NEBNextUltraII RNA Library Prep with Sample Purification Beads E7775S from New England Biolabs

Product Specs
ItemNEBNextUltraII RNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $1,005.00 Inquire
Catalog NumberE7775S
Quantity24 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Memory T cell rapid recall is driven by memory-specific AP-1 recruitment determined by epigenome and co-factor interactionsbioRxivFebruary 19, 2026Adenike R. Shittu, Andrew VonHandorf, Michael Kotliar, Valerii Pavlov, Sarah Potter, Xiaoting Chen, Mathew T. Weirauch, Artem BarskiEnzyme,Diagenode,"Denville, NJ, USA" T2010S,Monarch Total RNA Miniprep Kit,New England Biolabs,"Ipswich, MA, USA" E7775S,NEBNext Ultra II RNA Library Prep,New England Biolabs,"Ipswich, MA, USA" T2010S,NEB Total RNA Miniprep

(2) Upregulation of TNFR2 Precedes TOX Expression by Exhausted T cells and Restricts Antitumor and Antiviral ImmunityClinical Cancer ResearchFebruary 17, 2026Alexandra M. Hoyt-Miggelbrink, Jessica Waibl Polania, Luke Wachsmuth, Selena Lorrey, Aditya Mohan, Andrew Hardigan, Emily Blandford, Emily Lerner, Daniel Wilkinson, Kelly M. Hotchkiss, et al.Samples were processed using the NEBNext Ultra II RNA Library Prep Kit from New England Biolabs (cat. #E7775S) and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer pairs; NEB, cat. #E6440S)

(3) Turbo-RIP: A Protocol for TurboID-based RNA Immunopurification to Map RNA Landscapes in Plant Biomolecular CondensatesBIO-PROTOCOLFebruary 5, 2026Zhi Zhang, Yanting Xu, Hanxiang Liu, Chen Liu, Panagiotis Nikolaou MoschouNEBNext® UltraTM II RNA Library Prep with sample purification beads (New England BioLab, catalog number: E7775S) 74. NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioNordika BioLab,

(4) ALKBH5-Mediated ITGB1 m6A Modification in Ovarian Cancer Progression and Immune EvasionNPJ Precis OncolDecember 9, 2025Zheng He, Dalu Wang, Weijia Zhao, Mingyue Ma, Cheng Duutilized to deplete ribosomal RNA (rRNA) from the total RNA. The NEB Next Ultra RNA Library Prep Kit (#E7775S, NEB, USA) was employed for library construction for sequencing on the Illumina platform. Subsequently,

(5) Shoot Nitrate Status Regulates Arabidopsis Shoot Growth and Systemic Transcriptional Responses via Shoot Adenosine Phosphate‐Isopentenyltransferase 3Physiologia PlantarumSeptember 25, 2025Kota Monden, Takamasa Suzuki, Mikiko Kojima, Yumiko Takebayashi, Daisuke Sugiura, Tsuyoshi Nakagawa, Hitoshi Sakakibara, Takushi Hachiyalibraries were constructed using a NEBNext Ultra II RNA Library Prep with Sample Purification Beads (E7775S, New England Biolabs), a NEBNext Poly(A) mRNA Magnetic Isolation Module (E7490S, New England Biolabs),

(6) Cholesterol metabolism regulated by CAMKK2-CREB signaling promotes castration-resistant prostate cancerCell reportsJune 24, 2025Lin C, Pulliam TL, Han JJ, Xu J, Recio CV, Wilkenfeld SR, Shi Y, Kushwaha M, Bench S, Ruiz E, et al.Illumina® Platforms,Roche,KK8722 NEBNext® Ultra™II RNA Library Prep with Sample Purification Beads,NEB,E7775S NEBNext® Poly(A) mRNA Magnetic Isolation Module,Roche,E7490S Aurum™ Total RNA Mini Kit,Bio-rad,7326820 SuperScript™ III First-Strand Synthesis System

