HiScribe SP6 RNA Synthesis Kit E2070S from New England Biolabs

Product Specs
ItemHiScribe SP6 RNA Synthesis Kit
CompanyNew England Biolabs
Price $298.00 Inquire
Catalog NumberE2070S
Quantity50 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) ACRC/GCNA is an essential protease that repairs DNA-protein crosslinks during vertebrate developmentNucleic Acids ResApril 20, 2026Cecile Otten, Marin Kutnjak, Christine Supina-Pavic, Marija Pranjic, Ivan Anticevic, Vanna Medved, Marta Popoviclinearized plasmid by performing an in vitro transcription with SP6 using the Hiscribe SP6 RNA kit (#E2070S, NEB) and a cap analog from the ARCA kit (#S1411, NEB) for improved mRNA stability. The resulting mRNA

(2) U2AF regulates the translation and localization of nuclear-encoded mitochondrial mRNAsMol CellApril 2, 2026Gloria R. Garcia, Murali Palangat, Josquin Moraly, Benjamin T. Donovan, Bixuan Wang, Ira Phadke, David Sturgill, Jiji Chen, Guofeng Zhang, Ronald J. Holewinski, et al.RNA cleanup kit,NEB,T2040L DNA Clean & Concentrator Kit,Zymo,D4003 HiScribe SP6 RNA synthesis kit,NEB,E2070S Nano-Glo Luciferase Assay,Promega,N1110 MitoTracker Red CMXRos,Invitrogen,2351994 FoxP3 Staining

(3) Genetically tractable embryonic cell lines from sea urchins Lytechinus variegatus and Strongylocentrotus purpuratusCommun BiolOctober 14, 2025Kate R. Castellano, Carl J. Manner, Riss M. Kell, Reanna M. McAtee, Nicole M. Capozzi, Gregory A. Wray, Andrea G. Bodnarused as a DNA template for in vitro transcription using the HiScribe SP6 Kit (New England Biolabs, E2070S) and capped with a 3′-O-Me-m7G(5’)ppp(5’)G RNA cap structure analog (New England Biolabs, S1411S) following

(4) In vivo detection of antisense HIV-1 transcripts in untreated and ART-treated individualsLife Sci AllianceJuly 14, 2025Adam A Capoferri, Rachel Sklutuis, Toluleke O Famuyiwa, Sachi Pathak, Rui Li, Jason W Rausch, Brian T Luke, Rebecca Hoh, Steven G Deeks, John W Mellors, et al.Kit (#E2050S; New England Biolabs) for AST or the HiScribe SP6 Quick High Yield RNA Synthesis Kit (#E2070S; New England Biolabs) for env transcripts. Transcribed RNA was purified using a Monarch RNA Cleanup

(5) Biallelic variants in the conserved ribosomal protein chaperone gene PDCD2 are associated with hydrops fetalis and early pregnancy lossProceedings of the National Academy of SciencesApril 15, 2025Anne-Marie Landry-Voyer, Tess Holling, Emily K. Mis, Zabih Mir Hassani, Malik Alawi, Weizhen Ji, Lauren Jeffries, Kerstin Kutsche, François Bachand, Saquib A. Lakhanitranscribed using T7 HiScribe RNA Synthesis Kit (E2040S—antisense) or Sp6 HiScribe RNA Synthesis Kit (E2070S—sense) from New England Biolabs. Embryos were collected at the desired stages, fixed in MEMFA (3-(N-morpholino)

(6) The Hydractinia cell atlas reveals cellular and molecular principles of cnidarian colonialityNat CommunMarch 3, 2025David A. Salamanca-Díaz, Helen R. Horkan, Helena García-Castro, Elena Emili, Miguel Salinas-Saavedra, Alberto Pérez-Posada, Maria Eleonora Rossi, Marta Álvarez-Presas, Rowan Mac Gabhann, Paula Hillenbrand, et al.RNA polymerases according to the manufacturer’s protocol (NEB HiScribe T7, E2040S or SP6 High Yield, E2070S, RNA Synthesis Kits). Roche DIG-labelling and detection kit (Roche, 11093657910) was used. In situ hybridisation

(7) Design and Expression of Fasciola hepatica Multiepitope Constructs Using mRNA Vaccine TechnologyInternational Journal of Molecular SciencesJanuary 30, 2025Javier Sánchez-Montejo, Tania Strilets, Raúl Manzano-Román, Julio López-Abán, Mariano A. García-Blanco, Belén Vicente, Antonio Muroreaction was then carried out with 500 ng of PCR product using the HiScribe SP6 RNA Synthesis Kit (E2070S, NEB, Ipswich, MA, USA), following the manufacturer’s instructions and using the same conditions used

(8) Nup107 contributes to the maternal to zygotic transition by preventing the premature nuclear export of pri-miRNA 427DevelopmentJanuary 15, 2025Valentyna Kostiuk, Rakib Kabir, Kaitlin Levangie, Stefany Empke, Kimberly Morgan, Nick D. L. Owens, C. Patrick Lusk, Mustafa K. Khokhaprobes were generated using Sp6 and T7, respectively, with the HiScribe High Yield RNA Synthesis Kit (E2070S and E2050S) from New England Biolabs according to the manufacturer's instructions. Plasmids used for

(9) Mechanistic insights into Sindbis virus infection: noncapped genomic RNAs enhance the translation of capped genomic RNAs to promote viral infectivityNucleic Acids ResearchJanuary 7, 2025Deepa Karki, Autumn T LaPointe, Cierra Isom, Milton Thomas, Kevin J Sokoloskiμg of linearized plasmid was used as a template for transcriptions with SP6 RNA polymerase (Catalog #E2070S; New England Biolabs) in a total volume of 50 μl containing 5 mM of each rNTP (ATP, CTP, UTP and GTP)

