NEBNextUltraII DNA Library Prep with Sample Purification Beads E7103S from New England Biolabs

Product Specs
ItemNEBNextUltraII DNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $658.00 Inquire
Catalog NumberE7103S
Quantity24 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencingSTAR ProtocApril 21, 2026Xuan Jiang, Wenxiang ZhangAlternatives: This protocol uses NEBNext Ultra II DNA Library Prep Kit with Sample Purification Beads (NEB #E7103S) to construct library. If NEBNext Ultra II DNA Library Prep Kit is unavailable, optimized library building

(2) TRBC1/TRBC2 RNA In Situ Hybridization as a Diagnostic Approach for Canine and Feline T-Cell Lymphoma: A Proof-of-Concept StudyVeterinary SciencesMarch 28, 2026Honoria M. E. Brown, Jonathan J. Wilson, Daniel Rodgers, Shelley C. Evans, Julia Jones, Jianxiong Pang, Joy Archer, Fernando Constantino-Casas, Sam Parsons, Adam G. Scott, et al.combination with NEBNext® Multiplex Oligos for Illumina® (Unique Dual Index UMI Adaptors DNA) (NEB #E7103S/L), following the manufacturer’s instructions. AMPure beads (Product No: A63880; Beckman Coulter) and

(3) The Rpd3 histone deacetylase is a critical regulator of temperature-mediated morphogenesis and virulence in the human fungal pathogen HistoplasmaPLOS BiologyMarch 17, 2026Nebat Ali, Mark Voorhies, Rosa A. Rodriguez, Anita SilDNA were used for Illumina library preparation using the NEBNext Ultra II DNA Library Prep Kit (NEB E7103S) and Illumina dual-indexed primer pairs sourced from the UCSF CAT. The quality and size distribution

(4) Cell-SELEX identifies a DNA aptamer for highly selective in vivo siRNA delivery in cholangiocarcinomaJHEP ReportsMarch 16, 2026Brandon A. Wilbanks, Ayano Kabashima, Enis H. Ozmert, Jack W. Sample, Julia Driscoll, Danielle M. Carlson, Caroline D. Doherty, Brooke Kimball, Jayla Millender, Irene K. Yan, et al.DNA was used as input into the NEBNext Ultra II DNA Library Prep with Sample Purification Beads (NEB, E7103S). Volumes recommended by the manufacturer were halved, and, in the purification of adaptor-ligated DNA

(5) Large-scale distribution of cestode infections in wild gentoo penguins and their impact on the host microbiomeSci RepMarch 9, 2026Chloe Kaczvinsky, Hila Levy, Stephen Preston, Casey Youngflesh, Gemma Clucas, Heather J. Lynch, Maria D. Christodoulou, Tom Hart, Adrian L. Smiththen prepared with the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs, UK, E7103S) according to the manufacturer’s instructions, with no size selection. NEBNext Multiplex Oligos for

(6) Heritable changes in chromatin contacts associated with transgenerational susceptibility to diet-induced insulin dysregulation and obesityNature CommunicationsFebruary 12, 2026Richard C. Chang, Riann J. Egusquiza, Angélica Amorim Amato, Zhuorui Li, Alivia L. Dougherty, Kaitlin T. To, Michelle Avila, Erika M. Joloya, Hailey B. Wheeler, Angela Nguyen, et al.Motif, Carlsbad, CA USA) using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, Cat# E7103S) and sequenced with Illumina NovaSeq X Plus to generate 150 + 150 paired-end reads. FASTQ reads were

(7) Immunoglobulin germline reference sequences enable investigations and reveal insights into bat-specific immunityiScienceDecember 19, 2025Ashley B. Reers, Shijun Zhan, Taylor Pursell, Clara Reasoner, Natasha Hodges, Tanya M. Lama, Tony Schountz, Hannah K. Frankprepared from cleaned PCR reactions using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7103S) and NEBNext Multiplex Oligos for Illumina (NEB #E7600S) according to the manufacturer’s instructions.

(8) Non-coding genetic variants underlying higher prostate cancer risk in men of African ancestryNature CommunicationsNovember 20, 2025Shan Li, Kaniz Fatema, Nidharshan Sundarraj, Arashdeep Singh, Padma Sheila Rajagopal, Dimple Notani, David Y. Takeda, Sridhar HannenhalliSequencing libraries were prepared by NEBNext UltraII DNA Library Prep Kit (New England Biolabs cat#E7103S) per manufacturer’s instructions and sequenced on the Illumina NextSeq2000 platform at the CCR Genomics

(9) Lysine-specific histone demethylase complex restricts Epstein–Barr virus lytic reactivationNature MicrobiologyOctober 31, 2025Yifei Liao, Jinjie Yan, Isabella Y. Kong, Zhixuan Li, Weiyue Ding, Sarah Clark, Davide Maestri, Tetsuya Yoshida, Lisa Giulino-Roth, Benjamin E. Gewurzsubjected to library preparation using NEBNext Ultra II DNA Library Prep Kit (New England Biolabs, #E7103S) and were sequenced on an Illumina NovaSeq 6000 using PE150 Sequencing Strategy by Novogene Corporation.

