NEBNextUltraII RNA Library Prep with Sample Purification Beads E7775L from New England Biolabs

Product Specs
ItemNEBNextUltraII RNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $3,417.00 Inquire
Catalog NumberE7775L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Global genomic diversity of the selfing nematode Caenorhabditis tropicalis correlates with geographybioRxivApril 8, 2026Bowen Wang, Nicolas D. Moya, Robyn E. Tanny, Michael E.G. Sauria, Lance M. O'Connor, Ayeh Khorshidian, Ryan McKeown, Lewis Stevens, Claire Buchanan, Timothy A. Crombie, et al.NEBNext Ultra II RNA Library Prep Kit with Sample Purification Beads (New England Biolabs, catalog no. E7775L). The resulting cDNA library was adapter-ligated using adapters and unique dual indexes from the NEBNext

(2) LINE-1 Locus Transcription Nucleates Oncogenic Chromatin ArchitectureCancer DiscoveryApril 1, 2026Michael Lee, Yuannyu Zhang, Jun Yi Stanley Lim, Tao Dai, James Ye, Margaret B. Cervantes, Varun Sondhi, Sisi Zheng, Yoon Jung Kim, Brandon Chen, et al.prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs, E7775L); RNA fragmentation steps were omitted with the chrRNA-seq samples to preserve transcript lengths. Paired-end

(3) Integrated analysis of GWAS and molQTLs reveals cell-specific genetic variants in the porcine immune systemCommunications BiologyFebruary 11, 2026Jinyan Yang, Siqian Chen, Yongjie Tang, Xini Wang, Lulu Wang, Huatao Liu, Fuping Ma, Qingyao Zhao, Kai Xing, Ying Yu, et al.RNA sequencing libraries were prepared using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775L) following the manufacturer’s instructions. The resulting libraries were sequenced on an Illumina NovaSeq X Plus

(4) Morphological, physiological, and transcriptomic analysis of Taxodium mucronatum under different salinity stressesFrontiers in Plant ScienceFebruary 4, 2026Yunpeng Gao, Hongling Wang, Shizheng Shi, Ruifang Huang, Liwen Liang, Jue Zhang, Kaipeng Jiang, Tao Huang, Shuxian Li, Cong Lei, et al.Strand-specific libraries were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (E7775L, New England Biolabs, USA) and indexed with NEBNext Multiplex Oligos (E7600S). Paired-end 150-bp sequencing

(5) Transcriptomics Reveals Cold Tolerance Maize Lines Involved in the Phenylpropanoid and Flavonoid PathwaysPlants (Basel)January 5, 2026Shuna Zhou, Xinling Yu, Jian Tan, Haixiao Sun, Wei Yang, Liangyu Jiang, Zhenyuan Zang, Jiabin Ci, Xuejiao Renprepared using the NEBNext Ultra II RNA Library Prep Kit (New England Biolabs, Ipswich, MA, USA, cat. no. E7775L) [39]. Libraries were quantified with Qubit 3.0 and sequenced on the Illumina NovaSeq 6000 platform

(6) Posterior enhancer (p-Enh) maintains early neuromesodermal progenitors bi-potency during gastrulationCell RegenerationNovember 15, 2025Panpan Mi, Yingying Chen, Fengxiang Tan, Penglei Shen, Yun Yang, Mingzhu Wen, Yun Qian, Jichang Wang, Naihe Jing, Xianfa Yangng of high-quality total RNA with the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, cat #E7775L). The resulting libraries underwent paired-end sequencing (150 bp read length) on an Illumina NovaSeq

(7) Characterization of Novel Luteoviruses in Canadian Highbush Blueberries Using High-Throughput SequencingVirusesSeptember 23, 2025Sachithrani Kannangara, Adam Gilewski, Juan Rodriguez Lopez, Gertruida de Villiers, Meghan Ellis, Peter Ellis, Eric Gerbrandt, Jim MattssonNEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA, USA, Cat. No. E7775L). Each cDNA library was tagged during PCR enrichment (11 cycles) with next generation sequencing (NGS)

(8) DBN1‑mediated upregulation of GAB2 facilitates the migration and invasion of T‑cell acute lymphoblastic leukemia cellsOncology ReportsSeptember 3, 2025Jiaxing Sun, Xiaoxing Huang, Xingruo Zeng, Yufei Lei, Hengjing He, Zimeng Wei, Di Xiao, Qiuping Zhang, Xinran Li, Fuling Zhou, et al.sample was used to generate an RNA-seq library using the NEBNext® UltraTM RNA Library Prep Kit (cat. no. E7775L; New England BioLabs, Inc.) according to the manufacturer's protocol. Briefly, mRNA with poly A was

(9) Gastrula‐Premarked Posterior Enhancer Primes Posterior Tissue Development Through Cross‐Talk with TGF‐β Signaling PathwayAdvanced ScienceSeptember 1, 2025Yingying Chen, Fengxiang Tan, Qing Fang, Lin Zhang, Jiaoyang Liao, Penglei Shen, Yun Qian, Mingzhu Wen, Rui Song, Yonggao Fu, et al.libraries were prepared from 300 ng pure total RNA using NEBNext Ultra II RNA Library Prep kit (NEB, E7775L). Two highly reproducible biological replicates (each with two technical replicates) were performed.

