Q5Site-Directed Mutagenesis Kit (Without Competent Cells) E0552S from New England Biolabs

Product Specs
ItemQ5Site-Directed Mutagenesis Kit (Without Competent Cells)
CompanyNew England Biolabs
Price $148.00 Inquire
Catalog NumberE0552S
Quantity10 reactions
ApplicationsDNA Manipulation
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) An optimized CRISPR/Cas9-based genome editing platform enhances bacteriocin production in Lactiplantibacillus plantarumJ Biol EngJuly 29, 2026Rajat Anand, Rudolf Lütticken, Laura De Laporte, Andreas Herrmann, Elisabeth Heinerespectively). Mutagenesis reactions were performed using a Q5® Site-Directed Mutagenesis Kit (NEB, Cat. no. E0552S). Sticky-end ligation was performed using the Instant Sticky-end Ligase Master Mix (NEB, Cat. no. M0370S).

(2) An Acetylation-Primed SUMOylation Switch Controls RORβ Stability through a p300-SIRT1 Regulatory AxisResearchSquareJuly 23, 2026Patrick Griffin, Timothy O'Leary, Denis Shutin, Nadeska Montalvan, Nereida Abad-Yang, Vuong Dang, Dean Edwards, Mi Ra Chang88836 Critical commercial assays,, NEB Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0552S RNeasy Mini Kit,Qiagen,Cat# 74104 High Capacity cDNA Reverse Transcription Kit,Applied Biosystems,Cat#

(3) Robust regulatory interplay of enhancers, facilitators, and promoters in a native chromatin contextCellJuly 17, 2026Zhou Zhou, Yang Cheng, Jaret Lieberth, Junke Zhang, Yiyang Jin, Albert Li, Haiyuan Yu, Abdullah Ozer, John T. Lis,Thermo Scientific,Cat#R0481 NEBuilder HiFi DNA Assembly Master Mix,NEB,Cat#E2621L Q5 site-directed mutagenesis kit,NEB,Cat#E0552S Q5 High-Fidelity 2X Master Mix,NEB,Cat#M0492L PrimeSTAR® GXL DNA Polymerase,TaKaRa,Cat#R050B Gateway

(4) Linking sugar sensing to immunity in plants through O-glycosylation of immune-signaling kinasesbioRxivJuly 8, 2026Yalikunjiang Aizezi, Hongliang Zhang, Hongliang Zhang, Jung-Gun Kim, Ajeet Chaudhary, Cao Son Trinh, Qingxi J. Shen, Shou-Ling Xu, Mary Beth Mudgett, Zhi-Yong Wang,New England Biolabs,E2611L PENTER/D-TOPO cloning kit,Thermo Scientific,450218 Q5 Site-Directed Mutagenesis Kit,New England Biolabs,E0552S Phusion DNA polymerase,Thermo Scientific,F530S Gateway LR Clonase II Enzyme mix,Thermo Scientific,11791020 Experimental models

(5) Acquisition of a novel restriction modification system regulates genetic flux and gene expression in the hypervirulent and globally disseminated CC17 lineage of group B StreptococcusNucleic Acids ResJuly 8, 2026Joana Alves, Chiara Crestani, Stephanie M Marroquin, Alix B E Johnston, Natalie Ring, Connor Bowen, Mark Reglinski, Kelly S Doran, Ruth N Zadoks, Nicola N Lynskeysite directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Ipswich, MA, USA, Catalog #E0552S) with primers GBSCOH1_0041_promC:A_SDM_1+2 (Table 2) to mutate nucleotide C to A at site −9 upstream

(6) VPS13C/PARK23 initiates lipid transfer and membrane remodeling for efficient lysosomal repairNat CommunJuly 2, 2026Oluwatobi Andrew Adeosun, Christian Schröer, Elisabeth Südhoff, Fabio Bergenthal, Katharina Sommer, Emely Döffinger, Britta Fiedler, Angelika Hilderink, Ann-Katrin Lehmann, Lea Sophie Pohle, et al.VPS13CΔVAB. Single amino acid substitutions were inserted by site-directed mutagenesis (New England Biolabs, E0552S). Respective primers for site-directed mutagenesis, Gibson assembly, and cloning were custom-synthesized

(7) Inherited human TFIIIA deficiency disrupts T cell developmentmedRxivJuly 1, 2026Evi Duthoo, Sueun Park, Tamara Jarayseh, Marita Bosticardo, Rafah Mackeh, Yana Van Droogenbroeck, Imke Velghe, Veronique Debacker, Hailun Li, Nourhen Agrebi, et al.Q5 Site-Directed Mutagenesis Kit, following the manufacturer’s instructions (New England Biolabs (NEB), E0552S). DNA concentration and purity were determined spectrophotometrically using the NanoDrop 2000 (Nanodrop Technologies

