NEBNext Single Cell/Low Input cDNA Synt E6421L from New England Biolabs

Product Specs
ItemNEBNext Single Cell/Low Input cDNA Synt
CompanyNew England Biolabs
Price $2,288.00 Inquire
Catalog NumberE6421L
Quantity96 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cellsSTAR ProtocolsMarch 20, 2026Anita Adami, Raquel Garza, Fereshteh Dorazehi, Christopher H. Douse, Johan JakobssonCell/Low Input cDNA Synthesis & Amplification Module,New England Biolabs,Cat#: E6421S for 24 reactions or E6421L for 96 reactions Bioanalyzer High Sensitivity kit,Agilent,NA ,, Deposited data,Deposited data,Deposited

(2) High-altitude de-acclimatization and long-term immune suppression: the role of Nrf2 in Treg function targeting 3PMEPMA JMarch 4, 2026Yuxin Wang, Zhijie Bai, Jiamiao Li, Jinfeng Liu, Yunan Zhang, Xizheng Wang, Lei Zhou, Haoyu Ni, Pan Shen, Ningning Wang, et al.was performed using the NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, E6421L, E7805, E6440) according to the manufacturer’s instructions. The libraries were kept at low temperature

(3) Domestication shaped the chromatin landscape of grain amaranthNature CommunicationsNovember 21, 2025Corbinian Graf, Tom S. Winkler, Peter J. Maughan, Markus G. Stettercomplementary DNA (cDNA) using a NEBNext® single cell/low input cDNA synthesis and amplification kit (E6421L), which uses a template switching method to generate full-length cDNAs (New England BioLabs, Ipswich,

(4) High-resolution genome assembly and linkage mapping in Meloidogyne hapla reveal non-canonical telomere repeats and recombination hotspots associated with effector proteinsPLOS PathogensNovember 18, 2025Pallavi Shakya, Muhammad I. Maulana, Etienne G. J. Danchin, M. Laurens Voogt, Stefan J. S. van de Ruitenbeek, Jacinta Gimeno, Adam P. Taranto, Alison C. Blundell, Evelin Despot-Slade, Nevenka Meštrović, et al.Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, Ipswich, MA, Cat. #E6421L). After fifteen cycles of PCR for cDNA amplification, the cDNA was purified using 0.86X SMRTbell cleanup

(5) LINE-1 retrotransposons mediate cis-acting transcriptional control in human pluripotent stem cells and regulate early brain developmentCell GenomicsOctober 8, 2025Anita Adami, Raquel Garza, Patricia Gerdes, Pia A. Johansson, Fereshteh Dorazehi, Symela Koutounidou, Laura Castilla-Vallmanya, Diahann A.M. Atacho, Yogita Sharma, Jenny G. Johansson, et al.Cell/Low Input cDNA Synthesis & Amplification Module,New England Biolabs,Cat#: E6421S for 24 reactions or E6421L for 96 reactions Qubit dsDNA HS kit,Invitrogen(ThermoFisher),Cat# Q32851 Bioanalyzer High Sensitivity

(6) A pangenome reveals LTR repeat dynamics as a major driver of genome evolution in ChenopodiumPlant GenomeFebruary 28, 2025Kate E. Jaggi, Karol Krak, Helena Štorchová, Bohumil Mandák, Ashley Marcheschi, Alexander Belyayev, Eric N. Jellen, John Sproul, David Jarvis, Peter J. Maughancomplementary DNA (cDNA) using a NEBNext single cell/low input cDNA synthesis and amplification kit (E6421L) which uses a template switching method to generate full‐length cDNAs (New England BioLabs). IsoSeq

(7) Genomes of Aegilops umbellulata provide new insights into unique structural variations and genetic diversity in the U‐genome for wheat improvementPlant Biotechnology JournalDecember 1, 2024Jatinder Singh, Santosh Gudi, Peter J. Maughan, Zhaohui Liu, James Kolmer, Meinan Wang, Xianming Chen, Matthew N. Rouse, Pauline Lasserre‐Zuber, Héléne Rimbert, et al.complementary DNA (cDNA) using a NEBNext® single cell/low input cDNA synthesis and amplification kit (E6421L) which uses a template switching method to generate full length cDNAs (New England BioLabs, Ipswich,

(8) Functional and dynamic profiling of transcript isoforms reveals essential roles of alternative splicing in interferon responseCell GenomicsOctober 9, 2024Mahoko Takahashi Ueda, Jun Inamo, Fuyuki Miya, Mihoko Shimada, Kensuke Yamaguchi, Yuta KochiKit,QIAGEN,Cat#74104 NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module,New England Biolabs,Cat# E6421L ProNex Size-Selective Purification System,Promega Corporation,Cat# NG2001 SMRTbell Express Template