(10) Transcriptome Analysis of Developmental Gene Expression in Thesium chinense TurczPlants (Basel)August 16, 2025Sijia Liang, Qiongqiong Wang, Qin Han, Xinmin Zhang, Yiyuan Liu, Miaosheng Chen, Chengcai Zhang, Zhaoyang Wang, Junxiao Li, Di Yu, et al.library construction, the NEBNext® UltraTM II RNA Library Prep Kit for Illumina® (NEB, Ipswich, MA, USA, Cat# E7775L) was used according to the manufacturer’s instructions. The prepared libraries were sequenced on the

(11) XYLEM NAC DOMAIN 1 (EjXND1) relieves cold-induced lignification by negatively regulating the EjHB1-EjPRX12 module in loquat fruitJournal of Advanced ResearchJuly 1, 2025Zihao Liang, Yanna Shi, Yiqing Huang, Jiao Lu, Mengxue Zhang, Xizhi Cao, Ruoqian Hu, Dongdong Li, Wenbo Chen, Changqing Zhu, et al.Library construction was conducted using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775L). The library was sequenced using NovaSeq 6000 (Illumina, USA), with 151 sequencing cycles. Following

(12) Cancer type and gene signatures associated with breakthrough infections following COVID-19 mRNA vaccinationNPJ VaccinesMay 9, 2025Youjia Zhong, Jiaqi Li, Ainsley Ryan Yan Bin Lee, Janice Yu Jin Tan, Carina Jing Xuan Tay, Clara Wt Koh, Ethan Yong Tzi Seow, Wee Chee Yap, Shi Yin Wong, Chun En Yau, et al.(NEB, E7755X), followed by library construction using the NEBNext Ultra II RNA Library Prep Kit (NEB, E7775L). The library size and concentration were verified via HT DNA Extended Range LabChip (Perkin Elmer,

(13) TLR7/8 signaling activation enhances the potency of human pluripotent stem cell-derived eosinophils in cancer immunotherapy for solid tumorsExp Hematol OncolMarch 1, 2025Sheng Zhu, Zhengyang Zhou, Ruixin Gu, Zixin Zhao, Yingfeng Zhang, Yudi Miao, Qi Lei, Tianxing Liu, Guokai Wang, Chenyi Dai, et al.were constructed using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB, Rowley, MA; #E7775L). The fragmented paired-end libraries were sequenced using an Illumina NovaSeq 6000. All sequencing

(14) Remimazolam alleviates myocardial ischemia/reperfusion injury and inflammation via inhibition of the NLRP3/IL‑1β pathway in miceInternational Journal of Molecular MedicineJanuary 30, 2025Xueru Liu, Guojing Shui, Yan Wang, Tangting Chen, Peng Zhang, Li Liu, Chunhong Li, Tao Li, Xiaobin Wangtranscriptome sequencing with the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina (cat. no. NEB#E7775L; New England BioLabs, Inc.). Clustering of the index-coded samples was performed on a cBot Cluster Generation

(15) Transcriptome and Metabolome Analyses of Aroma Differences between Chardonnay and a Chardonnay Bud SportMoleculesAugust 2, 2024Xiaoqin Bao, Jin Dong, Min Niu, Zhilei Wang, Guoqian XuSequencing libraries were generated using NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775L). PCR was performed with random oligonucleotides as primers and the polymerase provided in the NEB kit.

(16) Preliminary studies on the molecular mechanism of intramuscular fat deposition in the longest dorsal muscle of sheepBMC GenomicsJune 12, 2024Xuwen Shao, Xintan Lu, Xinming Sun, Huaizhi Jiang, Yang ChencDNA library preparation and RNA-seq, The NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® (NEB #E7775L) was used. Get clean reads from raw data by removing low-quality reads and reads that contain adapters

(17) Integration of Metabolomic and Transcriptomic Analyses Reveals the Molecular Mechanisms of Flower Color Formation in Prunus mumePlants (Basel)April 11, 2024Ruyi Wang, Xin Yang, Tao Wang, Baohui Li, Ping Li, Qin Zhangthe sequencing strategy was PE150. A NEBNext® Ultra TM II RNA Library Prep Kit for Illumina® (NEB # E7775L) library construction kit was used to perform transcriptome sequencing and assembly of samples. The

(18) Pathophysiology of chikungunya virus infection associated with fatal outcomesCell host & microbeApril 10, 2024de Souza WM, Fumagalli MJ, de Lima STS, Parise PL, Carvalho DCM, Hernandez C, de Jesus R, Delafiori J, Candido DS, Carregari VC, et al.Nanopore Technologies,FLO-MIN106D NEBNext Ultra II Directional RNA Library Prep,New England Biolabs,E7775L AMPure XP Beads,Beckman Coulter,A63882 Quick-RNA MagBead Kit,Zymo Research,R2133 ZyQuik sPECAM-1

(19) Novel and improved Caenorhabditis briggsae gene models generated by community curationBMC GenomicsAugust 25, 2023Nicolas D. Moya, Lewis Stevens, Isabella R. Miller, Chloe E. Sokol, Joseph L. Galindo, Alexandra D. Bardas, Edward S. H. Koh, Justine Rozenich, Cassia Yeo, Maryanne Xu, et al.NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). We ligated adapters in the cDNA library using adapters and unique dual indexes from the NEBNext Multiplex

(20) Novel and improved Caenorhabditis briggsae gene models generated by community curationbioRxivMay 23, 2023Nicolas D. Moya, Lewis Stevens, Isabella R. Miller, Chloe E. Sokol, Joseph L. Galindo, Alexandra D. Bardas, Edward S. H. Koh, Justine Rozenich, Cassia Yeo, Maryanne Xu, et al.NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs, catalog no. E7775L). We ligated adapters in the cDNA library using adapters and unique dual indexes from the NEBNext Multiplex

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NEBNextUltraII RNA Library Prep with Sample Purification Beads

NEBNextUltraII RNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII RNA Library Prep with Sample Purification Beads from New England Biolabs

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