NEBNext Ultra II Ligation Module E7595S from New England Biolabs

Product Specs
ItemNEBNext Ultra II Ligation Module
CompanyNew England Biolabs
Price $403.00 Inquire
Catalog NumberE7595S
Quantity24 reactions
ApplicationsDNA Analysis, Ligation, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) SCoTCH-seq reveals that 5-hydroxymethylcytosine encodes regulatory information across DNA strandsProc Natl Acad Sci U S AAugust 5, 2025Jack S. Hardwick, Somdutta Dhir, Angie Kirchner, Angela Simeone, Sean M. Flynn, James M. Edgerton, Rafael de Cesaris Araujo Tavares, Isabel Esain-Garcia, David Tannahill, Paula Golder, et al.Repair/dA-Tailing Module, catalog number E7546S) and adapter-ligated (NEB Ultra II Ligation Module, catalog number E7595S) according to the manufacturer’s guidelines, except that 3.75 μL of a 1:1 mixture of custom, preannealed

(2) Chromatin loops are an ancestral hallmark of the animal regulatory genomeNatureMay 7, 2025Iana V. Kim, Cristina Navarrete, Xavier Grau-Bové, Marta Iglesias, Anamaria Elek, Grygoriy Zolotarov, Nikolai S. Bykov, Sean A. Montgomery, Ewa Ksiezopolska, Didac Cañas-Armenteros, et al.The Y-shaped Illumina adapters were ligated with NEBNext Ultra II Ligation Module (NEB, catalogue no. E7595S), and the final library was amplified using NEBNext High-Fidelity 2× PCR Master Mix (NEB, catalogue

(3) DNA methylation enables recurrent endogenization of giant viruses in an animal relativeScience AdvancesJuly 12, 2024Luke A. Sarre, Iana V. Kim, Vladimir Ovchinnikov, Marine Olivetta, Hiroshi Suga, Omaya Dudin, Arnau Sebé-Pedrós, Alex de Mendozausing the NEBNext End repair/dA-tailing mix (NEB, E7546) and NEBNext Ultra II Ligation Module (NEB, E7595S). The final amplified libraries, comprising three biological replicates, were sequenced with NextSeq500

(4) Hematopoietic stem cell gene editing rescues B-cell development in X-linked agammaglobulinemiaThe Journal of allergy and clinical immunologyJuly 1, 2024Bahal S, Zinicola M, Moula SE, Whittaker TE, Schejtman A, Naseem A, Blanco E, Vetharoy W, Hu YT, Rai R, et al.New England Biolabs). Linker ligation was performed with the NEBNext Ultra II Ligation Module (cat no: E7595S, New England Biolabs). Then 3 μL of USER Enzyme (cat no: M5505S, New England Biolabs) was added to each

(5) Diet-induced changes in the jejunal microbiota of developing broilers reduce the abundance of Enterococcus hirae and Enterococcus faeciumBMC GenomicsJune 23, 2024Paul B. Stege, Dirkjan Schokker, Frank Harders, Soumya K. Kar, Norbert Stockhofe, Vera Perricone, Johanna M. J. Rebel, Ingrid C. de Jong, Alex BossersNEBNext® Ultra II FS DNA module (E7810S, NEB, Ipswich, USA) and the NEBNext® Ultra II Ligation module (E7595S, NEB, Ipswich, USA) according to manufacturer’s protocols. Libraries were sequenced on a NovaSeq 6000

(6) Harnessing noncanonical crRNA for highly efficient genome editingNature CommunicationsMay 7, 2024Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin ZhaoY-adapter was ligated to the repaired genomic DNA by utilizing NEBNext® Ultra™ II Ligation Module (NEB, E7595S). High-throughput sequencing libraries were generated after tag-specific amplification, and the libraries

(7) Assessing the effects of antiretroviral therapy-latency-reversing agent combination therapy on eradicating replication-competent HIV provirus in a Jurkat cell culture modelSTAR ProtocolsDecember 1, 2023Kouki Matsuda, Benjy Jek Yang Tan, Samiul Alam Rajib, Kiyoto Tsuchiya, Yorifumi Satou, Kenji Maeda"New England Biolabs (Ipswich, MA)",E7546S NEB Next Ultra II Ligation Module,"New England Biolabs (Ipswich, MA)",E7595S AMPure XP Beads,"Beckman Coulter (Brea, CA)",A63880 Q5 Hot Start High-Fidelity DNA Polymerase,"New England Biolabs

(8) ERCC6L2 mitigates replication stress and promotes centromere stabilityCell reportsApril 25, 2023Carnie CJ, Armstrong L, Sebesta M, Ariza A, Wang X, Graham E, Zhu K, Ahel D.RepairdA-Tailing Module,New England Biolabs,E7546S NEBNext Ultra II Ligation Module,New England Biolabs,E7595S Phusion(R) High-Fidelity DNA Polymerase,New England Biolabs,M0530S QuikChange Lightning Site-Directed Mutagenesis Kit

(9) A Rapid, Whole Genome Sequencing Assay for Detection and Characterization of Novel Coronavirus (SARS-CoV-2) Clinical Specimens Using Nanopore SequencingFrontiers in MicrobiologyJune 6, 2022Maria T. Arévalo, Mark A. Karavis, Sarah E. Katoski, Jacquelyn V. Harris, Jessica M. Hill, Samir V. Deshpande, Pierce A. Roth, Alvin T. Liem, R. Cory BernhardsLigation Sequencing (Catalog # E7180S), barcoded using the NEBNext Ultra II Ligation Module (Catalog # E7595S, NEB) with ONT’s Native Barcoding Expansion 1–12 kit (EXP-NBD104). This barcoding process adds 42 min

(10) Multi-omics phenotyping of the gut-liver axis reveals metabolic perturbations from a low-dose pesticide mixture in ratsCommunications BiologyDecember 1, 2021Robin Mesnage et al.

(11) Microbiome connections with host metabolism and habitual diet from 1,098 deeply phenotyped individualsNature MedicineFebruary 1, 2021Francesco Asnicar et al.

(12) Use of Shotgun Metagenomics and Metabolomics to Evaluate the Impact of Glyphosate or Roundup MON 52276 on the Gut Microbiota and Serum Metabolome of Sprague-Dawley RatsEnvironmental Health PerspectivesJanuary 27, 2021Robin Mesnage et al.

(13) A traditional evolutionary history of foot-and-mouth disease viruses in Southeast Asia challenged by analyses of non-structural protein coding sequencesScientific ReportsDecember 1, 2018Barbara Brito et al.SISPA-generated libraries on an Illumina MiSeq, New England Biolabs (NEB), end prep and ligation modules (E7546S, E7595S) with Bio Scientific barcoded adapters (514113) were used. ORF consensus sequences were generated after

(14) Genetic stability of foot-and-mouth disease virus during long-term infections in natural hostsPLOS ONEFebruary 1, 2018Lisbeth Ramirez-Carvajal, Steven J. Pauszek, Zaheer Ahmed, Umer Farooq, Khalid Naeem, Reed S. Shabman, Timothy B. Stockwell, Luis L. RodriguezFor Illumina Miseq sequencing, the New England Biolabs (NEB) end prep and ligation modules (E7546S, E7595S) and Bio Scientific barcoded adapters (514113) were used. Libraries were purified, size-selected, visualized,

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NEBNext Ultra II Ligation Module

NEBNext Ultra II Ligation Module from New England Biolabs

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New England Biolabs

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NEBNext Ultra II Ligation Module from New England Biolabs

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