(7) Insights into global transcriptomic profile of biofilm producing Staphylococcus aureus clinical isolates from chronic foot ulcersIranian Journal of MicrobiologyMay 8, 2025Kumar Ebineshan, Aparna Srikantam, Michael Sukumar Pallapatidepletion, library preparation, quantification. NEBNext Ultra II RNA Library Prep Kit (Catalog: Catalog: E7775S, New England Biolabs) for Illumina was used for the library preparation. The enriched transcriptome

(8) The TRPM5 Antagonist Triphenylphosphine Oxide Increases Sebaceous Lipogenesis and Modulates Immune Phenotype of Human Sebocytes in a TRPM5-Independent MannerExp DermatolMay 7, 2025Dorottya Ádám, József Arany, Kinga Fanni Tóth, Orsolya Pető, Tamara Nyitrai, Balázs István Tóth, Szilárd Póliska, Christos C. Zouboulis, Attila Oláhprocess. RNA‐Seq libraries were prepared from total RNA using Ultra II RNA Sample Prep kit (Cat. No. E7775S; New England BioLabs; Ipswich, MA, USA) according to the manufacturer's protocol. Briefly, poly‐A RNAs

(9) UHRF1-mediated epigenetic reprogramming regulates glycolysis to promote progression of B-cell acute lymphoblastic leukemiaCell Death DisApril 29, 2025Yan Huang, Luting Luo, Yangqi Xu, Jiazheng Li, Zhengjun Wu, Chenxing Zhao, Jingjing Wen, Peifang Jiang, Haojie Zhu, Lingyan Wang, et al.of total RNA was used for library preparation with the NEBNext Ultra II RNA Library Prep Kit (Cat.#E7775S, New England Biolabs, Ipswich, USA) designed for Illumina sequencing. The raw data in fastq format were

(10) Defining gastric cancer ecology: the crucial roles of TREM2(+) macrophages and fibroblasts in tumor microenvironmentsCommunications biologyMarch 28, 2025Cao Q, Sun D, Tu C, Wang J, Fu R, Gong R, Xiao Y, Liu Q, Li X.employed to deplete ribosomal RNA (rRNA) from total RNA. The NEB Next Ultra RNA Library Prep Kit (#E7775S, NEB, USA) was used for Illumina library construction. Subsequently, RNA was fragmented into ~300 base

(11) Deciphering the dose-dependent effects of thymoquinone on cellular proliferation and transcriptomic changes in A172 glioblastoma cellsPLoS OneJanuary 28, 2025Rachana Pandey, Purushothaman Natarajan, Umesh K. Reddy, Wei Du, Cristian Sirbu, Moussa Sissoko, Gerald R. HankinsNEBNext®Ultra™ RNA Library Prep Kit for Illumina® (New England Biolabs Inc., Ipswich, MA, USA, Cat #E7770S/L, #E7775S/L). The final library quality and insert size were determined using a bioanalyzer (Invitrogen, USA),

(12) SLC25A1 promotes lymph node metastasis of esophageal squamous cell carcinoma by regulating lipid metabolismInt J OncolJanuary 16, 2025Guoquan Zhang, Jingru Wu, Minghao Ji, Xiangyan Liu, Mo ShiNEBNext® Poly (A) mRNA Magnetic Isolation Module and NEBNext® Ultra™ II mRNA Library Prep kit (cat. no. NEB #E7770S/L, Cat. No. #E7775S/L, New England Biolabs, Inc.,) for Illumina® were used for mRNA isolation and library construction following

(13) Global loss of promoter–enhancer connectivity and rebalancing of gene expression during early colorectal cancer carcinogenesisNature CancerOctober 30, 2024Yizhou Zhu, Hayan Lee, Shannon White, Annika K. Weimer, Emma Monte, Aaron Horning, Stephanie A. Nevins, Edward D. Esplin, Kristina Paul, Gat Krieger, et al.were prepared from 200 ng–1 μg of total RNA using the NEBNext Ultra unstranded preparation kit (NEB, E7775S and E7490S), in accordance with the manufacturer’s protocol. Samples were sequenced on a NovaSeq S1

