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NEBNext Ultra II Ligation Module
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NEBNext Ultra II Ligation Module
NEBNext Ultra II Ligation Module from New England Biolabs
Be the first to write a review!
Citations:
(14)
$403.00 Inquire
from
New England Biolabs for
NEBNext Ultra II Ligation Module
Description
NEBNext Ultra II Ligation Module - 24 rxns
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NEBNext Ultra II Ligation Module
Company
New England Biolabs
Price
$403.00 Inquire
Catalog Number
E7595S
Quantity
24 reactions
Applications
DNA Analysis, Ligation, NGS
Temperature
-20 C
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (14)
(1)
SCoTCH-seq reveals that 5-hydroxymethylcytosine encodes regulatory information across DNA strands
Proc Natl Acad Sci U S A
August 5, 2025
Jack S. Hardwick, Somdutta Dhir, Angie Kirchner, Angela Simeone, Sean M. Flynn, James M. Edgerton, Rafael de Cesaris Araujo Tavares, Isabel Esain-Garcia, David Tannahill, Paula Golder,
et al.
Repair/dA-Tailing Module, catalog number
E7546S
) and adapter-ligated (
NEB
Ultra II
Ligation
Module, catalog number
E7595S
) according to the manufacturer’s guidelines, except that 3.75 μL of a
1:1
mixture of custom, preannealed
(2)
Chromatin loops are an ancestral hallmark of the animal regulatory genome
Nature
May 7, 2025
Iana V. Kim, Cristina Navarrete, Xavier Grau-Bové, Marta Iglesias, Anamaria Elek, Grygoriy Zolotarov, Nikolai S. Bykov, Sean A. Montgomery, Ewa Ksiezopolska, Didac Cañas-Armenteros,
et al.
The Y-shaped Illumina adapters were ligated with
NEBNext Ultra II Ligation Module
(
NEB
, catalogue no.
E7595S
), and the final library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (
NEB
, catalogue
(3)
DNA methylation enables recurrent endogenization of giant viruses in an animal relative
Science Advances
July 12, 2024
Luke A. Sarre, Iana V. Kim, Vladimir Ovchinnikov, Marine Olivetta, Hiroshi Suga, Omaya Dudin, Arnau Sebé-Pedrós, Alex de Mendoza
using the NEBNext End repair/dA-tailing mix (NEB, E7546) and
NEBNext Ultra II Ligation Module
(
NEB
,
E7595S
). The final amplified libraries, comprising three biological replicates, were sequenced with NextSeq500
(4)
Hematopoietic stem cell gene editing rescues B-cell development in X-linked agammaglobulinemia
The Journal of allergy and clinical immunology
July 1, 2024
Bahal S, Zinicola M, Moula SE, Whittaker TE, Schejtman A, Naseem A, Blanco E, Vetharoy W, Hu YT, Rai R,
et al
.
New England Biolabs
). Linker
ligation
was performed with the
NEBNext Ultra II Ligation Module
(cat no:
E7595S
,
New England Biolabs
). Then 3 μL of USER Enzyme (cat no:
M5505S
,
New England Biolabs
) was added to each
(5)
Diet-induced changes in the jejunal microbiota of developing broilers reduce the abundance of Enterococcus hirae and Enterococcus faecium
BMC Genomics
June 23, 2024
Paul B. Stege, Dirkjan Schokker, Frank Harders, Soumya K. Kar, Norbert Stockhofe, Vera Perricone, Johanna M. J. Rebel, Ingrid C. de Jong, Alex Bossers
NEBNext® Ultra II FS DNA module (
E7810S
,
NEB
, Ipswich, USA) and the
NEBNext® Ultra II Ligation module
(
E7595S
,
NEB
, Ipswich, USA) according to manufacturer’s protocols. Libraries were sequenced on a NovaSeq 6000
(6)
Harnessing noncanonical crRNA for highly efficient genome editing
Nature Communications
May 7, 2024
Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhao
Y-adapter was ligated to the repaired genomic DNA by utilizing
NEBNext® Ultra™ II Ligation Module
(
NEB
,
E7595S
).
