NEBNext Enzymatic Methyl-seq Conversion Module E7125S from New England Biolabs

Product Specs
ItemNEBNext Enzymatic Methyl-seq Conversion Module
CompanyNew England Biolabs
Price $185.00 Inquire
Catalog NumberE7125S
Quantity24 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Integrated targeted sequencing reveals unique tissue-of-origin and donor-derived cell-free DNA signatures in stable organ transplant recipientsiScienceMay 15, 2026Nicholas Kueng, Fanny Sandberg, Daniel Sidler, Vanessa Banz, Annalisa Berzigotti, Charlotte K.Y. Ng, Carlo R. Largiadèr, Ursula AmstutzUSA",Cat#103496 NEBNext Enzymatic Methyl-seq Conversion Module,"New England Biolabs, Ipswich, MA, USA",Cat#E7125L SRSLY NanoPlus kit,"Claret Bioscience, Santa Cruz, CA, USA",Cat#K155B-96 PhiX Control v3,"Illumina,

(2) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsieseLifeMay 1, 2026Spencer C Ding, Jingru Yu, Tiepeng Liao, Lauren S Ahmann, Yvette Y Yao, Chandler Ho, Linlin Wang, Benjamin A Pinsky, Wei Gucustom adapters, subjected to NEBNext Enzymatic Methyl-seq Conversion Module (New England Biolabs, #E7125L), and PCR-amplified. Sequencing and alignment Paired-end sequencing was performed on the NovaSeq X

(3) Adapting Clinical Chemistry Plasma as a Source for Liquid BiopsiesmedRxivFebruary 27, 2026Spencer C. Ding, Jingru Yu, Tiepeng Liao, Lauren S. Ahmann, Yvette Y. Yao, Chandler Ho, Linlin Wang, Benjamin A. Pinsky, Wei Gucustom adapters, subjected to NEBNext Enzymatic Methylseq Conversion Module (New England Biolabs, #E7125L), and PCR amplified. Sequencing and alignment Paired-end sequencing was performed on the NovaSeq X

(4) Tributyltin chloride alters the structural, genomic, and epigenomic integrity of postejaculatory mammalian spermEpigeneticsDecember 31, 2025Muhammad S. Siddique, Santosh Anand, Marie-Pierre L. Gauthier, Jason O. Brant, Michael P. Kladde, Ramji K. Bhandari, Bradford W. Daigneaultsperm After DNA isolation, Enzymatic Methyl-seq (EM-seq) Conversion kit module (New England Biolabs #E7125S) was used to convert gDNA cytosines to uridine followed by PCR amplification and downstream epigenomic

(5) 5‐Hydroxymethylcytosine Dynamics Reveals Coordinated Reprogramming of Parental Genomes and X Chromosome Dosage Balance in Mouse SCNT EmbryosAdvanced ScienceNovember 18, 2025Zeming Xiang, Rui Yan, Jing Guo, Mengyao Wang, Xin Cheng, Fan Zhang, Tianzi Guo, Xin Long, Fan Guo, Dan Liangat 55 °C for 10 min. Deamination was carried out using the APOBEC enzyme (New England Biolabs, Cat#E7125L) at 37 °C for 3 h. After deamination, the DNA was purified once more with AMPure XP beads before proceeding

(6) Comprehensive comparison of enzymatic and bisulfite DNA methylation analysis in clinically relevant samplesClin EpigeneticsOctober 3, 2025Barrett Nuttall, Daniel L. Karl, Kathleen Burke, Megan Callahan, Kerrin Mendler, Pablo Cingolani, Steven Criscione, Serhiy Naumenko, Elena Bibikova, Veerendra Munugalavadla, et al.external laboratory, an aliquot of each sample was processed with the enzymatic conversion module (E7125L, New England Biolabs), with up to 200 ng of input. In addition to the enzymatic-converted DNA, unconverted

(7) Improved circulating tumor DNA identification for detection of esophageal squamous cell carcinoma by enzymatic methyl sequencing and hybrid neural networkScientific ReportsSeptember 26, 2025Jiangfeng Shen, Yuqi Ren, Ziyong Mao, Qiuxiang Hu, Yilong Wan, Jiangcun Du, Yanting Lai, Shengxiang Shao, Liuqing Zhang, Hao Wu, et al.were subjected to the enzymatic conversion step using Enzymatic Methyl-seq Conversion Module (NEB, E7125S) according to the manufacturer’s protocol. The enzymatic converted DNA was then subjected to Accel-NGS