(10) Disease-linked regulatory DNA variants and homeostatic transcription factors in epidermisNature communicationsSeptember 25, 2025Porter DF, Meyers RM, Miao W, Reynolds DL, Hong AW, Yang X, Srinivasan S, Mondal S, Siprashvili Z, Fabo T, et al.D4014), eluting in 27 μL water. Library was prepared using the NEB Ultra II DNA library prep kit (NEB, E7103S) following dx.doi.org/10.17504/protocols.io.bagaibse116, with the following modifications: the adapter

(11) RNA-binding proteins mediate the maturation of chromatin topology during differentiationNature Cell BiologySeptember 8, 2025Bondita Dehingia, Małgorzata Milewska-Puchała, Marcin Janowski, Mahmoud-Reza Rafiee, Misbah Abbas, Aleksandra Piotrowska, Jan Senge, Piotr Blaut, Dietrich Walsh, Jacqueline Severino, et al.modification at the library preparation step, which was done using the NEBNext Ultra II DNA Library Kit (NEB, E7103S), according to the manufacturer’s instructions. Libraries were sequenced (2 × 150 paired end) using

(12) Multiple myeloma associated long non-coding RNA PLUM confers chemoresistance by enhancing PRC2 mediated UPR pathway activationNature CommunicationsSeptember 1, 2025Kamalakshi Deka, Jean-Michel Carter, Akash Bahai, Daniel Aron Ang, Nicholas Sim, Hooi Yan Chong, Guan Hwee Bernard Lee, Suet Mien Tan, Wee Joo Chng, Dennis Kappei, et al.Subsequently, library was prepared using NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (E7645S; NEB and E7103S; NEB) according to manufacturer’s recommendations. After library preparation, the samples were quantitated

(13) The embryonic lethal mutation zyg-10(b261) is an allele of the atx-2 gene and disrupts multiple aspects of early embryogenesisMicroPubl BiolAugust 28, 2025Zachary G. Bell, Harold E. Smith, Kevin F. O'Connellprepared from the sheared DNA using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina®(Catalog No. E7103S, New England Biolabs, Ipswich, USA). The concentration of each library was again measured on the Mini-Fluorometer

(14) Accumulation of Epigenetic Noise in the Aging Corneal Epithelium and Its Possible MechanismFASEB JJune 15, 2025Galina Dvoriantchikova, Michelle Fleishaker, Dmitry Ivanov(IP) and Input (IN) DNA were used to prepare libraries using the NEBNext Ultra II DNA Library Prep (E7103S, NEB, Ipswich, MA, USA). The hMeDIP‐seq libraries were quantified using Qubit 4, and sizes were evaluated

(15) RNF20 links the DNA damage response and metabolic rewiring in lung cancer through HIF1αNat CommunMay 28, 2025Hao Liu, Yongqin Tang, Anshu Singh, Joaquim Vong, Julio Cordero, Arthur Mathes, Rui Gao, Yanhan Jia, Boyan K. Garvalov, Till Acker, et al.28004). Libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645S/L, E7103S/L) according to the manufacturer’s instructions. ChIP-Seq reads were trimmed using Trimmomatic, at a

(16) Tripartite phosphorylation of SPT5 by CDK9 times pause release and tunes elongation rate of RNA polymerase IIMolecular CellMay 1, 2025Rui Sun, Robert P. Fishercommercial assays,Critical commercial assays NEBNext® Ultra™ II DNA Library Prep,New England Biolabs,Cat. # E7103S NEBNext® Ultra™ II Directional RNA Library Prep,New England Biolabs,Cat. # E7760S NEBNext® Poly(A)

(17) The expansion and diversification of epigenetic regulatory networks underpins major transitions in the evolution of land plantsMolecular Biology and EvolutionApril 1, 2025Romy Petroll, Ranjith K Papareddy, Rafal Krela, Alice Laigle, Quentin Riviere, Kateřina Bišová, Iva Mozgová, Michael BorgSequencing libraries were made with 5 ng DNA using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB #E7103S/L) following the manufacturer's instructions. Paired-end 150 bp Illumina Sequencing was performed using

(18) Radial glia integrin avb8 regulates cell autonomous microglial TGFβ1 signaling that is necessary for microglial identityNat CommunMarch 22, 2025Gabriel L. McKinsey, Nicolas Santander, Xiaoming Zhang, Kilian L. Kleemann, Lauren Tran, Aditya Katewa, Kaylynn Conant, Matthew Barraza, Kian Waddell, Carlos O. Lizama, et al.for Histones (Diagenode, C01010059) and NEBNext® UltraTM II DNA Library Prep Kit for Illumina® (NEB, E7103S). Briefly, microglia were sorted from Itgb8fl/fl;Emx1Cre mouse brain cortex and were pooled from 3-4

(19) Chromosomal domain formation by archaeal SMC, a roadblock protein, and DNA structureNat CommunFebruary 19, 2025Kodai Yamaura, Naomichi Takemata, Masashi Kariya, Ayami Osaka, Sonoko Ishino, Masataka Yamauchi, Tomonori Tamura, Itaru Hamachi, Shoji Takada, Yoshizumi Ishino, et al.library construction with NEBNext Ultra II DNA Library Prep with Sample Purification Beads (New England Biolabs, E7103S). Reaction was performed according to the manufacturer’s instructions with size selection for a 300–400

(20) PRDM9 drives the location and rapid evolution of recombination hotspots in salmonid fishPLoS BiolJanuary 6, 2025Marie Raynaud, Paola Sanna, Julien Joseph, Julie Clément, Yukiko Imai, Jean-Jacques Lareyre, Audrey Laurent, Nicolas Galtier, Frédéric Baudat, Laurent Duret, et al.H3K36me3 ChIP-seq, we generated libraries using the NEBNext Ultra II protocol for Illumina (NEB, E7645S-E7103S), with minor adjustments. For DMC1-SSDS, we generated libraries following the Illumina TruSeq protocol

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NEBNextUltraII DNA Library Prep with Sample Purification Beads

NEBNextUltraII DNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII DNA Library Prep with Sample Purification Beads from New England Biolabs

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