(8) Acetylation-Primed SUMOylation Drives RORβ Turnover via a p300-SIRT1 Regulatory AxisbioRxivJune 30, 2026Timothy R. O'Leary, Denis Shutin, Nadeska I. Montalvan, Nereida M. Abad-Yang, Vuong Dang, Dean P. Edwards, Patrick R. Griffin, Mi Ra Changcommercial assays,Critical commercial assays NEB Q5 Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0552S RNeasy Mini Kit,Qiagen,Cat# 74104 High Capacity cDNA Reverse Transcription Kit,Applied Biosystems,Cat#

(9) PHD1-dependent hydroxylation of RepoMan (CDCA2) on P604 modulates the control of mitotic progressionElifeJune 25, 2026Jimena Druker, Hao Jiang, Dilem Shakir, Fraser Child, Vanesa Alvarez, Melpomeni Platani, Andrea Corno, Constance Alabert, Adrian T Saurin, Jason R Swedlow, et al.This vector was used to generate pcDNA5-YFP-RepoManP604A using the site-directed mutagenesis kit (Q5#E0552S NEB), with the following primers: Forward 5’- TGAGATGACACGTTCCATTCCGAG-3’, Reverse 5’-GGGACTTCAGGCAGCTCG-3’

(10) Trametinib for multiple non-ossifying fibromas due to KRAS mosaic mutations: two case reportsCommunications MedicineMay 18, 2026Marie Vincent, Soizic Tiriau, Marine Fouillet-Desjonqueres, Alicia Besson, Wassim Ouchetto, Sébastien Barbarot, Bertrand Isidor, Aymeric Rouchaud, Geneviève Baujat, Pierre Vabres, et al.p.G13D or p.A146T variants were inserted using Q5 Site Directed Mutagenesis Kit (New England BioLabs, #E0552S) and the following primers: hsKRAS-G13D_F, 5’-GGAGCTGGTGaCGTAGGCAAG-3’, hsKRAS-G13D_R, 5’-AACTACCACAAGTTTATATTCAGTCATTTAC-3’,

(11) Noncanonical IRF3 function mediates STING-dependent pro-inflammatory cytokine production in macrophagesEMBO ReportsMay 8, 2026Katherine R Balka, Olivia R Lamb, Rajan Venkatraman, Zoe Magill, Le T Hoang, Ryker Dumbrill, Kate McArthur, Nicole A de Weerd, Paul J Hertzog, Peter J Crack, et al.740622 μ–Slide 8 well,Ibidi,Cat# 80826 Q5® Site-Directed Mutagenesis Kit,New England Biolabs,Cat# E0552S ChemiDoc™ Imaging System,Bio-Rad,Cat# 12003153 EVOS™ XL Core Imaging System,Thermo Fisher Scientific,Cat#

(12) Hid and reaper differentially regulate apoptotic and non-apoptotic executioner caspase activation to control organ sizeCellular and Molecular Life SciencesApril 27, 2026Min Hou, Xiaojiao Li, Yuanyuan Zhang, Huan Liu, Yuanlin Su, Huiyuan Ding, Yanqin Gong, Qiao Liu, Yaoqin Gong, Gongping Suncaspase-resistant L-CasExpressDQVA was made using Q5 Site-directed mutagenesis kit (New England Biolabs, Cat# E0552S) with the L-CasExpress plasmid [22] as the template. The plasmid was injected to attP2 site by UniHuaii

(13) Membranes arrest the coarsening of mitochondrial condensates in human cellsCommunications BiologyApril 23, 2026Sanjaya V. B. D. Aththawala Gedara, Surya Teja Penna, Marina FericNEBuilder HiFi DNA Assembly kit. ΔCRY2olig construct was made using a site-directed mutagenesis kit (NEB E0552S) with primers (forward primer: 5’-TAACGGCCGCGACTCTAGATCATAATCAGC-3’ and reverse primer: 5’-CTTGTACAGCTCGTCCATGCCGCC-3’).

