Fig 2: PLA and AgRP increase lean mass content, improve in vivo muscle function and femoral bone strength in Ctns −/− mice. Twelve‐month‐old Ctns −/− and WT mice were administered with PLA, AgRP, or vehicle, respectively. Figures 3 and 4 describe detailed study procedures. Lean mass content of individual mice was measured using quantitative magnetic resonance analysis. In vivo muscle function (grip strength and rotarod) was assessed. Femoral shaft biomechanical tests were performed. Femur protein contents of JAK2 and STAT3 were measured. Data are expressed as mean ± SEM. Results were analysed and expressed as in Figures 3 and 4. Ns, not significant, *P < 0.05, **P < 0.01.