Luna Universal One-Step RT-qPCR Kit E3005L from New England Biolabs

Product Specs
ItemLuna Universal One-Step RT-qPCR Kit
CompanyNew England Biolabs
Price $591.00 Inquire
Catalog NumberE3005L
Quantity500 reactions
ApplicationsDNA Analysis, RT-qPCR
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Characterizing the Impact of Nucleoid-Associated Proteins on HU-DNA Interactions by Live-Cell Single-Molecule TrackingChemphyschemMay 14, 2026D. E. H. Fuller, X. Dai, L. A. McCarthy, L. E. Way, X. Wang, J. S. Biteenaveraging three independent measurements. For RT‐qPCR, a Luna Universal One‐Step RT‐qPCR kit (NEB #E3005L) was used. A master mix was prepared containing 1 × Luna Universal One‐Step Reaction Mix, 1 × Luna WarmStart

(2) Atlas of innate immune responses to experimental cholera and IL22 treatment demonstrates protection by mucus-secreting cellsbioRxivApril 1, 2026Masataka Suzuki, Yuko Hasegawa, Hailong Zhang, Zhu Liang, Guodong Tie, Ramesh A. Shivdasani, Matthew K. WaldorRNA was used for One-step cDNA synthesis and qPCR with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005L) according to the manufacture’s protocol. The amplification signal was analyzed using a StepOnePlus Real-Time PCR System

(3) Pooled CRISPRi screening reveals fungal-specific vulnerabilities across environments and genetic backgroundsbioRxivJanuary 10, 2026Lauren F Wensing, Philippe C Després, Desiree Francis, Meea Fogal, Anthony Hendriks, Nicholas C Gervais, Clara Fikry, Abdul-Rahman Adamu Bukari, Aleeza C Gerstein, Christina A Cuomo, et al.Kit (Luna Nanotech, cat. NK251–200). RT-qPCR was performed using a One-Step RT-qPCR kit (NEB, cat. E3005L). Real-time PCR was performed using a QuantStudio 3 Real-Time PCR system from Thermo Fisher Scientific

(4) Characterizing the Impact of Nucleoid-Associated Proteins on HU-DNA Interactions by Live-Cell Single-Molecule TrackingbioRxivDecember 29, 2025D. E. H. Fuller, X. Dai, L. A. McCarthy, L. E. Way, X. Wang, J. S. Biteenaveraging three independent measurements. For RT-qPCR, a Luna Universal One-Step RT-qPCR kit (NEB #E3005L) was used. A master mix was prepared containing 1× Luna Universal One-Step Reaction Mix, 1× Luna WarmStart

(5) Mammalian metaphase kinetochores are elastic and require condensin for robust structure and functionBIORXIVDecember 25, 2025Vanna M. Tran, Jinghui Tao, Caleb J. Rux, David Gomez Siu, Miquel Rosas-Salvans, Sophie Dumontsyringe and passed through 5–10 times. The Luna Universal One-Step RT-qPCR Kit (New England Biolabs; E3005L) was used with an input of 100 ng for each reaction. Both control and siSMC2 conditions were done with

(6) Rif-seq reveals Caulobacter crescentus mRNA decay is globally coordinated with transcription and translationCell ReportsDecember 23, 2025Kulathungage H. Dilrangi, James R. Aretakis, Kavya Vaidya, Nadra Al-Husini, Nisansala S. Muthunayake, Sangjin Kim, Jared M. Schraderlaboratories,inc",Cat#2701 Nuclease free distilled water,Invitrogen,Cat#10977049 Luna® Universal One-Step RT-qPCR Kit,NEB,Cat#E3005L DEPC water,Sigma-Aldrich,Cat#693520-1L "Tris (1 M), pH 8.0, RNase-free",Invitrogen,Cat#AM9855G MgCl2 (1 M

(7) cAMP promotes acute lysosome biogenesis through TFEB nuclear import-export dynamicsbioRxivNovember 28, 2025Saumya Bhatt, Mohammad Ali Abbas Abbas Zaidi, Nicholas Woods, Cody N. Rozeveld, Micah B. Schotttemplate in a 20 μL reaction using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs, Cat. No. E3005L), following the manufacturer’s instructions. Reactions were performed on a Bio-Rad CFX96 Touch™ Real-Time PCR Detection System