(8) Induction of a mismatch repair deficient genotype by tailored chemical mutagenesis in experimental models of cancerCancer CellJuly 14, 2025Benoit Rousseau, Mitesh Patel, Oliver Artz, Georgios Vlachos, Shrey Patel, Omar Hayatt, Guillem Argilés, Michael B. Foote, Lingqi Luo, Rachna Shah, et al.Qubit dsDNA HS Assay kit,Thermofischer scientific,Q32851 NEBNext Enzymatic Methyl-seq Conversion Module,New England Biolab,E7125L Methylated Mouse DNA Standard,Zymo,D5012 EpiTect MethyLight PCR + ROX Vial Kit,EpiTect,59496 D1000

(9) Cryogenic mouse tissue homogenization as an alternative to fresh-frozen biopsy use for genomics, transcriptomics, proteomics and metabolomicsSci RepJune 23, 2025Laimdota Zizmare, Ute Hofmann, Mohamed Ali Jarboui, Franziska Klose, Sabine Fraschka, Jakob Matthes, Marcel Krüger, Elke Schaeffeler, Matthias Schwab, Marius Ueffing, et al.(Cat. No 1000005251, kit version v2) with the NEBNext Enzymatic Methyl-seq Conversion Module (NEB #E7125S, version 3.1_2/20) and performed the library preparation according to the respective protocol except

(10) Methyl-CODEC enables simultaneous methylation and duplex sequencingNucleic Acids ResJune 4, 2025Ruolin Liu, Farzaneh Darbeheshti, Laurel Walsh, Rachel Li, Jin H Bae, Hayet Radia Zeggar, Azeet Narayan, Kan Xiong, G Mike Makrigiorgos, Viktor A Adalsteinssonmethyl-conversion step. Enzymatic methyl conversion NEBNext Methyl-seq Conversion Module (NEB, catalog no. E7125S) was used for DNA conversion based on the manufacturer’s protocol (Supplementary methods). Library amplification

(11) A DNA alteration and methylation co-detection method for clinical purposeEMBO Molecular MedicineJune 4, 2025Jiyan Yu, Chunhe Yang, Xintao Zhu, Zhankun Wang, Boping Xu, Ye Cai, Jingbo Zhao, Ruijian Guo, Wuzhou Yuan, Jianqing Wang, et al.Services Belmont Report. Preparation of spike-in control DNA Fully CpG-methylated pUC19 (0.1 ng/μl, NEB, E7125L) and fully unmethylated lambda DNA (2 ng/μl, NEB) were utilized as spike-in control DNA. A series of

(12) Methyl-Micro-C: simultaneous characterization of chromatin accessibility, interactions, and DNA methylationNAR Genom BioinformMay 27, 2025Leonardo Gonzalez-Smith, Claire Stevens, Huan Cao, Zexun Wu, Suhn K Rhiedeamination of unprotected unmethylated cytosines was performed, using EM-seq conversion module (NEB, E7125S). MMC libraries were generated by performing index PCR and sequenced using Illumina sequencing systems,

(13) ReQuant: improved base modification calling by k-mer value imputationNucleic Acids ResMay 10, 2025Roy Straver, Carlo Vermeulen, Joe R Verity-Legg, Marc Pagès-Gallego, Dieter G G Stoker, Alexander van Oudenaarden, Jeroen de Riddergenome Methylated genome was glucosylated using NEBNext Enzymatic Methyl-seq Conversion Module (NEB, E7125). 500 ng of genomic DNA (gDNA) in 15 μl was mixed with: 10 μl of reconstituted TET2 reaction buffer

(14) MSH2 is not required for either maintenance of DNA methylation or repeat contraction at the FMR1 locus in fragile X syndrome or the FXN locus in Friedreich's ataxiaEpigenetics ChromatinApril 28, 2025Jessalyn Grant-Bier, Kathryn Ruppert, Bruce Hayward, Karen Usdin, Daman Kumarienzymatically bisulfite converted using the NEBNext® Enzymatic Methyl-seq Conversion Kit (New England Biolabs, E7125S) which produced 100 µL of converted DNA at 2 ng/µL concentration. The bisulfite modified DNA was stored

