Monarch Genomic DNA Purification Kit T3010S from New England Biolabs

Product Specs
ItemMonarch Genomic DNA Purification Kit
CompanyNew England Biolabs
Price $166.00 Inquire
Catalog NumberT3010S
Quantity50 preps
ApplicationsDNA Manipulation
Temperature25 C
Sample TypeBlood samples, saliva, swabs, tissue samples, cultured cells

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multimodal profiling reveals cell type-specific pseudouridine modification and density-dependent translational regulationNucleic Acids ResApril 21, 2026Caroline A McCormick, Michele Meseonznik, Yuchen Qiu, Oleksandra Fanari, Priyanka Goyal, Mitchell Thomas, Mina Shokoufandeh, Yifang Liu, Dylan Bloch, Cole Greenfield, et al.genomic DNA (gDNA). gDNA extraction was performed using a Monarch Genomic DNA Purification Kit (NEB, T3010S) following the manufacturer’s protocol. Polymerase chain reaction (PCR) primers were designed to amplify

(2) Incorporation of genome-bound cellular proteins into HIV-1 particles regulates viral infectionCell RepApril 7, 2026Manuel Garcia-Moreno, Azman Embarc-Buh, Robin Truman, Marko Noerenberg, Louisa Iselin, Honglin Chen, Caroline E. Lenz, Jeffrey Y. Lee, Kate Dicker, Snehith Dyavari Shetty, et al.editing efficiency by genomic DNA extraction (Monarch Genomic DNA Purification Kit, New England Biolabs, #T3010S) followed by PCR, PCR cleanup (QIAquick PCR Purification Kit, Qiagen, #28104), sequencing and sequence

(3) Efficient multi-kilobase knock-ins in mice and cell lines using CRISPR/Cas9 and rAAV donors with unbiased whole-genome characterization by LOCK-seqNucleic Acids ResApril 7, 2026Monica F Sentmanat, Zi Teng Wang, Evguenia Kouranova, Samuel T Peters, Wan Ching Chan, Jed Lin, Yong Miao, J Michael White, Mia Wallace, Xiaoxia Cui-targeted sequencing Genomic DNA from tail clips was isolated with the Monarch Genomic DNA Purification Kit (NEB, cat. #T3010S). nCATS was performed using the Oxford Nanopore’s Cas9 Sequencing Kit (cat. #SQK-CS9109) according to

(4) A self-complementary recombinant adeno-associated virus vector coding for an anchorless prion protein carrying the G127V mutation extends survival in a rodent prion disease modelbioRxivMarch 30, 2026Thomas Zerbes, Claire Verkuyl, Cunjie Zhang, Sophie Grunnesjoe, Shehab Eid, Hamza Arshad, Wenda Zhao, Zahra Nasser, Teaghan O’Shea, Ari Belotserkovsky, et al.mice were extracted from brain homogenates with the Monarch Spin gDNA Extraction Kit (catalog number T3010S, New England Biolabs). For the subsequent PCR reaction, 50 ng of gDNA was amplified with Q5 Hot Start High-Fidelity 2X Master Mix

(5) MASTR-seq enables multiplexed analysis of short tandem repeats with sequencingCell Rep MethodsMarch 23, 2026Chuanbin Su, Han-Seul Ryu, Keerthivasan Raanin Chandradoss, Thomas Malachowski, Ravi Boya, Linda Zhou, Hoa Emma Nguyen, Esteban O. Mazzoni, Kristen J. Brennand, Jennifer E. Phillips-CreminsNanopore Technologies,Cat# SQK-NBD114.24 Monarch Spin gDNA Extraction Kit,New England Biolabs,Cat# T3010S Flow Cell Priming Kit,Oxford Nanopore Technologies,Cat# EXP-FLP004 Qubit™ Broad Range dsDNA Quantitation,Invitrogen,Cat#

(6) NELF prevents transcriptional readthrough into DNA replication zones in cancer cellsEMBO ReportsFebruary 20, 2026Chihiro Nakayama, Qi Fang, Yasukazu Daigaku, Yuki Aoi, Shoko Ito, Mami Takahashi, Reo Shimatani, Tamiko Minamisawa, Yagiz Ozturk, Hiroshi Kimura, et al.Midi Kit with 100/G Genomic-tips,QIAGEN,Cat# 13343 Monarch® Genomic DNA Purification Kit,NEB,Cat# T3010S DNA Clean & Concentrator Kit,Zymo Research,Cat# D4013 Monarch® Genomic DNA Purification Kit,NEB,Cat#