(8) Systematic genome-wide mapping of host determinants of bacteriophage infectivityCell SystemsNovember 19, 2025Chutikarn Chitboonthavisuk, Cody Martin, Phil Huss, Jason M. Peters, Karthik Anantharaman, Srivatsan Ramanverified with A260/A280~2.00. RT-qPCR was conducted using Luna®Universal One-Step RT-qPCR Kit (NEB E3005L) following the manufacturer’s documentation. Primer efficiency was validated using RNA samples from

(9) Hepatitis B virus is a stealth virus that minimizes proteomic and secretomic changes in primary human hepatocytesJournal of General VirologyNovember 7, 2025Karolína Štaflová, Kamila Clarová, Michal Doležal, Martin Hubálek, Alena Křenková, Jan Hodek, Iva Pichová, Aleš Zábranskýtranscription quantitative PCR (RT-qPCR) was performed using Luna Universal One-Step RT-qPCR Kit (#E3005L, New England Biolabs) on a CFX Opus 96 Real-Time PCR System (Bio-Rad). The GAPDH gene was used as a

(10) Protocol for isolating and characterizing effector functions of murine bone marrow-derived eosinophils following bacterial challengeSTAR ProtocOctober 10, 2025Katelyn M. Parrish, Tyler L. Williams, Monica C. Gestalassays ,, PureLink RNA extraction kit,Invitrogen,Cat. #2365053 Luna one-step qRT-PCR kit,New England Biolabs,Cat. #E3005L Thelper v.13 LEGENDplex chemokine ELISA,BioLegend,Cat. #740451 Thelper v.13 LEGENDplex cytokine ELISA,BioLegend,Cat.

(11) Atlas of amnion development during the first trimester of human pregnancyNat Cell BiolJuly 14, 2025Wenqi Hu, Carmen Sancho-Serra, Carlos W. Gantner, Hanna M. Szafranska, Nita Solanky, Kate Metcalfe, Roser Vento-Tormo, Magdalena Zernicka-Goetzng of RNA per reaction was used with the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, E3005L). Reactions were carried out under the following cycling conditions: reverse transcription at 55 °C

(12) Activation of bacterial programmed cell death by phage inhibitors of host immunityMolecular CellMay 1, 2025Sukrit Silas, Héloïse Carion, Kira S. Makarova, Eric S. Laderman, Thomas Todeschini, Pradeep Kumar, Matthew Johnson, Michael Bocek, Franklin L. Nobrega, Eugene V. Koonin, et al.NEBuilder HiFi DNA assembly master mix,NEB,E2621L 1x dsDNA HS assay kit,Thermo Fisher Scientific,Q33231 Luna® Universal One-Step RT-qPCR Kit,NEB,E3005L AMpure XP beads,Beckman Coulter,A63881 ,, Deposited data,Deposited data,Deposited data Sequencing

(13) Jumbo phage killer immune system targets early infection of nucleus-forming phagesCellApril 17, 2025Li Yuping, Linlin Guan, Isabelle Becher, Kira S. Makarova, Xueli Cao, Surabhi Hareendranath, Jingwen Guan, Frank Stein, Siqi Yang, Arne Boergel, et al.Kit,Qiagen,Cat# 10196-4 Turbo DNA-free kit,Invitrogen,Cat# AM1907 Luna® Universal One-Step RT-qPCR Kit,NEB,Cat# E3005L PerfeCTa® SYBR® Green SuperMix,QuintaraBio,Cat# 95054-500 SMARTer Stranded RNA-Seq kit,Takara,Cat#

(14) Cellular Receptor Tyrosine Kinase Signaling Plays Important Roles in SARS-CoV-2 InfectionPathogensMarch 31, 2025Shania Sanchez, Brigitte H. Flannery, Hannah Murphy, Qinfeng Huang, Hinh Ly, Yuying Liangreverse-transcribed PCR (RT-qPCR) using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs, E3005L, Ipswich, MA, USA) was performed on the extracted RNA samples in duplicates using primers specific for

