Glycerol-Glo Assay J3150 from Promega

Product Specs
ItemGlycerol-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ3150
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) A small‑molecule HDAC/PDE modulator activates human adipocyte UCP1 and resolves inflammatory signalingJ Transl MedMarch 5, 2026Mayyasa Rammah, Atilio Reyes Romero, Najeeb Halabi, Sirin Abuaqel, Andrea Guennoun, Alexander Dömling, Jingxuan Shan, Lotfi ChouchaneSigma-Aldrich, I6504) for 2 hours. Glycerol levels were measured using the Glycerol-Glo™ Assay (Promega, J3150) according to the manufacturer’s protocol. Glycerol concentrations were normalized to cell viability,

(2) Ei24 deficiency in brown adipocytes induces severe hypothermia under cold stress independent of UCP1 activityNat CommunDecember 1, 2025Su Bin Lee, Bui Thanh Thu, Tae Wook Nam, Yaechan Song, Jae Hoon Lee, Yangsik Jeong, Han-Woong Leetriacylglycerol and glycerol concentration. Measurements were conducted using Glycerol-GloTM assay kit (J3150, Promega). For evaluation of lipogenic rate, terminally differentiated adipocytes were incubated in

(3) Activation of lysosomal iron triggers ferroptosis in cancerNatureJune 1, 2025Cañeque T, Baron L, Müller S, Carmona A, Colombeau L, Versini A, Solier S, Gaillet C, Sindikubwabo F, Sampaio JL, et al.with Fento-1 (in-house, 1 or 2 µM, 24 h). Glycerol was quantified using a Glycerol-glo assay (Promega, J3150) according to the manufacturer’s protocol. In brief, the assay was performed in a 96-well plate and

(4) White adipocytes in subcutaneous fat depots require KLF15 for maintenance in preclinical modelsJournal of Clinical InvestigationJuly 1, 2024Liang Li, Brian J. FeldmanSigma-Aldrich) for 1 hour. Aliquots of medium were taken to assay for glycerol content using Glycerol-Glo (J3150, Promega) according to the manufacturer’s protocol. Glycerol content was normalized to total cellular

(5) Tumoral acidosis promotes adipose tissue depletion by fostering adipocyte lipolysisMolecular metabolismMay 1, 2024Lefevre C, Thibaut MM, Loumaye A, Thissen JP, Neyrinck AM, Navez B, Delzenne NM, Feron O, Bindels LB.in adipocyte supernatants and plasma were measured using glycerol (Sigma, MAK117 and F6428; Promega, J3150) and NEFA (Randox) assay kits according to the manufacturer's protocol. In vivo lipolytic activity was

(6) Impaired Adipocyte SLC7A10 Promotes Lipid Storage in Association With Insulin Resistance and Altered BCAA MetabolismThe Journal of Clinical Endocrinology & MetabolismAugust 18, 2023Regine Å Jersin, Divya Sri Priyanka Sri Priyanka Tallapragada, Linn Skartveit, Mona S Bjune, Maheswary Muniandy, Sindre Lee-Ødegård, Sini Heinonen, Marcus Alvarez, Kåre Inge Birkeland, Christian André André Drevon, et al.Solution was added, and data were normalized to cell number/well using Hoechst staining. For glycerol (J3150, Promega) and cholesterol (J3190, Promega) content, data were normalized to DNA content per well. Hoechst

(7) Identification of a weight loss-associated causal eQTL in MTIF3 and the effects of MTIF3 deficiency on human adipocyte functioneLifeMarch 6, 2023Huang M, Coral D, Ardalani H, Spegel P, Saadat A, Claussnitzer M, Mulder H, Franks PW, Kalamajski S.incubation, the medium was collected, and the glycerol content was measured using Glycerol-Glo Assay (J3150, Promega). Total triglyceride measurement Triglyceride-Glo Assay kit (J3161, Promega) was used to

(8) Oncostatin M promotes lipolysis in white adipocytesAdipocyteDecember 31, 2022Pim P. van Krieken, Julian Roos, Pamela Fischer-Posovszky, Stephan Wueest, Daniel Konradstimulation with vehicle control or 10 or 100 ng/ml recombinant human OSM. The Glycerol-Glo assay (Promega J3150) was used to determine free glycerol in the supernatant. Lactate dehydrogenase Lactate accumulation

(9) Regulation of Adipose Progenitor Cell Expansion in a Novel Micro-Physiological Model of Human Adipose Tissue Mimicking Fibrotic and Pro-Inflammatory MicroenvironmentsCellsSeptember 7, 2022Dani V, Bruni-Favier S, Chignon-Sicard B, Loubat A, Doglio A, Dani C.according to the manufacturer’s instructions using a Glycerol Detection Assay (Promega, Madison, WI, USA, #J3150). 2.6. RNA Extraction and Reverse Transcription Quantitative Polymerase Chain Reaction One hundred

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Glycerol-Glo Assay

Glycerol-Glo Assay from Promega

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Glycerol-Glo Assay from Promega

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