NEBNext Ultra IIEnd Repair/dA-Tailing Module E7546S from New England Biolabs

Product Specs
ItemNEBNext Ultra IIEnd Repair/dA-Tailing Module
CompanyNew England Biolabs
Price $255.00 Inquire
Catalog NumberE7546S
Quantity24 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Technical feasibility of a long read, fourth generation sequencing platform in diagnostic profiling of clinical routine samples: a proof-of-concept studyPathologicaMay 31, 2026Domenico Vitale, Lorenzo Colarossi, Gianluca Russo, Claudia Scimone, Domenico Cozzolino, Caterina De Luca, Andrey D. Prjibelski, Alla Mikheenko, Cristina Colarossi, Francesco Delli Muti, et al.(Genedin) was end-repaired and adapter ligated using the NEBNext Ultra II End Repair/dA-Tailing Kit (E7546S, NEB). Briefly, 1.75 μl of ULTRA II end prep reaction buffer and 0.75 μl of ULTRA II end prep enzyme

(2) Improved Cas9-targeted nanopore sequencing facilitates ultra-deep analysis of genomic variationCell Reports MethodsMay 18, 2026Nick Vereecke, Megan Behrmann, Anupama Khare, John P. DekkerMaster Mix (2X),NEB,M0323S AMPure XP beads,Beckman Coulter,A63881 EB Ultra-II End-prep Enzyme Mix,NEB,E7546S Long-Fragment Buffer (LFB),ONT,Part of SQK-LSK114 Brain Heart Infusion Broth,Sigma,53286 Yeast extract,Oxoid,LP0021 Porcine

(3) Genetic modification of intractable bacterial clones by heat shock-facilitated phage transductionCell Reports MethodsMay 18, 2026Lukas Schulze, Jens Stahl, Nastassia J. Knoedlseder, Sophia Krauss, Theresa Harbig, Kay Nieselt, Holger Brueggemann, Bernhard Krismer, Andreas Peschelwere supplemented with the necessary reagents from the NEBNext Ultra II end repair/dA tailing kit (E7546S; New England Biolabs [NEB]) and the reaction was incubated at 20°C for 5 min and then at 65°C for 5

(4) Nested mobile genetic elements mediating antimicrobial resistance genes mobility within and between Acinetobacter isolatesiScienceMay 15, 2026Kenneth Bongulto, Ngure Kagia, Hisamichi Tauchi, Satoru Suzuki, Kozo WatanabeMix,New England BioLabs,M6630S NEBNext® Ultra II End repair/dA-tailing Module,New England BioLabs,E7546S NEBNext® Quick Ligation Module,New England BioLabs,E6056S Oxford Nanopore Technologies Ligation Sequencing

(5) Phage homing endonuclease amplifies anti-defense genes to evade bacterial immunityNat CommunApril 20, 2026Kotaro Chihara, Aa Haeruman Azam, Artyom A. Egorov, Ilya Terenin, Masanori Hashino, Koichi Watashi, Kazuhiro Horiba, Vasili Hauryliuk, Kotaro Kigarepair and dA-tailing were performed using the NEBNext Ultra II End Repair/dA-Tailing Module (NEB, #E7546S) according to the manufacturer’s specifications. Adapter 1 oligos were annealed in 1× annealing buffer

(6) Phage-assisted evolution of allosteric protein switchesNat CommunApril 14, 2026Nicholas T. Southern, Anna von Bachmann, Alisa Hovsepyan, Marielouise Griebl, Benedict Wolf, Nina Lemmen, Ann-Sophie Kroell, Simon Westermann, Jan Mathony, Dominik Niopekwere performed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, cat. no. E7546S), followed by barcode adapter ligation with the NEB Blunt/TA Ligase Master Mix (New England Biolabs,