(15) Mitochondrial injury and complement dysregulation are drivers of pathological inflammation in viral myocarditisJournal of VirologyFebruary 25, 2025Yasir Mohamud, Amirhossein Bahreyni, Sinwoo Wendy Hwang, Jingfei Carly Lin, Zhihan Claire Wang, Jingchun Zhang, Honglin Luoreaction containing 1 µg of RNA using the Luna Universal One-Step RT-qPCR kit (New England Biolabs, #E3005L) and normalized to ACTB mRNA according to the manufacturer’s instructions. The PCR reaction was performed

(16) Comprehensive dissection of cis-regulatory elements in a 2.8 Mb topologically associated domain in six human cancersNat CommunFebruary 13, 2025Christina M. Caragine, Victoria T. Le, Meer Mustafa, Bianca Jay Diaz, John A. Morris, Simon Müller, Alejandro Mendez-Mancilla, Evan Geller, Noa Liscovitch-Brauer, Neville E. SanjanaFAM/ZEN/Iowa Black FQ for the B2M normalizer (IDT), and Luna Universal One-Step RT qPCR Master Mix kit (NEB E3005L) and Tween-20 (Sigma-Aldrich P1379). All dPCR primer and probe sequences are in Supplementary Data 7.

(17) Molecular Mechanisms of Acanthamoeba castellanii Response to Different Sources of Oxidative StressJournal of Proteome ResearchFebruary 7, 2025Kateřina Ženíšková, Pavel Stopka, Tania Martín-Pérez, Guillaume Chevreux, Maria Grechnikova, Eliška Drncová, Ronald Malych, Jan Mach, Julia Walochnik, Jean-Michel Camadro, et al.was performed in a CFX96 thermocycler (Bio-Rad, USA) using the Luna Universal One-Step RT-qPCR kit (E3005L, New England BioLabs, USA). The reaction mixture (20 μL per reaction) contained 10 μL of Luna Universal

(18) Alphaviral genetic background of self-amplifying RNA enhances protein expression and immunogenicity against SARS-CoV-2 antigenMolecular TherapyFebruary 5, 2025Irafasha C. Casmil, Nuthan V. Bathula, Cynthia Huang, Christopher J. Wayne, Evan S. Cairns, Josh J. Friesen, Shekinah K. Soriano, Suiyang Liao, Chia H. Ho, Kristen Y.S. Kong, et al.Monarch Total RNA miniprep kit (NEB #T2010) including the DNase treatment step. The Luna one-step RT-qPCR kit (NEB #E3005L) was used according to manufacturer’s instructions. We used 100 ng of total RNA as template. Primer

(19) Pharmacological Modulation of the Unfolded Protein Response as a Therapeutic Approach in Cutaneous T-Cell LymphomaBiomoleculesJanuary 7, 2025St Thomas N, Christopher BN, Reyes L, Robinson RM, Golick L, Zhu X, Chapman E, Dolloff NG.transcribed using the Luna Universal One-Step RT-qPCR Kit (New England BioLabs; Ipswich, MA, USA Catalog No. E3005L). Samples were run using the QuantStudio 3 Real-Time PCR System (AppliedBiosystems, ThermoFisher) and

(20) Regulation of human interferon signaling by transposon exonizationCellDecember 26, 2024Giulia Irene Maria Pasquesi, Holly Allen, Atma Ivancevic, Arturo Barbachano-Guerrero, Olivia Joyner, Kejun Guo, David M. Simpson, Keala Gapin, Isabella Horton, Lily L. Nguyen, et al.expression levels were quantified using the Luna Universal One-Step RT-qPCR Kit (New England Biolabs #E3005L) according to the manufacturer’s instructions. In brief, for each reaction 25ng of RNA was combined

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Luna Universal One-Step RT-qPCR Kit

Luna Universal One-Step RT-qPCR Kit from New England Biolabs

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New England Biolabs

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Luna Universal One-Step RT-qPCR Kit from New England Biolabs

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