(7) The Hsp40 co-chaperone DNAJC7 regulates polyglutamine aggregation and exhibits context-dependent effects on polyglycine aggregationJournal of Biological ChemistryFebruary 16, 2026Biswarathan Ramani, Kean Ehsani, Martin Kampmannmice, DNA was purified from ear punches following the manufacturer’s instructions (New England Biolabs, T3010S). The eluted DNA (1 μl) was used for PCR amplification using primers and cycling conditions detailed

(8) Identification of transgene insertion sites via short- and long-read whole genome sequencingbioRxivFebruary 5, 2026L Kaplan, S J Edgerton, B D Mahoney, C A Ray, T A RehRlbp1-CreER:LNL-tTA:tetO-mAscl1-IRES-GFP. Genomic DNA was extracted and purified with the Monarch Spin gDNA extraction kit (NEB #T3010S, New England Biosciences). The quality and quantity of extracted DNA was evaluated using a Nanodrop 2000 spectrophotometer

(9) Targeted editing of pericentromeric satellite DNA alters sensitivity to meiotic drivebioRxivJanuary 30, 2026D.G. Eickbush, J. Rahmat, M. Lindsay, J. Bowers, N.J. Fuda, A.M. Larracuentegenomic DNA from 20 mg of 5–7 day old female flies using the NEB Monarch spin gDNA extraction kit (T3010S) following a modified protocol45. We sequenced the gDNA using the custom eukaryotic genome sequencing

(10) Assessing the Toxoplasma Tachyzoite Cell Cycle Phases Using Fluorescent Ubiquitination-Based Cell Cycle IndicatorBIO-PROTOCOLJanuary 20, 2026Mrinalini Batra, Elena S. Suvorovatechnologies, IDT, 25 nmole) 14. DNA Extraction kit (Monarch Genomic DNA purification Kit, NEB, catalog number: T3010S) 15. Miniprep kit (Qiagen, catalog number: 27106) 16. PCR Cleanup kit (Macherey Nagel, catalog number:

(11) Optimized CRISPR-Cas9 system for efficient engineering of ecDNA in cancer cellsNucleic Acids ResearchJanuary 14, 2026Yohei Sugimoto, Takeru Kachi, Yu Watanabe, Mei Kubokawa, Koichi Ogami, Masaki Kawamata, Seiko Yoshino, Hiroshi I Suzukidescribed earlier. Genomic DNA (gDNA) was then extracted with the Monarch Spin gDNA Extraction Kit (NEB, T3010S). T7E1 assays were performed as previously described [26]. Briefly, Cas9 target sites were amplified

(12) Complete genome sequence of Thioclava strain BDS19, isolated from coastal marsh sedimentMicrobiology Resource AnnouncementsJanuary 13, 2026Biagio DiSalvo, Blair G. Paul, Zoe G. Cardonovernight. Genomic DNA was extracted using the NEB Monarch Genomic DNA Extraction and Purification Kit (#T3010S, NEB, USA). Libraries were generated by Plasmidsaurus (Louisville, KY, USA) using an Ultra-long Sequencing

(13) Salivary microbiome and periodontopathogen/denitrifying bacteria associated with gingivitis and periodontitis in people with type 2-diabetesF1000ResJanuary 13, 2026Endang Bachtiar, Boy M. Bachtiar, Dicky L Tahapary, Turmidzi Fath, Citra Fragrantia Theodora, Natalina Haerani, Selvi Nafisa Shahab, Yuniarti Soeroso, Ardy Wildan, Fergie Marie Joe Grizella Runtu, et al.individually and were not pooled. DNA was extracted using the Monarch ® ,™ Genomic DNA purification kit, NEB #T3010S/L (New England Biolabs, Bruningstrasse Frankfurt am Main, Germany) and quantified using a Qubit 2.0 Fluorometer

