Fig 1: Suppressive effects of ExohTERT-HPNE on PC cells and PSCs through delivering mature miR-485-3p into the corresponding cells. (A) Transwell migration and invasion assays of BxPC-3 and PANC-1 cells treated with or without ExohTERT-HPNE (bar: 100 µm). Cells were imaged at 200 × magnification in five random fields and counted using ImageJ software. (B) Transwell migration assay of PSCs treated with or without ExohTERT-HPNE (bar: 100 µm). (C) FN1, COLIA1, and FAP levels in cell supernatant of PSCs treated with or without ExohTERT-HPNE assessed by ELISA. (D) Detection of miR-485-3p in exosomes of hTERT-HPNE, PSCs, and PC cells, as well as in the corresponding parental cells using RT-PCR. MiR-485-3p was not detected in ExoPSC, ExoBxPC-3, and ExoMIAPaCa-2. U6 served as endogenous reference. (E) Mature miR-485-3p and its precursors (pre-miR-485-3p, pri-miR-485-3p) of PSCs and PC cells were detected by RT-PCR. U6 and GAPDH, respectively, served as endogenous reference of miRNA and mRNA. *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; miRNAs: microRNAs; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells; RT-PCR: Real-time PCR; WT: Wild type.
Fig 2: MiR-485-3p regulated PC cell migration and Invasion. (A, B) miR-485-3p was overexpressed and silenced In BxPC-3 and PANC-1 cells. (C, D) Transwell migration and Invasion assays in BxPC-3 and PANC-1 cells with overexpression or silencing of miR-485-3p (bar: 100 µm). The migrated or invaded cells were imaged at 200 × magnification in five random fields and counted using ImageJ software. (E) MiR-485-3p was overexpressed and silenced in PSCs. (F) FN1, COLIA1, and FAP levels in cell supernatant of PSCs transfected with miR-485-3p mimic or Inh-miR-485-3p were assessed by ELISA. *P < 0.05, **P < 0.01, ***P < 0.001,****P < 0.0001. COLIA1: Collagen type alpha 1 chain; ELISA: Enzyme linked immunosorbent assay; FAP: Fibroblast activation protein alpha; FN1: Fibronectin 1; PC: Pancreatic cancer; PSCs: Pancreatic stellate cells.
Supplier Page from Wuhan Fine Biotech Co., Ltd. for Human FAPα(Fibroblast Activation Protein Alpha) ELISA Kit