(9) Long-read de novo genome assembly of Gulf toadfish (Opsanus beta)BMC GenomicsSeptember 18, 2024Nicholas S. Kron, Benjamin D. Young, Melissa K. Drown, M. Danielle McDonaldwas constructed using a NEBNext® Single Cell/Low Input kit (New England Biolabs, Ipswich, MA; Cat. #E6421L) with 500 ng of total RNA as input. Resulting cDNA was amplified for 15 cycles using the cDNA Synthesis

(10) North American pitseed goosefoot (Chenopodium berlandieri) is a genetic resource to improve Andean quinoa (C. quinoa)Scientific ReportsMay 29, 2024Peter J. Maughan, David E. Jarvis, Eulogio de la Cruz-Torres, Kate E. Jaggi, Heather C. Warner, Ashley K. Marcheschi, H. Daniel Bertero, Luz Gomez-Pando, Francisco Fuentes, Mayela E. Mayta-Anco, et al.prepared from the bulk sample using a NEBNext® single cell/low input cDNA synthesis and amplification kit (E6421L; New England BioLabs, Ipswich, MA). IsoSeq libraries were prepared using the SMRTbell v3.0 library prep

(11) LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversificationScience AdvancesNovember 3, 2023Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski, et al.Single Cell/Low Input cDNA Synthesis & Amplification Module (catalog nos. E6421S for 24 reactions and E6421L for 96 reactions, New England Biolabs), the Iso-Seq Express Oligo Kit (catalog no. PN-101737500, PacBio),

(12) Mitochondrial network adaptations of microglia reveal sex-specific stress response after injury and UCP2 knockoutiScienceOctober 20, 2023Maes ME, Colombo G, Schoot Uiterkamp FE, Sternberg F, Venturino A, Pohl EE, Siegert S.assays ,, NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module,New England BioLabs,Cat#: E6421L Luna® Universal qPCR Master Mix,New England BioLabs,Cat#: M3003L ,, Experimental models: organisms/strains,Experimental models

(13) Bat pluripotent stem cells reveal unusual entanglement between host and virusesCellMarch 2, 2023Déjosez M, Marin A, Hughes GM, Morales AE, Godoy-Parejo C, Gray JL, Qin Y, Singh AA, Xu H, Juste J, et al.cDNA synthesis using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, E6421L), which employs a modified oligodT primer and template switching technology to reverse-transcribe full-length

(14) Reference long-read isoform-aware transcriptomes of 4 human peripheral blood lymphocyte subsetsG3 (Bethesda, Md.)November 4, 2022Woolley CR, Chariker JH, Rouchka EC, Ford EE, Hudson EA, Waigel SJ, Smith ML, Mitchell TC.primed cDNA synthesis using commercially available NEB Next reagents (New England Biolabs, cat no. E6421L). Barcoded primers were incorporated into the cDNA during second-strand synthesis. Following double-stranded

(15) Identification of Alternative Polyadenylation in Cyanidioschyzon merolae Through Long-Read Sequencing of mRNAFrontiers in GeneticsJanuary 28, 2022Leonard Schärfen et al.cDNA synthesis using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, #E6421L), which employs a modified oligodT primer and template switching technology to reverse-transcribe full-length

(16) RNase H-dependent PCR-enabled T Cell Receptor sequencing (rhTCRseq) for Highly Specific and Efficient Targeted Sequencing of T Cell Receptor mRNA for Single-Cell and Repertoire AnalysisNature ProtocolsAugust 1, 2019Shuqiang Li et al.reagents from the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs E6421L). Steps 1A(x-xvii) are essentially the same as for Smart-seq215,16: a RT reaction with an oligo dT primer

(17) Neoantigen vaccine generates intratumoral T cell responses in phase Ib glioblastoma trialNatureJanuary 1, 2019Keskin DB, Anandappa AJ, Sun J, Tirosh I, Mathewson ND, Li S, Oliveira G, Giobbie-Hurder A, Felt K, Gjini E, et al.using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs E6421L). The protocol uses RNase H-dependent polymerase chain reaction (rhPCR) technology to improve the specificity

Be the First to Write a Review!

NEBNext Single Cell/Low Input cDNA Synt

NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs

Description

New England Biolabs

Citations (17)

Reviews

NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map