NEBNext Ultra IIEnd Repair/dA-Tailing Module E7546L from New England Biolabs

Product Specs
ItemNEBNext Ultra IIEnd Repair/dA-Tailing Module
CompanyNew England Biolabs
Price $811.00 Inquire
Catalog NumberE7546L
Quantity96 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

Add to Compare List

New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Arrayed dual-gRNA CRISPR Screening Platform for C9orf72 Repeat Expansion Excision in Patient iPSCsMolecular Therapy AdvancesJune 11, 2026Olubankole Aladesuyi Arogundade, Katie Jing Kay Kay Lam, Katherine A. Brown, Tanya Jain, Patrick O. Issagholian-Lewin, Cerianne Huang, Taylor Rae-Hudson, Kevin Briseno, Stacia K. Wyman, Netravathi Krishnappa, et al.Tapestation, Agilent]). Fragmented DNA was end-repaired (NEBNext UltraII End Repair/dA-Tailing Module, NEB E7546L; 30 min 20°C, 30 min 65°C) before a custom P7 adapter36 was ligated (NEBNext Ultra II Ligation Module,

(2) Enriched methylomes of low-input and fragmented DNA using fragment ligation EXclusive methylation sequencingNucleic Acids ResMay 14, 2026Jingru Yu, Lauren S Ahmann, Yvette Y Yao, Angus Toland, Alicia Snowden, Chandler Ho, Netanel Loyfer, Tommy Kaplan, Hannes Vogel, Linlin Wang, et al.input amounts. End repair and adaptor ligation were performed using the NEBNext End Repair/dA-Tailing (E7546L, NEB) and Ligation (E7595L, NEB) module. All reagent volumes were halved according to the manufacturer’s

(3) Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progressionNeoplasiaMarch 1, 2026Shams Al-Azzam, Isabella Stuewe, Sunandini Sharma, Miki Yamada-Hara, Arisachi Tanaka, Kegan Stringer, Merna Behnam, Norah Al-Azzam, Shuvro Nandi, Maria Zhivagui, et al.v2 MC Kit,Roche,Cat#10010206 NEBNext Ultra II End Repair/dA-Tailing Module,New England Biolabs,Cat# E7546L NEBNext Ultra II Ligation Module,New England Biolabs,Cat# E7595L Infinium Mouse Methylation BeadChip

(4) Sensitive, flexible, and affordable serum RNA sequencing for pathogen detection on the Oxford Nanopore platformBMC GenomicsFebruary 12, 2026Philippe Selhorst, Emilie Van Vyve, Francesca Falconi-Agapito, Joachim Mariën, Kevin K. Ariënand sequencing The amplified DNA was end-prepped using the Ultra II End Repair/dA-Tailing Module (E7546L, NEB) by pooling 6.4 ng of each of four samples added up to a maximum of 40 µL with water. 10 µL end-prep

(5) Unraveling the intra-species genomic diversity of sweetpotato-infecting CRESS-DNA and RNA viruses in Burkina Faso using Oxford Nanopore sequencingFrontiers in MicrobiologyFebruary 4, 2026Pakyendou E. Name, Ezechiel B. Tibiri, Fidèle Tiendrébéogo, Seydou Sawadogo, Florencia Djigma, Lassina Traoré, Angela O. Eni, Justin S. Pitatreated using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, MA, USA, Cat. No. E7546L), with an incubation period of 5 min at 20 °C, followed by 5 min at 65 °C. Following the purification

(6) Impact of bovine lactoferrin supplementation and reduced iron in formula on infant oral microbiome: a randomized controlled trialJournal of Oral MicrobiologyDecember 31, 2025Cynthia Anticona, Anders Esberg, Staffan K. Berglund, Maria Björmsjö, Olle Hernell, Bo Lönnerdal, Ingegerd Johansson, Pernilla Lif Holgerson200 fmol PCR products were end-repaired using NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB, E7546L) of which 10 fmol was used for ligation to unique barcodes for each sample using NEB Blunt/TA Ligase Master Mix

