Fig 2: Animal experiments validate the expression of key lipid metabolism‐autophagy targets in atherosclerosis. A and B. Representative images and quantification of Oil Red O‐stained en face aorta. C and D. Representative images of Oil Red O staining of the aortic roots, and the related quantitative analysis. E‐G. The mRNA expression levels of three key lipid metabolism‐autophagy genes in aortic tissue. H and I. Representative immunoblots and expression quantification of the three key lipid metabolism‐autophagy proteins. J and K. IHC staining of three key proteins in the aortic root from control and AS groups. L. TNFSF10 levels in the serum of control and AS group mice. Comparisons between groups were performed using an unpaired t‐test or the Mann‐Whitney test.