Triglyceride-Glo Assay J3160 from Promega

Product Specs
ItemTriglyceride-Glo Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberJ3160
Quantity5ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assemblyNatureFebruary 25, 2026Alyssa J. Mathiowetz, Emily S. Meymand, Güneş Parlakgül, Niek van Hilten, Emily F. Torres, Leonardo L. Artico, Kirandeep K. Deol, Mike Lange, Stephany P. Pang, Cody E. Doubravsky, et al.analysed via Clinical Analyzer (Merck & Co). TAGs were quantified by a luciferase-based assay (Promega, J3160). FPLC separation of mouse plasma Size-exclusion chromatography was performed using an AKTA FPLC (Amersham

(2) BRUCE liver-KO enhances MASLD/MASH development in the steatotic PTEN-KO background by impairing mitochondrial metabolism and activating STAT3Cell Death & DiseaseDecember 11, 2025Lixiao Che, Camille K. Stevenson, David R. Plas, Jiang Wang, Chunying Duwere quantified by a luciferase-based assay as described by the manufacturer’s instructions (Promega J3160). Primary hepatocyte isolation Primary hepatocytes were isolated from anesthetized mice by IVC perfusion

(3) Alcohol and Metabolic Stress Synergize to Dysregulate Mitochondrial Health and Lipid Metabolism; Evidence from a Hepatocyte Spheroid ModelFunction (Oxf)November 3, 2025Eden M Gallegos, Kaitlin Couvillion, Drake Darden, Keishla Rodriguez-Graciani, Patricia E Molina, Liz SimonQuantification Triglyceride content in each spheroid was quantified at endpoint using the Triglyceride-Glo™ Assay (J3160, Promega). Briefly, spheroids were washed with 1X PBS pH 7.4 before being transferred into glycerol

(4) Herbal terpenoids activate autophagy and mitophagy through modulation of bioenergetics and protect from metabolic stress, sarcopenia and epigenetic agingNat AgingOctober 1, 2025Civiletto G, Brunetti D, Lizzo G, Muller K, Jacot GE, Daskalaki I, Sizzano F, Huh M, Di Meo I, Colombo MN, et al.kit (Crystal Chem, 90080). Liver triglyceride measurements The Triglyceride-Glo Assay kit (Promega, J3160) was used to measure triglyceride levels in mouse liver homogenates. Homogenates were prepared from

(5) Loss of Nuclear TDP-43 Impairs Lipid Metabolism in Microglia-Like CellsbioRxivSeptember 21, 2025Khushbu Kabra, Dallin Dressman, Ryan Talcoff, Maedot Yidenk, Olivia M. Rifai, Benjamin N. Hoover, Neil A. Shneider, Wassim Elyaman, Estela Area-Gomez, Elizabeth M. Bradshawvalues were normalized to protein levels. Triglyceride/Glycerol Assay: Triglyceride-Glo Assay (Promega J3160) was used to measure total glycerol and free glycerol in cells and supernatants, as per the manufacturer’s

(6) Linear ubiquitination prevents lipodystrophy and obesity-associated metabolic syndromeScience advancesSeptember 19, 2025Hildebrandt X, Veli Ö, Hyoubi A, Zinngrebe J, Abdallah AT, Rodefeld J, Hoffmann A, Gardeweg L, Kaya Ö, Wagner E, et al.kits: free fatty acids (Abnova, KA1667), cholesterol (Promega, J3190), and triglycerides (Promega, J3160). Cell culture Mouse adipocytes and preadipocytes cell culture The fibroblast preadipocyte line

(7) GPX4-dependent ferroptosis sensitivity is a fitness trade-off for cell enlargementiScienceMay 16, 2025Kuan Yoow Chan, Yini Yu, Yidi Kong, Ling Cheng, Renzhi Yao, Phoebe Sha Yin Chair, Ping Wang, Rong Wang, Wan-Yang Sun, Rong-Rong He, et al.assay For measuring triglyceride levels in RPE1 cells, Triglyceride-Glo Assays was used (Promega, J3160). Briefly, RPE1 cells were plated at 0.5 × 105/mL and incubated with and without 50 nM doxorubicin in

