NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads E7765L from New England Biolabs

Product Specs
ItemNEBNextUltraII Directional RNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $3,596.00 Inquire
Catalog NumberE7765L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Overnutrition in mice impairs thyroid hormone biosynthesis and utilization, causing hypothyroidism, despite remarkable thyroidal adaptationsJ Clin InvestApril 15, 2026Jessica Rampy, Alejandra Paola Torres-Manzo, Kendra Hoffsmith, Matthew A. Loberg, Quanhu Sheng, Federico Salas-Lucia, Antonio C. Bianco, Rafael Arrojo E Drigo, Huiying Wang, Vivian L. Weiss, et al.mouse thyroids, cDNA libraries were constructed using the NEBNext Ultra II RNA Library Prep Kit (NEB #E7765L), and next-generation sequencing (Illumina NovaSeq 6000) was performed, according to the manufacturers’

(2) Activation of the protective arm of renin-angiotensin system enhances mitochondrial turnover improving respiration and decreasing integrated stress response in a human Complex III deficiency modelbioRxivMarch 27, 2026Lucía Fernández-del-Río, Andrea Eastes, David Rincón Fernández Rincón Fernández Pacheco, Nikole Scillitani, Jasmine Garza, Matthew Dugan, Matheus Pinto Pinto de Oliveira, Pradnya Kadam, Iman Gauhar, Karel Erion, et al.sequencing library was constructed by using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, E7765L) following the manufacturer’s instructions. End repair, A-tailing, and adapter ligation were performed

(3) DCPS modulates TDP-43-linked neurodegeneration through P-body-mediated RNA decayNeuronMarch 9, 2026Yingzhi Ye, Zhe Zhang, Yu Xiao, Chengzhang Zhu, Noelle Wright, Julie Asbury, Yongxin Huang, Weiren Wang, Laura Gomez-Isaza, Juan C. Troncoso, et al.kit,Navinci,Cat #NT.MR.100 NEBNext Ultra II Directional RNA Library Prep Kit,New England Biolabs,Cat #E7765L ,, Deposited data,Deposited data,Deposited data ,, ARTR-seq data from human i3Neurons,This paper,GEO:

(4) Employing Tribe to Study RNA Interactions of Ataxin-2 in Drosophila S2 CellsBIO-PROTOCOLMarch 5, 2026Shiva Kumar, Omkar Koppaka, Khushboo Agrawal, Baskar BakthavachaluE7490L) g. NEBNext Ultra II Directional RNA Library Prep kit (New England Biolabs, catalog number: E7765L) h. Unique Dual Index UMI Adaptors RNA (New England Biolabs, catalog number: E7416L) i. NovaSeq 6000

(5) Mutant GNAS drives a pyloric metaplasia with tumor suppressive glycans in intraductal papillary mucinous neoplasiaCell ReportsDecember 23, 2025Vincent Quoc-Huy Quoc-Huy Trinh, Katherine E. Ankenbauer, Sabrina M. Torbit, Christopher P. Taranto, Jiayue Liu, Maelle Batardiere, Bhoj Kumar, H. Carlo Maurer, Frank Revetta, Zhengyi Chen, et al.E7490), and the sequencing library was constructed using the NEBNext Ultra II RNA Library Prep Kit (E7765L) following the manufacturer’s instructions. End repair, A-tailing, and adapter ligation was performed

(6) High-throughput in silico screen uncovers key regulators of 3D genome architecturebioRxivDecember 11, 2025Jiangshan Bai, Qingji Lyu, Jimin Tan, Robbyn Issner, Bailey Tischer, Hanqing Liu, Viraat Goel, Xinyu Ling, Bradley E. Bernstein, Aristotelis Tsirigos, et al.RNA sequencing libraries were constructed using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, E7765L) following the rRNA depletion workflow. Computational analysis: In silico genetic screen with C.Origami We

(7) Mapping the Subtype-Specific PARP1 ADP-ribosylated Proteome in Breast Cancer CellsbioRxivOctober 5, 2025Sneh Koul, Minjung Kwon, Poulami Tapadar, Yangyang Dai, Tulip Nandu, Dan Huang, Cristel V. Camacho, W. Lee Krausaccording to the manufacturer’s instructions. The NEBNext Ultra II Directional RNA Library Prep (NEB, E7765L) in conjunction with Poly(A) mRNA Magnetic Isolation Module kit (NEB, E7490L) was then used to prepare

(8) Chronic activation of a key exercise signal transducer, CaMKII, drives skeletal muscle aging and sarcopeniabioRxivAugust 2, 2025Michael R. Bene, Tae Chung, William A. Fountain, Giovanni Rosales-Soto, Erick Hernández-Ochoa, Corina Antonescu, Liliana Florea, Seeun J. Jeong, Anne Le, Qian-Li Xue, et al.RNA library preparation with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England Biolabs, E7765L). Each library was uniquely barcoded using the NEBNext Multiplex Oligos for Illumina (96 Unique Dual

(9) Asrij/OCIAD1 depletion reduces inflammatory microglial activation and ameliorates Aβ pathology in an Alzheimer's disease mouse modelJ NeuroinflammationMarch 20, 2025Prathamesh Dongre, Madhu Ramesh, Thimmaiah Govindaraju, Maneesha S. InamdarNEBNext Ultra II Directional RNA Library Prep (stranded) kit with sample purification beads (NEB, #E7765L). cDNA libraries were sequenced on the NovaSeq 6000 platform (Illumina) with 2 × 100 bp sequencing read

