invitrogen,NP00061 Quick-Start

(6) Bacterial Shedu immune nucleases share a common enzymatic core regulated by diverse sensor domainsMolecular CellFebruary 6, 2025Yajie Gu, Huan Li, Amar Deep, Eray Enustun, Dapeng Zhang, Kevin D. Corbettassays,Critical commercial assays NEBExpress Cell-free E. coli Protein Synthesis System,New England Biolabs,E5360S "UltrAufoil R 1.2/1.3, 300 Mesh, gold cryoEM grids",Electron Microscopy Sciences,Q350AR13A Deposited

(7) Toehold switch plus signal amplification enables rapid detectionBiotechnology JournalDecember 1, 2023Kevin Morey, Tyler Thomas‐Fenderson, Al Watson, Jacob Sebesta, Christie Peebles, Claudia Gentry‐Weeksto the manufacturer’s instructions using the NEBExpress Cell-free E. coli Protein Synthesis System #E5360 (NEB, Ipswich, MA). This kit contains the T7 RNA polymerase expression system, including the necessary

(8) Initiation at AUGUG and GUGUG sequences can lead to translation of overlapping reading frames in E. coliNucleic Acids ResearchJanuary 11, 2023Maximilian P Kohl, Maria Kompatscher, Nina Clementi, Lena Holl, Matthias D Erlacherbased IVTs for luciferase reporter assays were carried out using the cell-free NEBExpress™ system (NEB, E5360L) according to the manufacturer's protocol, but scaled to a final reaction volume of 6.25 μl. 30 ng of

(9) Rapid modeling of experimental molecular kinetics with simple electronic circuits instead of with complex differential equationsFrontiers in Bioengineering and BiotechnologySeptember 28, 2022Yijie Deng, Douglas Raymond Beahm, Xinping Ran, Tanner G. Riley, Rahul Sarpeshkarcell-free system The cell-free E. coli protein synthesis system was purchased from New England BioLabs (cat# E5360). The T7 RNA Polymerase (NEB, cat# M0251S) and RNase Inhibitor (NEB, cat#M0314S) were added to the reaction

(10) A Multi-Specific DARPin Potently Neutralizes Shiga Toxin 2 via Simultaneous Modulation of Both Toxin SubunitsBioengineering (Basel, Switzerland)September 27, 2022Zeng Y, Jiang M, Robinson S, Peng Z, Chonira V, Simeon R, Tzipori S, Zhang J, Chen Z.μL of this mixture was added to 1× NEBExpress Cell-free E. coli Protein Synthesis System (NEB, Cat# E5360S) containing 3 μL of S30 extract, 6 μL of Protein Synthesis Buffer, 0.2 μL T7 RNA polymerase, and 0.2

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NEBExpress Cell-free E. coli Protein Synthesis System

NEBExpress Cell-free E. coli Protein Synthesis System from New England Biolabs

New England Biolabs

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NEBExpress Cell-free E. coli Protein Synthesis System from New England Biolabs

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