(15) Programmable mRNA therapeutics for controlled epigenomic modulation of single and multiplexed gene expression in diverse diseasesNat CommunMarch 13, 2025Charles W. O'Donnell, Jeremiah D. Farelli, Houda Belaghzal, Justin Chen, Lauren Beech, James Sullivan, Chevaun Morrison-Smith, Stephen Siecinski, Adam Katz, Samuel Mildrum, et al.(Beckman-Coulter) and subjected to EM-conversion using the EM-seq Conversion Kit (New England Biolabs, Cat# E7125) according to the manufacturer’s instructions. Purified, converted DNA was PCR amplified for 40 cycles

(16) Integrating multi-omics features enables non-invasive early diagnosis and treatment response prediction of diffuse large B-cell lymphomaClin Transl MedJanuary 7, 2025Weilong Zhang, Bangquan Ye, Yang Song, Ping Yang, Wenzhe Si, Hairong Jing, Fan Yang, Dan Yuan, Zhihong Wu, Jiahao Lyu, et al.molecular identifier (UMI). For methylation conversion, NEBNEXT Enzymatic Methyl‐seq Conversion Module (Cat#E7125L, New England Biolabs) was used. The methylation library used the Qubit dsDNA HS Assay Kit (Cat#Q32854,

(17) The H3.3 K36M oncohistone disrupts the establishment of epigenetic memory through loss of DNA methylationMolecular CellOctober 17, 2024Joydeb Sinha, Jan F. Nickels, Abby R. Thurm, Connor H. Ludwig, Bella N. Archibald, Michaela M. Hinks, Jun Wan, Dong Fang, Lacramioara BintuM-280 Protein G,Thermo Fisher,Cat# 15575020 NEBNext Enzymatic Methyl-seq Conversion Module,NEB,Cat# E7125S CUTANA™ ChIC/CUT&RUN Kit,EpiCypher,Cat# 14–1048 SNAP-CUTANA™ K-MetStat Panel,EpiCypher,Cat# 19–1002 Cell Line Nucleofector Kit V

(18) Unraveling the key role of chromatin structure in cancer development through epigenetic landscape characterization of oral cancerMolecular cancerSeptember 6, 2024Xue Y, Liu L, Zhang Y, He Y, Wang J, Ma Z, Li TJ, Zhang J, Huang Y, Gao YQ.Methylation conversion was then conducted using NEBNext Enzymatic Methyl-seq Conversion Module (NEB, #E7125L) following the manufacturer’s instructions. Converted DNA libraries were amplified by NEBNext Q5U Master Mix

(19) Combinatorial quantification of 5mC and 5hmC at individual CpG dyads and the transcriptome in single cells reveals modulators of DNA methylation maintenance fidelityNature Structural & Molecular BiologyAugust 1, 2024Alex Chialastri, Saumya Sarkar, Elizabeth E. Schauer, Shyl Lamba, Siddharth S. Deyand subjected to nucleobase conversion using the NEBNext enzymatic methyl-seq conversion module (NEB, E7125S) according to the manufacturer’s recommendations except for performing the final elution step in 40

(20) Prostate cancer detection through unbiased capture of methylated cell-free DNAiScienceJuly 19, 2024Ermira Lleshi, Toby Milne-Clark, Henson Lee Yu, Henno W. Martin, Robert Hanson, Radoslaw Lach, Sabrina H. Rossi, Anja Lisa Riediger, Magdalena Görtz, Holger Sültmann, et al.Circulating Nucleic Acid Kit,Qiagen,Cat no. 55114 EM-seq Conversion module,New England Biolabs,Cat no. E7125L ACCEL-NGS Methyl-Seq DNA Library Kit,Swift,Cat no. 30024 SeqCap Epi Enrichment Kit,Roche NimbleGen,Cat

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NEBNext Enzymatic Methyl-seq Conversion Module

NEBNext Enzymatic Methyl-seq Conversion Module from New England Biolabs

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New England Biolabs

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Importance of Methylation Analysis and Application Areas

NEBNext Enzymatic Methyl-seq Conversion Module from New England Biolabs

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