(7) Essentialome-Wide Multigenerational Imaging Reveals Mechanistic Origins of Cell Growth LawsbioRxivApril 12, 2026Daniel S. Eaton, Carlos Sánchez, Luis Gutiérrez-López, Jacob Q. Shenker, Youlian Goulev, Brianna R. Watson, Véronique Henriot, Ethan C. Garner, Quincey A. Justman, Jeffrey R. Moffitt, et al.Nanopore Technologies (ONT) which included FFPE DNA Repair (NEB, M6630S), End Repair/dA-Tailing (NEB, E7546S), and ligation of adaptors from the ONT SQK-LSK109 ligation sequencing kit. Prepared amplicons were

(8) NPM1 mislocalization mediated by RNA Pol I inhibition alters chromatin landscapebioRxivFebruary 23, 2026Pierre-Olivier Estève, Sagnik Sen, Karthikeyan Raman, Ashwin Unnikrishnan, Sriharsa Pradhan−20°C until the next day. DNA repair/dA tailing was performed (UltraII protocol for Illumina; NEB # E7546S). Illumina adapters were diluted 1/5 for input; 1/25 for IP samples and ligated for 2hr at RT. USER

(9) Antibody escape drives emergence of diverse spike haplotypes resembling variants of concern in persistent SARS-CoV-2 infectionsCell Reports MedicineFebruary 17, 2026Luke B. Snell, Suzanne Pickering, Adela Alcolea-Medina, Helena Winstone, Jeffrey Seow, Carl Graham, Lorcan O’Connell, Rahul Batra, Michael H. Malim, Katie J. Doores, et al.,Oxford Nanopore Technologies,Cat #GRD-MK1 NEBNext® Ultra™ II End Repair/dA-Tailing Module,New England Biolabs,Cat #E7546S NEBNext® Quick Ligation Module,New England Biolabs,Cat#E6056S SARS-CoV-2 N gene standard,TIB MOLBIOL,Cat#30-7454-71 Luna®

(10) Temporal dynamics of collateral RNA cleavage by LbuCas13a in human cellsCommunications BiologyJanuary 19, 2026Jorik Frederik Bot, Zhihan Zhao, Mengyuan Li, Darnell Kammeron, Peng Shang, Niels GeijsenA63882) at a ratio of 2 beads to 1 sample. The NEBNext Ultra II End Repair/dA-tailing Module (NEB, E7546S) was used before ligating Nanopore native barcodes (EXP-NBD104&114). Finally, samples were pooled at

(11) Expression of ultralong complementarity determining region 3 and development of IgM and IgG B cell receptor repertoires in Holstein heifer calvesImmunoHorizonsJanuary 14, 2026Tess E Altvater-Hughes, Harold P Hodgins, Douglas C Hodgins, Cathy A Bauman, Bonnie A MallardNEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Ipswich, Massachusetts, USA, catalog no. E7546S). Samples were washed and eluted in nuclease-free water. Samples were then barcoded and prepared using

(12) Sarcomeric remodelling in human heart failure unraveled by single molecule long read sequencingEMBO Molecular MedicineJanuary 13, 2026Jan Haas, Sarah Schudy, Benedikt Rauscher, Ana Muñoz Verdú, Steffen Roßkopf, Christoph Reich, Gizem Donmez Yalcin, Abdullah Yalcin, Timon Seeger, Christoph Dieterich, et al.obtain 5’phosphorylated and 3’dA-tailed sites was performed with Ultra II End-prep module from NEB (#E7546S), and DNA was purified with 60 μL AMPure XP beads. The provided nanopore ONT adapters were ligated,

(13) Systematic enhancer mapping and functional analysis in zebrafish with optimized CRISPR interferenceNucleic Acids ResDecember 11, 2025Jiulin Chan, Zhichao Wu, Mingli Liu, Tianming Wang, Hanyu Liu, Ruimeng Cao, Xiaolong Li, Xinwen Li, Siyao Zhan, Jiangbo Cheng, et al.adaptor ligation were performed with the NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB, Cat#E7546S). End repair was performed at 20°C for 30 min and at 65°C for 30 min, followed by adaptor ligation at