(14) Inherited human CARD9 deficiency impairs lymphoid cell, but not fibroblast, IL-17–mediated immunityJCI InsightApril 22, 2026Erika Della Mina, Carlos G. El-Haddad, Timothy A. West, Clara W.T. Chung, Jing Jing Li, Vivienne Lea, Elissa K. Deenick, Filomeen Haerynck, Jean-Laurent Casanova, Anne Puel, et al.these plasmids by mutagenesis with appropriate primers, with the Q5 Site-Directed Mutagenesis kit (E0552S, New England Biolabs), according to the manufacturer’s protocol. Plasmids were amplified in competent

(15) Toxoplasma gondii effector GRA35 mediates neuronal damage via ER stress and mitochondria-associated apoptosisVirulenceApril 6, 2026Jie Wang, Ying Chen, Nan Zhou, Fangmin Li, Niuniu Dai, Zhiang Chen, Shutong Liu, Ran An, Lijian Chen, Jian Dudonated by Professor Shen Bang of Huazhong Agricultural University. The sentinel mutation kit(Catalog #: E0552S) was purchased from NEB. Multi-fragment one-step ligation kit(Catalog #: C113-01) was purchased from

(16) Sequence-dependent co-condensation of Lsr2 with DNA elucidates the mechanism of genome compaction in Mycobacterium tuberculosisNucleic Acids ResearchJanuary 8, 2026Prakshi Gaur, Thejas Satheesh, Rohit Kumar Singh, Hussain Beig, Sneha Shahu, Saminathan Ramakrishnan, Mansi Srivastava, Shreyasi Neogi, Ayesha Dash, Amit Singh, et al.N-terminus truncation (1–52) of Lsr2-Cys were prepared using Q5 site-directed mutagenesis kit (NEB E0552S). All the clones were confirmed by sequencing. The primer sets used for these mutants are listed in

(17) RNA-coupled CRISPR screens reveal ZNF207 as a regulator of LMNA aberrant splicing in progeriaMolecular CellJanuary 8, 2026Amit K. Behera, Jeongjin J. Kim, Shreya Kordale, Filip Pekovic, Arun Prasath Damodaran, Bandana Kumari, Sandra Vidak, Ethan Dickson, Mei-Sheng Xiao, Gerard Duncan, et al.construct was then modified by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis Kit (NEB #E0552S) to introduce silent mutations for resistance to the most effective ZNF207-targeting siRNA (#1: CAACTAGTGCAACCAGTAA). Next,

(18) EGR Proteins Mediate Interferon‐Independent Anti‐HSV‐1 Responses Through Viral and Host TargetsAdvanced ScienceJanuary 4, 2026Shuaishuai Wang, Fujun Hou, Xunuo Jin, Yuhang Xiang, Dongli PanpLVX‐EGR1. Most mutations were introduced into pLVX‐EGR1 using a Q5 Site‐Directed Mutagenesis Kit (NEB, #E0552S). For example, using pLVX‐EGR1 as the template, PCR was performed using EGR1Δ(AD‐N)‐F and EGR1Δ(AD‐N)‐R

(19) Functional analysis of AIP variants in a cohort of neuroendocrine neoplasmsEndocrine-Related CancerJanuary 1, 2026Blanca R Carranza-Zavala, Julia M Zuarth-Vázquez, Susana M López-Zelocualtecatl, Claudia Ramírez-Rentería, Maribel Rodríguez-Torres, Ernesto Sosa-Eroza, Baldomero González-Virla, Armando Gamboa-Domínguez, Alfredo A Reza-Albarrán, Laura C Hernández-Ramírezgenerate four variants, using either the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA, E0552S) or the QuikChange II XL Kit (Agilent, USA, 200521) with the following primers: 5′-CACCGCGATGTTGCAGAGACTCTTGGCCA-3′

(20) Functional analysis of AIP variants in a cohort of neuroendocrine neoplasmsEndocr Relat CancerDecember 19, 2025Blanca R Carranza-Zavala, Julia M Zuarth-Vázquez, Susana M López-Zelocualtecatl, Claudia Ramírez-Rentería, Maribel Rodríguez-Torres, Ernesto Sosa-Eroza, Baldomero González-Virla, Armando Gamboa-Domínguez, Alfredo A Reza-Albarrán, Laura C Hernández-Ramírezgenerate four variants, using either the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, USA, E0552S) or the QuikChange II XL Kit (Agilent, USA, 200521) with the following primers: 5′-CACCGCGATGTTGCAGAGACTCTTGGCCA-3′

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10/28/2021Great Site-Directed Mutagenesis Kit Wei WeiThis is great site-directed mutagenesis kit that introduces mutations at specifc locations in plasmids.

Q5Site-Directed Mutagenesis Kit (Without Competent Cells)

Q5Site-Directed Mutagenesis Kit (Without Competent Cells) from New England Biolabs

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New England Biolabs

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Q5Site-Directed Mutagenesis Kit (Without Competent Cells) from New England Biolabs

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