(14) Growth deficiency in a mouse model of Kabuki syndrome 2 bears mechanistic similarities to Kabuki syndrome 1PLOS GeneticsJune 10, 2024Christine W. Gao, WanYing Lin, Ryan C. Riddle, Sheetal Chopra, Jiyoung Kim, Leandros Boukas, Kasper D. Hansen, Hans T. Björnsson, Jill A. Fahrnersample was used as input for the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (E7775S), in combination with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England BioLabs E7490L).

(15) A mouse model of Weaver syndrome displays overgrowth and excess osteogenesis reversible with KDM6A/6B inhibitionJCI InsightJanuary 9, 2024Christine W. Gao, WanYing Lin, Ryan C. Riddle, Priyanka Kushwaha, Leandros Boukas, Hans T. Björnsson, Kasper D. Hansen, Jill A. Fahrnerusing the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (New England BioLabs, E7775S) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs,

(16) RNAi-dependent expression of sperm genes in ADL chemosensory neurons is required for olfactory responses in Caenorhabditis elegansFrontiers in molecular biosciencesJanuary 1, 2024Ow MC, Nishiguchi MA, Dar AR, Butcher RA, Hall SE.NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB E7490S) and NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads (NEB E7775S) following the recommendations of the manufacturer. Indexing of libraries was done using NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1

(17) Gene expression signatures associated with chronic endometritis revealed by RNA sequencingFrontiers in MedicineJuly 20, 2023Kyoko Oshina, Keiji Kuroda, Kazuhiko Nakabayashi, Junko Tomikawa, Mari Kitade, Rikikazu Sugiyama, Kenichiro Hata, Atsuo ItakuraNEBNext rRNA Depletion Kit v2 (E7405L, NEB) and NEBNext Ultra II Directional RNA Library Prep Kit (E7775S, NEB), according to the manufacturer’s protocols with 1 μg of total RNA as starting material and with

(18) Novel mouse model of Weaver syndrome displays overgrowth and excess osteogenesis reversible with KDM6A/6B inhibitionbioRxivJuly 7, 2023Christine W Gao, WanYing Lin, Ryan C Riddle, Priyanka Kushwaha, Leandros Boukas, Hans T Björnsson, Kasper D Hansen, Jill A FahrnerLibraries were prepared using the NEBNext Ultra II RNA Library Prep kit with Sample Purification Beads (E7775S) and indexed with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1) (New England BioLabs

(19) Novel Insights to Assess Climate Resilience in Goats Using a Holistic Approach of Skin-Based Advanced NGS TechnologiesInternational Journal of Molecular SciencesJune 19, 2023Silpa Mullakkalparambil Velayudhan, Veerasamy Sejian, Chinnasamy Devaraj, Gundallahalli Bayyappa Manjunathareddy, Wilfred Ruban, Vinod Kadam, Sven König, Raghavendra Bhattafor the library preparation using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (Catalog: E7775S, New England Biolabs). The library concentration was determined in a Qubit.3 Fluorometer (Catalog: Q33216,

(20) Elucidating the role of c-di-AMP in Mycobacterium smegmatis: Phenotypic characterization and functional analysisHeliyonMay 1, 2023Vikas Chaudhary, Aditya Kumar Pal, Mamta Singla, Anirban Ghoshaccording to the manufacturer’s protocol. NEB Next Ultra II RNA Library Prep Kit (Catalog: Catalog: E7775S, New England Biolabs) for Illumina was used for the library preparation. The enriched transcriptome

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NEBNextUltraII RNA Library Prep with Sample Purification Beads

NEBNextUltraII RNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII RNA Library Prep with Sample Purification Beads from New England Biolabs

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