(10) miRNA-target complementarity in cnidarians resembles its counterpart in plantsEMBO ReportsJanuary 2, 2025Yael Admoni, Arie Fridrich, Paris K Weavers, Reuven Aharoni, Talya Razin, Miguel Salinas-Saavedra, Michal Rabani, Uri Frank, Yehu Moran,Zymo Research,R1013 SapI restriction enzyme,New England Biolabs,R0569S HiScribe SP6 RNA synthesis kit,New England Biolabs,E2070S Protein A Magnetic Beads,MedChem Express,HY-K0203 Protease inhibitor cOmplete ULTRA tablets,Merck Millipore

(11) A novel N 4, N 4-dimethylcytidine in the archaeal ribosome enhances hyperthermophilyProceedings of the National Academy of SciencesNovember 5, 2024Kristin A. Fluke, Nan Dai, Eric J. Wolf, Ryan T. Fuchs, P. Shing Ho, Victoria Talbott, Liam Elkins, Yueh-Lin Tsai, Jackson Schiltz, Hallie P. Febvre, et al.nt, 300 nt, 400, and 966 nt) were generated by in vitro transcription using an Sp6 system (NEB cat# E2070S) according to the manufacturer’s protocol. RNA pellets were suspended in nuclease-free water and stored

(12) Enhancing CRISPR prime editing by reducing misfolded pegRNA interactionseLifeJune 7, 2024Weiting Zhang, Karl Petri, Junyan Ma, Hyunho Lee, Chia-Lun Tsai, J Keith Joung, Jing-Ruey Joanna YehpegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs, no. T2030L) and eluted in water. Purification

(13) Harnessing noncanonical crRNA for highly efficient genome editingNature CommunicationsMay 7, 2024Guanhua Xun, Zhixin Zhu, Nilmani Singh, Jingxia Lu, Piyush K. Jain, Huimin Zhaofollowing program: 98 °C 5 min, 0.1 °C/s cool down to 4 °C. HiScribeTM SP6 RNA Synthesis Kit (NEB, E2070S) was used for IVT reactions according to the manufacturer’s protocol. To generate Z-incorporated crRNA,

(14) Programmable modulation of ribosomal frameshifting by mRNA targeting CRISPR-Cas12a systemiScienceDecember 15, 2023Shih-Hong Huang, Shih-Cheng Chen, Tsu-Ying Wu, Cheng-Yao Chen, Chien-Hung YuE5520S T7 RNA Polymerase,New England Biolabs,Cat# E2040S SP6 RNA Polymerase,New England Biolabs,Cat# E2070S Anti-Reverse Cap Analog (ARCA),New England Biolabs,Cat# S1411S EasyTag L-[35S]-Methionine,Perkin Elmer,Cat#

(15) Enhancing CRISPR prime editing by reducing misfolded pegRNA interactionsbioRxivAugust 20, 2023Weiting Zhang, Karl Petri, Junyan Ma, Hyunho Lee, Chia-Lun Tsai, J. Keith Joung, Jing-Ruey Joanna YehpegRNAs and gRNAs was performed using the HiScribe SP6 RNA Synthesis Kit (New England Biolabs, no. E2070S), purified using the Monarch RNA Cleanup Kit (New England Biolabs, no. T2030L) and eluted in water. Purification

(16) CRISPR prime editing with ribonucleoprotein complexes in zebrafish and primary human cellsNature biotechnologyFebruary 1, 2022Petri K, Zhang W, Ma J, Schmidts A, Lee H, Horng JE, Kim DY, Kurt IC, Clement K, Hsu JY, et al.HiScribe™ T7 High Yield RNA synthesis Kit (New England Biolabs, E2040S) or HiScribe™ SP6 RNA Synthesis Kit (New England Biolabs, E2070S) and purified using the Monarch® RNA Cleanup Kit (New England Biolabs, T2030L). Comparison of the editing

(17) EHD2 modulates Dll4 endocytosis during blood vessel developmentMicrocirculation (New York, N.Y. : 1994)January 1, 2022Webb AM, Francis CR, Judson RJ, Kincross H, Lundy KM, Westhoff DE, Meadows SM, Kushner EJ.scaffold were synthesized for each target gene using HiScribe™ SP6 RNA Synthesis Kit (New England BioLabs, E2070S). Injection mixture was prepared on ice containing 5 µM Cas9 (PNA Bio, CP02), 1 µg/µl sgRNA, and brought

(18) Maximizing transcription of nucleic acids with efficient T7 promotersCommunications BiologyDecember 1, 2020Thomas Conrad, Izabela Plumbom, Maria Alcobendas, Ramon Vidal, Sascha Sauermix and 7.5 mM each of GTP, ATP, CTP, and UTP from the HiScribe T7/SP6 RNA Synthesis Kit (NEB E2040S, E2070S). After 2 h incubation, RNA was purified with 1.6 volumes RNAClean XP beads (Beckman Coulter), and residual

(19) Hox and Wnt pattern the primary body axis of an anthozoan cnidarian before gastrulationNature CommunicationsDecember 1, 2018Timothy Q. DuBuc, Thomas B. Stephenson, Amber Q. Rock, Mark Q. Martindalefollowed by in vitro transcription (NEB Highscribe™ T7 and Sp6 RNA synthesis kits; Cat# E2040S and E2070S), and RNA purification (Zymogen RNA Clean and Concentrator™-25; Cat# R1017). gRNAs at a final concentration

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HiScribe SP6 RNA Synthesis Kit

HiScribe SP6 RNA Synthesis Kit from New England Biolabs

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New England Biolabs

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HiScribe SP6 RNA Synthesis Kit from New England Biolabs

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