NEBNextUltraII DNA Library Prep with Sample Purification Beads E7103L from New England Biolabs

Product Specs
ItemNEBNextUltraII DNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $2,514.00 Inquire
Catalog NumberE7103L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Ubiquitin-dependent recruitment of SLFN11 to chromatin is regulated by deubiquitinase (DUB) and RNF168ResearchSquareApril 10, 2026Ukhyun Taniyama, Gianluca Pegoraro, Gianluca Pegoraro, Shar-Yin Naomi Huang, Shar-Yin Thomas, Laurent Thomas, Laurent Ozbun, Liton K Saha, Junko Murai, Ukhyun Murai, et al.Illumina adapters using the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs, #E7103L and #E6440). Libraries were quantified using a TapeStation system (Agilent Technologies) and sequenced

(2) CTCF/cohesin-binding sites are susceptible to replication-associated DNA damage and genomic instability in cancer cellsiScienceFebruary 20, 2026Elangoli Ebrahimkutty Faseela, Dimple Notani, Radhakrishnan SabarinathanNCBS. Library preparation was done using the NEBNextⓇ Ultra™ II DNA Library preparation kit (NEB, #E7103L). Libraries were sequenced on single-end 50 bp (1x50 bp) reads on the HiSeq 2500 Illumina platform.

(3) EZH2 directs HER2+ breast cancer progression through the modulation of epithelial plasticityEMBO ReportsJanuary 28, 2026Linshan Liu, Ellie J Massey, Dongmei Zuo, Alain Pacis, Mert Demirdizen, Elizabeth Podleszanski, Jessica Cinkornpumin, Yu Gu, Hailey Proud, Virginie Sanguin-Gendreau, et al.for library preparation. Libraries were generated with NEBNext Ultra II DNA Library Prep kits (NEB, E7103L) and sequenced at ***SickKids TCAG facilities. Analysis of publicly available datasets EZH2 expression

(4) IP-SNPs-seq links noncoding risk alleles to lineage transcription factor programs in prostate cancerCell Mol Life SciDecember 8, 2025Wenjie Xu, Qixiang Zhang, Lijuan Qiao, Zixi Wang, Tian Wang, Dandan Dong, Qin Zhang, Liang Wang, Gong-Hong Wei, Peng Zhang(28006, Qiagen). ChIP-seq libraries were constructed with the NEBNext Ultra II DNA Library Prep Kit (E7103L, NEB) following the manufacturer’s protocol. Sequencing was carried out by Annoroad Company. Libraries

(5) Combined SNPs sequencing and allele specific proteomics capture reveal functional causality underpinning the 2p25 prostate cancer susceptibility locusNat CommunOctober 8, 2025Dandan Dong, Zixian Wang, Mengqi Liu, Qin Zhang, Wenjie Xu, Yu Wei, Jing Zhu, Xiayun Yang, Qixiang Zhang, Yao Zhu, et al.Supplementary Table 2. ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (#E7103L, NEB) according to the manufacturer’s instructions. Sequencing was performed at Annoroad company. The

(6) Engineering precision zebrafish alleles of human diseasebioRxivMay 24, 2025Holly R. Thomas, Bradley K. Yoder, Matthew S. Alexander, John M. Parantfor sample indexing with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs E7103L) in preparation for next-generation sequencing (NGS) using Illumina NEBNext Multiplex Oligos indexing

(7) Dynamic activation of rAAV transgene expression by a small molecule that recruits endogenous transcriptional machineryNucleic Acids ResApril 29, 2025Sara R Wasserman, Savannah Muron, Hae Rim Lee, Madison L Routh, Austin J Hepperla, Deena M Scoville, Avery Huber, Jessica D Umana, Nicole E Pereira, Caroline A Foley, et al.using the NEBNext Ultra II DNA Library Prep with Sample Purification Beads kit (New England Biolabs E7103L) and NEBNext Multiplex Oligos for Illumina (E6440L). Bead based size selection was conducted by a 28

(8) Distinct structural and functional heterochromatin partitioning of lamin B1 and lamin B2 revealed using genome-wide nicking enzyme epitope targeted DNA sequencingNucleic Acids ResJanuary 16, 2025Sagnik Sen, Pierre-Olivier Estève, Karthikeyan Raman, Julie Beaulieu, Hang Gyeong Chin, George R Feehery, Udayakumar S Vishnu, Shuang-Yong Xu, James C Samuelson, Sriharsa Pradhan50 μl of low TE buffer, and DNA libraries were made using NEBNext Ultra II DNA Library Prep (NEB # E7103L) with some modifications. Briefly, end repair/dA tailing was performed on size selected DNA and NEBNext

(9) Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodelerNature CommunicationsSeptember 6, 2024Avik Kumar Pal, Vivek Hari-Sundar Gandhivel, Amruta B. Nambiar, P. V. Shivaprasadfor library preparation using NEBNext® Ultra™ II DNA Library Prep Kit using purification beads (NEB, E7103L) as per manufacturer’s protocol. The libraries were sequenced on NovaSeq 6000 platform (2 × 100 bp mode). The

(10) Systematic dissection of sequence features affecting binding specificity of a pioneer factor reveals binding synergy between FOXA1 and AP-1Molecular CellAugust 8, 2024Cheng Xu, Holly Kleinschmidt, Jianyu Yang, Erik M. Leith, Jenna Johnson, Song Tan, Shaun Mahony, Lu Bailocus-specific primer pairs. Sequencing library was constructed by NEBNext ultra II DNA library prep kit (NEB E7103L). 50 million paired-end 50bp reads were obtained for each ChIP and input sample using a NextSeq 2000

(11) SIKs Regulate HDAC7 Stabilization and Cytokine Recall in Late-Stage T Cell Effector DifferentiationJournal of immunology (Baltimore, Md. : 1950)December 15, 2023Helms RS, Marin-Gonzalez A, Patel CH, Sun IH, Wen J, Leone RD, Duvall B, Gao RD, Ha T, Tsukamoto T, et al.prepared and amplified using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs Cat# E7103L) and dual-indexed eith the NEBNext Multiplex Oligos for Illumina (New England BioLabs Cat# E6440S).