(7) The circulating cell-free DNA landscape in sepsis is dominated by impaired liver clearanceCell GenomicsOctober 8, 2025Kiki Cano-Gamez, Patrick Maclean, Masato Inoue, Sakineh Hussainy, Elisabeth Foss, Chloe Wainwright, Hanyu Qin, Stuart McKechnie, Chun-Xiao Song, Julian C. Knight,New England Biolabs,Cat# M0531S NEBNext Ultra™ II end-repair/dA-tailing module,New England Biolabs,Cat# E7546L KAPA HyperPrep kit,Roche,Cat# 07962363001 KAPA HiFi HotStart Uracil+ ReadyMix kit,Roche,Cat# 07959079001 AMPure

(8) Genomic insights into diversity, antimicrobial resistance and virulence of Glaesserella parasuis from diseased swine in PeruFrontiers in MicrobiologySeptember 23, 2025Luis Alvarez-Vega, Karolina S. M. De La Cruz-Monroy, Arturo Garcia-Barraza, Dennis Carhuaricra, Jose L. Llaja-Bernedo, Liseth L. Huamancha-Pulido, Alejandra Medina-Gonzales, Andre F. Sedano-Sanchez(M6630L, New England Biolabs, USA) and A-tails were added using the Ultra II End Repair/dA-Tailing Module (E7546L, New England Biolabs, USA). Native barcodes (SQK-NBD114.24, Oxford Nanopore, UK) were ligated to DNA

(9) The Diversity of the DNA ‐Binding Landscape in the DREB / ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous GenomePlant Biotechnology JournalSeptember 18, 2025Fengqi Wu, Jiakun Zheng, Huimin Hu, Hongsen Liu, Yaxuan Xiao, Junting Feng, Yanwei Hao, Chengjie Chen, Rui Xia, Zaohai Zengfragments were performed using the NEBNext Ultra II End Repair/dA‐Tailing and Ligation Module (NEB, E7546L, E7595L). The coding sequences of LcERFs were amplified by PCR using cDNA from ‘FZX’ as the template,

(10) Insights into Novel Viral Threats in Sweetpotato from Burkina Faso: Characterisation of Unexplored PathogensVirusesSeptember 7, 2025Pakyendou E. Name, Ezechiel B. Tibiri, Fidèle Tiendrébéogo, Seydou Sawadogo, Florencia Djigma, Lassina Traoré, Angela O. Eni, Justin S. PitadA-tailed using the NEBNext Ultra II End repair/dA-tailing module (New England Biolabs, MA, USA, Cat. No. E7546L). After incubation at 20 °C for 5 min and 65 °C for 5 min, DNA was purified and the native barcodes

(11) Multi-analyte approach combining cfDNA sequencing and protein testing for early ovarian cancer detectioniScienceJune 20, 2025Fenfen Wang, Lingfang Wang, Ziyu Xing, Lingjia Lu, Zhuoqun Lin, Senmi Qian, Tao Zhu, Zhuyan Shao, Lingjun Zhao, Jie Dong, et al.high-throughput sequencing reagent set,MGI,Cat#1000013853 NEBNext Ultra II End Repair/dA-Tailing Module,NEB,Cat#E7546L NEBNext Ultra II Ligation Module,NEB,Cat#E7595L KAPA Hyper Prep Kit,KAPA Biosystems,Cat#KK8504 MGISEQ-2000RS

(12) SIRT7 regulates NUCKS1 chromatin binding to elicit metabolic and inflammatory gene expression in senescence and liver agingMolecular CellJune 19, 2025Khoa A. Tran, Michael Gilbert, Berta N. Vazquez, Alessandro Ianni, Benjamin A. Garcia, Alejandro Vaquero, Shelley L. BergerNEBNext Poly(A) mRNA Magnetic Isolation Module kit (NEB Cat # 7490L) followed by NEBNext Ultra II Direction Prep Kit for Illumina (NEB Cat # E7546L). Library sizes was measured using Bioanalyzer and pooled libraries concentration was calculated using

(13) Rifampin mediated decapsulation and enhanced bacterial clearance: a new mechanism of rifamycinsEBioMedicineJune 4, 2025Gi Young Lee, Jeongmin SongMaxima H Minus Double-Stranded cDNA Synthesis Kit (ThermoFisher, cat# K2561). The purified dscDNA library was end-repaired (NEB, #cat E7546L) and barcoded (ONT, #cat EXP-NBD104). All the barcoded samples were pooled for adaptor ligation (AMII,

