Fig 1: Assessment of the function of autophagy in the CIA group. The rats were divided randomly into six groups: (sham group, CIA group, NC-KD group, KD-NLRP3 group, OE-NC group, and OE-NLRP3 group, with 8 rats in each group). (A) Western blot and quantitative analysis of LC3, P62, and Becline1 expression level. (B) Serum levels of P62, Beclin1, and LC3. (C) Immunohistochemistry images and quantitative analysis of P62 in knee joint synovium of the CIA rats (scale bar: 100 μm). The red arrow shows the injury region. Data are presented as the mean ± SD.*, P < 0.05; **, P < 0.01. ns: not significant.
Fig 2: The expression of BECN1 and MAP1LC3B genes in the MCF-7 and MDA-MB-231 breast cancer cells after 24 h of incubation with Les-6287 and the doxorubicin at 1 µM concentration: BECN1 expression in MCF-7 (A) and MDA-MB-231 (B) cells; MAP1LC3B expression in MCF-7 (C) and MDA-MB-231 (D) cells. Data are presented as M ± SD from three independent experiments performed in duplicate. *** p < 0.001; **** p < 0.0001; ns—non-significant changes.
Supplier Page from Abcam for Human Beclin-1 ELISA Kit