(14) Investigating epigenetic biomarkers of age, sex, and disease in captive South African cheetahs (Acinonyx jubatus jubatus)PLOS OneJanuary 13, 2026Michelle Cristi Ysrael, Steven Kubiski, Caroline E. MooreDNA was extracted using Monarch Genomic DNA Purification Kit (New England Biolabs, Ipswich, MA; Cat.T3010S) or DNeasy Blood and Tissue Kit (Qiagen, Valencia, CA; Cat. No./ ID: 69504) with the following modifications

(15) Basal IFNλ2/3 signaling is required for ISG expression and viral control in human intestinal epithelial cellsPLOS PathogensJanuary 12, 2026Yagmur Keser, Zehra Sena Bumin, Amelia Perez Valiente, Sorin O. Jacobs, Steeve Boulant, Megan L. Staniferharvested, and genomic DNA (gDNA) was isolated by using Monarch Genomic DNA Purification Kits (NEB #T3010S) according to manufacturers’ protocol. To amplify the genomic loci of IFNλ1, IFNλ2, and IFNλ3, PCR amplification

(16) Aging-induced hepatocyte CD44 drives IL6/STAT3 signaling and associates with impaired neighboring T cell functionbioRxivDecember 26, 2025Armin Gandhi, Kathryn Lande, Yichen Li, Filipe A. Hoffman, Michael LaPorte, Shirong Tan, Garrett Evensen, Benji Portillo, Marcos Garcia Teneche, Rouven Arnold, et al.about 50 mg frozen liver tissue using Monarch Genomic DNA Purification kit (New England Biolabs, Ref T3010S) and PCR was performed using 2X Taq PCR Master Mix (APExBIO, Ref K1034), 100 ng of gDNA and 1 μL of

(17) Salivary microbiome and periodontopathogen/denitrifying bacteria associated with gingivitis and periodontitis in people with type 2-diabetesF1000ResDecember 9, 2025Endang Bachtiar, Boy M. Bachtiar, Dicky L Tahapary, Turmidzi Fath, Citra Fragrantia Theodora, Natalina Haerani, Selvi Nafisa Shahab, Yuniarti Soeroso, Ardy Wildan, Fergie Marie Joe Grizella Runtu, et al.individually and were not pooled. DNA was extracted using the Monarch ® ,™ Genomic DNA purification kit, NEB #T3010S/L (New England Biolabs, Bruningstrasse Frankfurt am Main, Germany) and quantified using a Qubit 2.0 Fluorometer

(18) Mapping single-cell diploid chromatin fiber architectures using DAF-seqNature BiotechnologyDecember 3, 2025Elliott G. Swanson, Yizi Mao, Benjamin J. Mallory, Mitchell R. Vollger, Stephanie C. Bohaczuk, Christopher B. Oliveira, Daniel B. Lyon, Jane Ranchalis, Nancy L. Parmalee, Barak A. Cohen, et al.5%. Genomic DNA was extracted using the Monarch Genomic DNA Purification Kit (New England Biolabs, T3010S) and quantified using a Qubit 1X dsDNA High-Sensitivity kit (Invitrogen, Q33231). PCR Amplification

(19) Bacteria enable tolerance to bile salt exposure in an immune-competent human intestinal modelGut microbesDecember 1, 2025Li Y, Wang CM, Zhao P, Fernez MT, Carrier RL.DNA was extracted from the EMA-treated sample by Monarch gDNA Purification Kit (New England Biolab, T3010S) according to manufacturer’s instructions. The density of each strain and consortium composition were

(20) The CIP2A-TOPBP1 axis facilitates mitotic DNA repair via MiDAS and MMEJNature CommunicationsNovember 27, 2025Peter R. Martin, Jadwiga Nieminuszczy, Zuza Kozik, Nihal Jakub, Szymon Kowalski, Maxime Lecot, Julia Vorhauser, Karen A. Lane, Alexandra Kanellou, Jörg Mansfeld, et al.days before harvesting 500,000 cells for gDNA extraction using the Monarch gDNA extraction kit (NEB, T3010S) while maintaining the remaining cells in continuous culture. The gDNA was subjected to Phusion polymerase

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(1)

8/27/2020Genomic DNA Purification Kit Works Well! Atul SinghI will highly recommend for genomic DNA isolation for WGS.

Monarch Genomic DNA Purification Kit

Monarch Genomic DNA Purification Kit from New England Biolabs

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New England Biolabs

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Monarch Genomic DNA Purification Kit from New England Biolabs

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