(14) Clinico-genomic study reveals association of dengue virus genome high frequency mutations with dengue disease severitySci RepMay 28, 2025Varsha Ravi, Md Imran, Kriti Khare, Pallavi Mishra, Ramakant Mohite, Kanika, Md Abuzar Khan, Aparna Swaminathan, Aanchal Yadav, Sristi Sinha, et al.underwent end repair using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Cat. No. E7546L). Unique barcodes were then ligated to the end-prepped PCR products using the Rapid Barcode Kit (SQK-RBK110-96)

(15) The fire ant social chromosome exerts a major influence on genome regulationMol Biol EvolMay 24, 2025Beryl M Jones, Alex H Waugh, Michael A Catto, Sasha Kay, Karl M Glastad, Michael A D Goodisman, Sarah D Kocher, Brendan G Huntadapters were ligated to size-selected genomic DNA using the NEBNext Ultra II End Repair Module (NEB E7546L) with 1 µg fragmented DNA per genotype. Adapter-ligated DNA libraries were then cleaned with a 1.8×

(16) Inter-host diversity associated with age, sex, and menstrual cycle modulates clinical manifestations in DENV-2 patientsiScienceMay 16, 2025Kriti Khare, Md Imran, Varsha Ravi, Ramakant Mohite, Sayanti Halder, Pallavi Mishra, Bansidhar Tarai, Sandeep Budhiraja, Tavpritesh Sethi, Rajesh Pandeyand A-tailing using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Cat. No. E7546L). Barcoding was performed with the Native Barcoding Kit 24v14 (SQK-NBD114.24), and barcoded libraries

(17) Spatiotemporal 3D chromatin organization across multiple brain regions during human fetal developmentCell DiscovMay 16, 2025Yaoyu Sun, Min Li, Chao Ning, Lei Gao, Zhenbo Liu, Suijuan Zhong, Junjie Lv, Yuwen Ke, Xinxin Wang, Qiang Ma, et al.Then the beads binding with fragmented DNA were treated with the End Repair/dA-Tailing Module (NEB, E7546L) and Ligation Module (NEB, E7595L) following the operation manual. After ligation, the beads were washed

(18) A telomere‐to‐telomere gap‐free assembly integrating multi‐omics uncovers the genetic mechanism of fruit quality and important agronomic trait associations in pomegranatePlant Biotechnology JournalMay 3, 2025Lina Chen, Hao Wang, Tingtao Xu, Ruitao Liu, Juanli Zhu, Haoxian Li, Huawei Zhang, Liying Tang, Dan Jing, Xuanwen Yang, et al.High‐molecular‐weight genomic DNA was purified by magnetic beads, after damage and end repair (NEB, M6630L and E7546L), sequencing adapters ligation (NEB, E6056S), ONT ultralong sequencing libraries were prepared with

(19) Genomic hotspots in the DENV-2 serotype (E, NS4B, and NS5 genes) are associated with dengue disease severity in the endemic region of IndiaPLoS Negl Trop DisApril 29, 2025Varsha Ravi, Kriti Khare, Ramakant Mohite, Pallavi Mishra, Sayanti Halder, Richa Shukla, Chinky Shiu Chen Liu, Aanchal Yadav, Jyoti Soni, Kanika, et al.were performed using the NEBNext Ultra II End Repair/dA-Tailing Module (New England Biolabs, Cat. No. E7546L), followed by barcode ligation (Native Barcoding Kit 24v14; SQK-NBD114.24). The barcoded library was

(20) Tissue origin of endothelial cells determines immune system modulation and regulation of HIF-1α-, TGF-β-, and VEGF signalingiScienceFebruary 21, 2025Robin Heiden, Laura Hannig, Jakob S. Bernhard, Mario Vallon, Anja Schlecht, Nico Hofmann, Süleyman Ergün, Franziska Hoschek, Maximilian Wagner, Andreas Neueder, et al.beads and taken forward to end preparation with the NEBNext Ultra II End Repair/dA-Tailing Module (#E7546L, NEB). After bead cleanup, native barcode ligation was performed. Each sample was labeled with a single

Be the First to Write a Review!

NEBNext Ultra IIEnd Repair/dA-Tailing Module

NEBNext Ultra IIEnd Repair/dA-Tailing Module from New England Biolabs

Description

New England Biolabs

Citations (57)

Reviews

NEBNext Ultra IIEnd Repair/dA-Tailing Module from New England Biolabs

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map