(8) Engineered Hydrogels for Organoid Models of Human Nonalcoholic Fatty Liver DiseaseAdvanced ScienceMay 14, 2025Yueming Liu, Aidan E. Gilchrist, Patrik K. Johansson, Yuan Guan, Jaydon D. Deras, Yu‐Chung Liu, Sofia Ceva, Michelle S. Huang, Renato S. Navarro, Annika Enejder, et al.Assay Triglyceride quantification in organoids was measured using Triglyceride‐Glo assay (Promega J3160). Hydrogels were treated with equal volume of elastase (500 U mL−1; GoldBio) and hyaluronidase (2000

(9) Ninjurin 2 Modulates Tumorigenesis, Inflammation, and Metabolism via PyroptosisThe American Journal of PathologyMay 1, 2025Jin Zhang, Xiangmudong Kong, Hee Jung Yang, Weici Zhang, Mingyi Chen, Xinbin ChenCholesterol/Cholesterol Ester-Glo Assay kit (catalog number J3190; Promega) and Triglyceride-Glo Assay kit (catalog number J3160; Promega) were used according to the user's manual. Briefly, mouse sera were first incubated with cholesterol/triglyceride

(10) Perivascular Adipocytes' Adipogenesis Is Defined by Their Anatomical Location in the Descending Thoracic AortaCellsApril 11, 2025G. Andres Contreras, C. Javier Rendon, Alyssa Shadowens, Miguel Chirivi, David Salcedo-Tacuma, D. Adam Lauver, Stephanie W. Wattsto assess triglyceride content using the Triglyceride-Glo kit (Promega, Madison, WI, USA, Cat. No. J3161) and adiponectin levels using the Rat Total Adiponectin/Acrp30 ELISA kit (R&D Systems, Minneapolis,

(11) Cinnabarinic acid protects against metabolic dysfunction-associated steatohepatitis by activating aryl hydrocarbon receptor-dependent AMPK signalingAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyApril 1, 2025Nikhil Y. Patil, Iulia Rus, Felix Ampadu, Hassan M. Abu Shukair, Sarah Bonvicino, Richard S. Brush, Elena Eaton, Martin-Paul Agbaga, Tae Gyu Oh, Jacob E. Friedman, et al.manner. Measurement of triglycerides, cholesterol, and free fatty acids. A bioluminometric Triglyceride-Glo™ (#J3160, Promega, Madison, WI) and cholesterol/cholesterol-ester Glo™ assays (#J3190, Promega) were used to

(12) TAp63γ is the primary isoform of TP63 for tumor suppression but not developmentCell Death DiscovFebruary 6, 2025Xinbin Chen, Wenqiang Sun, Xiangmudong Kong, Xin Ming, Yanhong Zhang, Wensheng Yan, Shakur Mohibi, Mingyi Chen, Keith Mitchell, Jin ZhangCEs Cholesterol/Cholesterol Ester-Glo Assay kit (Cat# J3190, Promega) and Triglyceride-Glo Assay kit (Cat# J3160, Promega) from Promega were used to measure Cholesterol and TAGs according to the user’s manual. Briefly,

(13) Divergent roles of m6A in orchestrating brown and white adipocyte transcriptomes and systemic metabolismNat CommunJanuary 9, 2025Ling Xiao, Dario F. De Jesus, Cheng-Wei Ju, Jiang-Bo Wei, Jiang Hu, Ava DiStefano-Forti, Valeria Salerno Gonzales, Tadataka Tsuji, Siying Wei, Matthias Blüher, et al.measurements For plasma samples, FFAs (Abcam ab65341) and triglyceride (TG) (Promega Triglyceride-Glo™ J3160) concentrations were measured using according to the manufacturer’s instructions.To measure the TG levels

(14) Tau is required for glial lipid droplet formation and resistance to neuronal oxidative stressNature NeuroscienceOctober 1, 2024Lindsey D. Goodman, Isha Ralhan, Xin Li, Shenzhao Lu, Matthew J. Moulton, Ye-Jin Park, Pinghan Zhao, Oguz Kanca, Ziyaneh S. Ghaderpour Ghaderpour Taleghani, Julie Jacquemyn, et al.quantification. Triglyceride assay on fly heads The manufacturer’s protocol for the Triglyceride-Glo Assay (Promega, no. J3160) was followed using 10 μl per head for the initial lysis step. A total of 25 frozen fly heads were used