(12) An HBV susceptibility variant of KNG1 modulates the therapeutic effects of interferons α and λ1 in HBV infection by promoting MAVS lysosomal degradationEBioMedicineAugust 1, 2023Zhang B, Han H, Zhao X, Li AN, Wang Y, Yuan W, Yang Z, Li MD.preparation. Sequencing libraries were generated using NEBNext® Ultra™ DNA Library Prep Kit for Illumina (#E7103L, NEB, USA) according to the manufacturer’s recommendations, and index codes were used to attribute sequences

(13) Intrinsic mesoscale properties of a Polycomb protein underpin heterochromatin fidelityNature Structural & Molecular BiologyJuly 1, 2023Sujin Lee, Stephen Abini-Agbomson, Daniela S. Perry, Allen Goodman, Beiduo Rao, Manning Y. Huang, Jolene K. Diedrich, James J. Moresco, John R. Yates, Karim-Jean Armache, et al.overnight at 4 °C. Library preparation was carried out using NEBNext Ultra II DNA Library Prep Kit (NEB E7103L). ChIP-seq analysis Sequencing reads were trimmed for adaptor sequence (GATCGGAAGA) using Cutadapt52

(14) Plant polymerase IV sensitizes chromatin through histone modifications to preclude spread of silencing into protein-coding domainsGenome ResearchJune 5, 2023Vivek Hari Sundar G, Chenna Swetha, Debjani Basu, Kannan Pachamuthu, Steffi Raju, Tania Chakraborty, Rebecca A. Mosher, P.V. Shivaprasadlibrary preparation using NEBNext Ultra II DNA library prep kit with sample purification beads (NEB E7103L) as per the manufacturer's protocol. The libraries (with replicates) were sequenced on a Illumina HiSeq

(15) Single duplex DNA sequencing with CODEC detects mutations with high sensitivityNature GeneticsMay 1, 2023Jin H. Bae, Ruolin Liu, Eugenia Roberts, Erica Nguyen, Shervin Tabrizi, Justin Rhoades, Timothy Blewett, Kan Xiong, Gregory Gydush, Douglas Shea, et al.IDT. End-repair/dA-tailing Unless noted otherwise, we used NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7103L) for ER/AT. Duplex-Repair32 consists of four steps. In step 1, DNA is treated with an enzyme cocktail

(16) Epigenetic centromere identity is precisely maintained through DNA replication but is uniquely specified among human cellsLife science allianceMarch 1, 2023Mahlke MA, Lumerman L, Ly P, Nechemia-Arbely Y.Kit. ChIP-seq library generation and sequencing ChIP libraries were prepared using NEB Ultra II (Cat # E7103L), following NEBNext protocols with minor modifications. To reduce biases induced by PCR amplification

(17) Dual-specificity RNA aptamers enable manipulation of target-specific O-GlcNAcylation and unveil functions of O-GlcNAc on β-cateninCellJanuary 19, 2023Zhu Y, Hart GW.Q33230). Preparation of Sequencing Library was performed using the NEBNext Ultra II DNA Library Prep Kit (NEB E7103L), and NEBNext Multiplex Oligos for Illumina (NEB E6440S), following the protocol by Liu94. 6 ng DNA

(18) Intronic enhancers of the human SNCA gene predominantly regulate its expression in brain in vivoScience advancesNovember 25, 2022Cheng F, Zheng W, Liu C, Barbuti PA, Yu-Taeger L, Casadei N, Huebener-Schmid J, Admard J, Boldt K, Junger K, et al.used for the library preparation using the NEB Ultra II DNA Library Preparation Kit for Illumina (NEB, E7103L). Next-generation sequencing was done on an Illumina NextSeq500/NovaSeq 6000 system using the 75/100-bp

(19) VHL Ser65 mutations enhance HIF2α signaling and promote epithelial-mesenchymal transition of renal cancer cellsCell & bioscienceMay 3, 2022Ma X, Tan Z, Zhang Q, Ma K, Xiao J, Wang X, Wang Y, Zhong M, Wang Y, Li J, et al.purification kit (Qiagen) and subjected to library preparation by NEBNext® Ultra™ II DNA Library Prep Kit (E7103L, NEB). A NovaSeq 6000 S4 sequencing system (Illumina, San Diego, CA, USA) was used to sequence the DNA

(20) EZH2 noncanonically binds cMyc and p300 through a cryptic transactivation domain to mediate gene activation and promote oncogenesisNature cell biologyMarch 1, 2022Wang J, Yu X, Gong W, Liu X, Park KS, Ma A, Tsai YH, Shen Y, Onikubo T, Pi WC, et al.DNA purification. Multiplexed ChIP-seq libraries were prepared by using NEBNext Ultra II kit (NEB, #E7103L) and sequenced on the Nextseq 500 system using Nextseq 550 High Output Kit v2.5 (Illumina). ChIP-seq

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NEBNextUltraII DNA Library Prep with Sample Purification Beads

NEBNextUltraII DNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII DNA Library Prep with Sample Purification Beads from New England Biolabs

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