(14) Base editing strategies for in vivo correction of two highly recurrent phenylketonuria variantsMolecular Therapy Nucleic AcidsDecember 9, 2025Aidan Quigley, Ishaan Jindal, Thomas Campion, Delaney Rutherford, Yongseok Han, Walsh Quigley, Ping Qu, Kiran Musunuru, Rebecca Ahrens-Nicklas, Xinying Hong, et al.min, Klenow fragment (NEB #5288) at 37°C for 30 min, and NEBNext Ultra II End Prep Enzyme Mix (NEB #E7546S) at 20°C for 30 min followed by 65°C for 30 min, with 2× AMPure XP bead (Beckman #A63881) purification

(15) CLAE: A High‐Fidelity Nanopore Sequencing Strategy for Read‐Level Viral Variant Detection and Environmental RNA Virus DiscoveryAdvanced ScienceNovember 1, 2025Hannah Yu, Sarah Golconda, Ga‐Eun Lee, Dantong Xue, Guillermo Domínguez‐Huerta, James M. Wainaina, Benjamin Bolduc, Shashanka Murthy, Shihyoung Kim, Seth Faith, et al.end‐repaired and A‐tailed using NEBNext FFPE Repair (NEB M6630) and NEBNext Ultra II End Prep kit (NEB E7546S) according to the manufacturer's instructions. RCA‐Hairpin Ligation to A‐Tailed Double‐Stranded DNA To

(16) SCoTCH-seq reveals that 5-hydroxymethylcytosine encodes regulatory information across DNA strandsProc Natl Acad Sci U S AAugust 5, 2025Jack S. Hardwick, Somdutta Dhir, Angie Kirchner, Angela Simeone, Sean M. Flynn, James M. Edgerton, Rafael de Cesaris Araujo Tavares, Isabel Esain-Garcia, David Tannahill, Paula Golder, et al.A8569). Samples were end-repaired and A-tailed (NEB Ultra II End Repair/dA-Tailing Module, catalog number E7546S) and adapter-ligated (NEB Ultra II Ligation Module, catalog number E7595S) according to the manufacturer’s

(17) Optogenetic Clustering of Human IRE1 Reveals Differential Regulation of Transcription and mRNA Splice Isoform Abundance by the UPRbioRxivJuly 24, 2025Jacob W. Smith, Damien B. Wilburn, Vladislav Belyyenzyme and 7 μl of buffer from the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs E7546S) were added to the DNA pool and incubated at 20°C for 5 min and 65°C for 5 min. End-repaired DNA was

(18) Aeromonas spp. as a fast-growing high-performance chassis for protein productionApplied and Environmental MicrobiologyJuly 23, 2025Ming-Xuan Tang, Peng-Fei Meng, Ruo-Lin Huang, Xin Zheng, Chen-Chen Liang, Xuepiao Pu, Chen Wang, Ying Zhao, Yi-Qiu Zhang, Jia-Xin Liang, et al.end-repair and dA-tailing using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, #E7546S). The DNA was then cleaned with AMPure XP beads (Beckman Coulter, #A63882) and eluted in 31 µL of nuclease-free

(19) Integrated Taxonomy Discovers Four New Species of Grypoctonus Speiser, 1928 (Diptera: Asilidae) from China †InsectsJuly 15, 2025Haoyue Zhou, Ding Yang, Xuankun Liperformed using the SQK-LSK114 Ligation Sequencing Kit (Oxford Nanopore Technologies, Oxford, England), NEB#E7546S Ultra II End Repair Ligation Module (New England Biolabs Ltd., Beijing, China), and NEB#E6056S Quick

(20) Methyl-Micro-C: simultaneous characterization of chromatin accessibility, interactions, and DNA methylationNAR Genom BioinformMay 27, 2025Leonardo Gonzalez-Smith, Claire Stevens, Huan Cao, Zexun Wu, Suhn K Rhiebiotinylated bridge sequence. Two hundred nanograms of ligated DNA products underwent end preparation (NEB, E7546S), and methylated adapters (NEB, E7165AAVIAL) were added. After enriching ligated products, oxidation

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NEBNext Ultra IIEnd Repair/dA-Tailing Module

NEBNext Ultra IIEnd Repair/dA-Tailing Module from New England Biolabs

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New England Biolabs

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NEBNext Ultra IIEnd Repair/dA-Tailing Module from New England Biolabs

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