(15) m(6)A mRNA methylation in brown fat regulates systemic insulin sensitivity via an inter-organ prostaglandin signaling axis independent of UCP1Cell metabolismOctober 1, 2024Xiao L, De Jesus DF, Ju CW, Wei JB, Hu J, DiStefano-Forti A, Tsuji T, Cero C, Männistö V, Manninen SM, et al.strip),Cayman Chemical,516011 Magnetic mRNA Isolation Kit,NEB,S1550S Triglyceride-Glo Assay,Promega,J3160 Free Fatty Acid Assay Kit - Quantification,Abcam,ab65341 ,, Deposited data,Deposited data,Deposited

(16) CLCC1 promotes hepatic neutral lipid flux and nuclear pore complex assemblybioRxivSeptember 13, 2024Alyssa J. Mathiowetz, Emily S. Meymand, Kirandeep K. Deol, Güneş Parlakgül, Mike Lange, Stephany P. Pang, Melissa A. Roberts, Emily F. Torres, Danielle M. Jorgens, Reena Zalpuri, et al.analyzed via Clinical Analyzer. Triglycerides were quantified by a luciferase-based assay (Promega, J3160) Flow cytometry Cells were washed 2x in DPBS, dissociated using TrypLE Express (Gibco, 12605010),

(17) LncRNA SOX9-AS1 triggers a transcriptional program involved in lipid metabolic reprogramming, cell migration and invasion in triple-negative breast cancerScientific ReportsJanuary 17, 2024Mireya Cisneros-Villanueva, Marco Antonio Fonseca-Montaño, Magdalena Ríos-Romero, César López-Camarillo, Silvia Jiménez-Morales, Elizabeth Langley, Alan Sajid Rosette-Rueda, Alberto Cedro-Tanda, Daniel Hernández-Sotelo, Alfredo Hidalgo-MirandaHCC1187 and MDA-MB-468 cell lysates were detected by luminescence using Triglyceride-Glo Assay (Cat. J3160, Promega, USA), according to the manufacturer’s instructions. Briefly, 10,000 transfected cells were

(18) Glycine homeostasis requires reverse SHMT fluxCell MetabolismJanuary 2, 2024Matthew J. McBride, Craig J. Hunter, Zhaoyue Zhang, Tara TeSlaa, Xincheng Xu, Gregory S. Ducker, Joshua D. Rabinowitzwith sucrose) for 12 weeks. Liver triglyceride levels were quantified by Triglyceride-Glo Assay kit (J3160, Promega). Immunoblot analysis Protein lysates were prepared from liver samples by 2% SDS whole cell lysis

(19) Single‐cell transcriptomics stratifies organoid models of metabolic dysfunction‐associated steatotic liver diseaseThe EMBO JournalDecember 11, 2023Anja Hess, Stefan D Gentile, Amel Ben Saad, Raza‐Ur Rahman, Tim Habboub, Daniel S Pratt, Alan C Mullenbuffer. Triglyceride quantification was performed using the enzymatic assay Triglyceride‐GloTM Assay (J3160, Promega) following the manufacturer's instructions. Bodipy staining After being cultured with OA/PA/TGF‐ꞵ1

(20) Nipsnap1—A regulatory factor required for long-term maintenance of non-shivering thermogenesisMolecular MetabolismSeptember 1, 2023Yang Liu, Yue Qu, Chloe Cheng, Pei-Yin Tsai, Kaydine Edwards, Siwen Xue, Supriya Pandit, Sakura Eguchi, Navneet Sanghera, Joeva J. Barrowas described in Method 2.2. Total triglycerides were measured using Triglyceride-Glo™ Assay (Promega J3160) following the manufacturer's instructions. 2.12 De novo lipogenesis stable isotope tracing of free

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Triglyceride-Glo Assay

Triglyceride-Glo Assay from Promega

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Triglyceride-Glo Assay from Promega

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