(10) Evaluating the pathogenic and immunological effects of ds-GFP as a control in in vivo RNA interference studies of Schistosoma japonicumParasiteFebruary 26, 2025Lei Xiu, Xiaoling Wang, Shaoyun Cheng, Wanling Liu, Lu Wang, Jiaqi Li, Jinrui Zhang, Yaping Xuan, Wei HuDirectional RNA Library Prep with Sample Purification Beads (New England Biolabs, Ipswich, MA, USA; Cat #: E7765L), according to the manufacturer’s protocol. After cluster generation on a cBot Cluster Generation System

(11) Rhodoquinone carries electrons in the mammalian electron transport chainCellFebruary 20, 2025Jonathan Valeros, Madison Jerome, Tenzin Tseyang, Paula Vo, Thang Do, Diana Fajardo Fajardo Palomino, Nils Grotehans, Manisha Kunala, Alexandra E. Jerrett, Nicolai R. Hathiramani, et al.Chloroform,Sigma-Aldrich,Cat#366927 ,, Kits,, NEBNext Ultra II Directional RNA Library Prep Kit,New England Biolabs,Cat# E7765L NEBNext Multiplex Oligos for Illumina,New England Biolabs,cat# E6440S NEBNext Library Quant Kit for

(12) Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structureNat CommunFebruary 19, 2025Kodai Yamaura, Naomichi Takemata, Masashi Kariya, Ayami Osaka, Sonoko Ishino, Masataka Yamauchi, Tomonori Tamura, Itaru Hamachi, Shoji Takada, Yoshizumi Ishino, et al.library construction with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7765L). Reaction was performed according to the manufacturer’s instructions. ChIP-seq ChIP-seq was carried

(13) Viral oncogene EBNALP regulates YY1 DNA binding and alters host 3D genome organizationEMBO ReportsJanuary 2, 2025Chong Wang, Merrin Manlong Leong, Weiyue Ding, Yohei Narita, Xiang Liu, Hongbo Wang, Stefanie P T Yiu, Jessica Lee, Katelyn R S Zhao, Amy Cui, et al.kit,NEB,E7645S NEBNext® Ultra™ II Directional RNA Library Prep with Sample Purification Beads,NEB,E7765L RNeasy Mini kit,Qiagen,74004 RosetteSep Human B Cell Enrichment Cocktail and,STEMCELL,15064 EasySep

(14) A constitutive heterochromatic region shapes genome organization and impacts gene expression in Neurospora crassaBMC GenomicsDecember 20, 2024Andrew T. Reckard, Abhishek Pandeya, Jacob M. Voris, Carlos G. Gonzalez Cruz, Oluwatosin Oluwadare, Andrew D. Klockogenerated with the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, cat# E7760L and E7765L) per the manufacturer’s protocols; quadruplicate polyA mRNA-seq replicate libraries were constructed

(15) Disruption of nuclear speckle integrity dysregulates RNA splicing in C9ORF72-FTD/ALSNeuronOctober 23, 2024Rong Wu, Yingzhi Ye, Daoyuan Dong, Zhe Zhang, Shaopeng Wang, Yini Li, Noelle Wright, Javier Redding-Ochoa, Koping Chang, Shaohai Xu, et al.analysis RNA-seq libraries were prepared using NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Cat. No. E7765L) with NEBNext Poly(A) mRNA Magnetic isolation Module (NEB, Cat. No. E7490L) according to the manufacturer’s

(16) RBN-2397, a PARP7 Inhibitor, Synergizes with Paclitaxel to Inhibit Proliferation and Migration of Ovarian Cancer CellsbioRxivSeptember 10, 2024Alexandra N. Spirtos, Marwa W. Aljardali, Sridevi Challa, Sneh Koul, Jayanthi S. Lea, W. Lee Kraus, Cristel V. Camachoaccording to the manufacturer’s instructions. The NEBNext Ultra II Directional RNA Library Prep (NEB, E7765L) in conjunction with Poly(A) mRNA Magnetic Isolation Module kit (NEB, E7490L) was then used to prepare

(17) Pan-transcriptome reveals a large accessory genome contribution to gene expression variation in yeastNature GeneticsJune 1, 2024Élodie Caudal, Victor Loegler, Fabien Dutreux, Nikolaos Vakirlis, Élie Teyssonnière, Claudia Caradec, Anne Friedrich, Jing Hou, Joseph Schachererwith the NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (cat. no. E7765L, New England Biolabs) in 96-well plates. We used 5 µl purified mRNA for library preparation, corresponding

(18) DDR2 signaling and mechanosensing orchestrate neuroblastoma cell fate through different transcriptome mechanismsFEBS Open BioMay 1, 2024Theadora Vessella, Steven Xiang, Cong Xiao, Madelyn Stilwell, Jaidyn Fok, Jason Shohet, Esteban Rozen, H. Susan Zhou, Qi WenIpswitch, MA, USA, Cat. No. E7400L), NEBNext Ultra™ II Directional RNA Library Prep kits (NEB, Cat. No. E7765L) and IDT xGEN UDI primers (Integrated DNA Technologies, Coralville, IA, USA, Cat. No. 10008052) according

(19) Diallel panel reveals a significant impact of low-frequency genetic variants on gene expression variation in yeastMolecular Systems BiologyFebruary 14, 2024Andreas Tsouris, Gauthier Brach, Anne Friedrich, Jing Hou, Joseph Schacherersequencing libraries were prepared with the NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, #E7765L) and using the manufacturer’s protocol. The concentration of cDNA in each library was quantified using

(20) Non-additive genetic components contribute significantly to population-wide gene expression variationCell GenomicsJanuary 10, 2024Andreas Tsouris, Gauthier Brach, Joseph Schacherer, Jing Houprepared cDNA sequencing libraries using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, #E7765L) and following the manufacturer’s protocol. Briefly, 5 μL of purified mRNA is fragmented with a 15-min

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NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads

NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads from New England Biolabs

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