High-throughput sequencing
libraries were generated after tag-specific
amplification
, and the libraries
(7)
Assessing the effects of antiretroviral therapy-latency-reversing agent combination therapy on eradicating replication-competent HIV provirus in a Jurkat cell culture model
STAR Protocols
December 1, 2023
Kouki Matsuda, Benjy Jek Yang Tan, Samiul Alam Rajib, Kiyoto Tsuchiya, Yorifumi Satou, Kenji Maeda
"
New England Biolabs
(Ipswich, MA)",
E7546S
NEB
Next Ultra II
Ligation
Module,"
New England Biolabs
(Ipswich, MA)",
E7595S
AMPure XP Beads,"
Beckman Coulter
(Brea, CA)",
A63880
Q5 Hot Start High-Fidelity DNA Polymerase
,"
New England Biolabs
(8)
ERCC6L2 mitigates replication stress and promotes centromere stability
Cell reports
April 25, 2023
Carnie CJ, Armstrong L, Sebesta M, Ariza A, Wang X, Graham E, Zhu K, Ahel D.
RepairdA-Tailing Module,
New England Biolabs
,
E7546S
NEBNext Ultra II Ligation Module
,
New England Biolabs
,
E7595S
Phusion(R) High-Fidelity DNA Polymerase,
New England Biolabs
,
M0530S
QuikChange Lightning Site-Directed Mutagenesis Kit
(9)
A Rapid, Whole Genome Sequencing Assay for Detection and Characterization of Novel Coronavirus (SARS-CoV-2) Clinical Specimens Using Nanopore Sequencing
Frontiers in Microbiology
June 6, 2022
Maria T. Arévalo, Mark A. Karavis, Sarah E. Katoski, Jacquelyn V. Harris, Jessica M. Hill, Samir V. Deshpande, Pierce A. Roth, Alvin T. Liem, R. Cory Bernhards
Ligation
Sequencing
(Catalog #
E7180S
), barcoded using the
NEBNext Ultra II Ligation Module
(Catalog #
E7595S
,
NEB
) with ONT’s Native Barcoding Expansion 1–12 kit (EXP-NBD104). This barcoding process adds 42 min
(10)
Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in rats
Communications Biology
December 1, 2021
Robin Mesnage et al.
(11)
Microbiome connections with host metabolism and habitual diet from 1,098 deeply phenotyped individuals
Nature Medicine
February 1, 2021
Francesco Asnicar et al.
(12)
Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley Rats
Environmental Health Perspectives
January 27, 2021
Robin Mesnage et al.
(13)
A traditional evolutionary history of foot-and-mouth disease viruses in Southeast Asia challenged by analyses of non-structural protein coding sequences
Scientific Reports
December 1, 2018
Barbara Brito et al.
SISPA-generated libraries on an Illumina MiSeq, New England Biolabs (
NEB
), end prep and
ligation
modules (
E7546S
,
E7595S
) with Bio Scientific barcoded adapters (514113) were used. ORF consensus sequences were generated after
(14)
Genetic stability of foot-and-mouth disease virus during long-term infections in natural hosts
PLOS ONE
February 1, 2018
Lisbeth Ramirez-Carvajal, Steven J. Pauszek, Zaheer Ahmed, Umer Farooq, Khalid Naeem, Reed S. Shabman, Timothy B. Stockwell, Luis L. Rodriguez
For Illumina Miseq
sequencing
, the New England Biolabs (
NEB
) end prep and
ligation
modules (
E7546S
,
E7595S
) and Bio Scientific barcoded adapters (514113) were used. Libraries were purified, size-selected, visualized,
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NEBNext Ultra II Ligation Module from New England Biolabs
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