NEBNext Ultra II Ligation Module E7595L from New England Biolabs

Product Specs
ItemNEBNext Ultra II Ligation Module
CompanyNew England Biolabs
Price $1,295.00 Inquire
Catalog NumberE7595L
Quantity96 reactions
ApplicationsDNA Analysis, Ligation, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multi-omics profiling reveals microenvironmental remodeling as a key driver of house dust mite-induced lung cancer progressionNeoplasiaMarch 1, 2026Shams Al-Azzam, Isabella Stuewe, Sunandini Sharma, Miki Yamada-Hara, Arisachi Tanaka, Kegan Stringer, Merna Behnam, Norah Al-Azzam, Shuvro Nandi, Maria Zhivagui, et al.NEBNext Ultra II End Repair/dA-Tailing Module,New England Biolabs,Cat# E7546L NEBNext Ultra II Ligation Module,New England Biolabs,Cat# E7595L Infinium Mouse Methylation BeadChip Kit v1.0,Illumina,Cat# 20041558 Deposited data,Deposited data,Deposited

(2) Susceptibility and resistance of Gram-negative bacteria to a novel antimicrobial agent TGV-49Frontiers in AntibioticsNovember 3, 2025Victor V. Tetz, Kristina M. Kardava, Maria F. Vecherkovskaya, Semen A. Leyn, Marinela L. Elane, Andrei L. Osterman, George V. Tetzsequencing were prepared using a NEBNext Ultra II FS DNA library prep kit for Illumina modules E7810L and E7595L (New England BioLabs) as per the manufacturer’s protocol (DNA input ≥100 ng); TruSeq DNA PCR-free CD

(3) The Diversity of the DNA ‐Binding Landscape in the DREB / ERF Family: Focusing on Reproductive Processes in Fruit Trees With Highly Heterozygous GenomePlant Biotechnology JournalSeptember 18, 2025Fengqi Wu, Jiakun Zheng, Huimin Hu, Hongsen Liu, Yaxuan Xiao, Junting Feng, Yanwei Hao, Chengjie Chen, Rui Xia, Zaohai Zengfragments were performed using the NEBNext Ultra II End Repair/dA‐Tailing and Ligation Module (NEB, E7546L, E7595L). The coding sequences of LcERFs were amplified by PCR using cDNA from ‘FZX’ as the template, with the

(4) Multi-analyte approach combining cfDNA sequencing and protein testing for early ovarian cancer detectioniScienceJune 20, 2025Fenfen Wang, Lingfang Wang, Ziyu Xing, Lingjia Lu, Zhuoqun Lin, Senmi Qian, Tao Zhu, Zhuyan Shao, Lingjun Zhao, Jie Dong, et al.set,MGI,Cat#1000013853 NEBNext Ultra II End Repair/dA-Tailing Module,NEB,Cat#E7546L NEBNext Ultra II Ligation Module,NEB,Cat#E7595L KAPA Hyper Prep Kit,KAPA Biosystems,Cat#KK8504 MGISEQ-2000RS High-Throughput Sequencing Reagent Kit

(5) Spatiotemporal 3D chromatin organization across multiple brain regions during human fetal developmentCell DiscovMay 16, 2025Yaoyu Sun, Min Li, Chao Ning, Lei Gao, Zhenbo Liu, Suijuan Zhong, Junjie Lv, Yuwen Ke, Xinxin Wang, Qiang Ma, et al.fragmented DNA were treated with the End Repair/dA-Tailing Module (NEB, E7546L) and Ligation Module (NEB, E7595L) following the operation manual. After ligation, the beads were washed with 1× TWB twice and 1× Tris

(6) Cancer-independent somatic mutation of the wild-type NF1 allele in normal tissues in neurofibromatosis type 1Nature GeneticsFebruary 25, 2025Thomas R. W. Oliver, Andrew R. J. Lawson, Henry Lee-Six, Anna Tollit, Hyunchul Jung, Yvette Hooks, Rashesh Sanghvi, Matthew D. Young, Timothy M. Butler, Pantelis A. Nicola, et al.into the ligation reaction mix, which consisted of 30 μl Ultra II Ligation MM (New England Biolabs, E7595L), 1 μl Ultra II ligation enhancer (New England Biolabs, E7595L), 1.25 μl xGen Duplex Seq Adapters (Integrated

(7) Barcoded HIV-1 reveals viral persistence driven by clonal proliferation and distinct epigenetic patternsNat CommunFebruary 14, 2025Tian-Hao Zhang, Yuan Shi, Natalia L. Komarova, Dominik Wordaz, Matthew Kostelny, Alexander Gonzales, Izra Abbaali, Hongying Chen, Gabrielle Bresson-Tan, Melanie Dimapasoc, et al.Repair/dA-Tailing Module (New England Biolabs, E7546L) and NebNext UltraII Ligation Module (New England Biolabs, E7595L). A six-nucleotide multiplexing ID was used to distinguish among different samples. Deep sequencing

(8) Ultra-sensitive molecular residual disease detection through whole genome sequencing with single-read error correctionEMBO Molecular MedicineAugust 20, 2024Xinxing Li, Tao Liu, Antonella Bacchiocchi, Mengxing Li, Wen Cheng, Tobias Wittkop, Fernando L Mendez, Yingyu Wang, Paul Tang, Qianqian Yao, et al.ultrasonicator, followed by library construction using NEBNext® Ultra™ II Ligation Module (catalog no. E7595L, NEB) and NEBNext® Ultra™ II End Repair/dA-Tailing Module (catalog no. E7546L, NEB). The cfDNA libraries

(9) Two classes of DNA gyrase inhibitors elicit distinct evolutionary trajectories toward resistance in gram-negative pathogensnpj Antimicrobials and ResistanceMarch 2, 2024Semen A. Leyn, James E. Kent, Jaime E. Zlamal, Marinela L. Elane, Maarten Vercruysse, Andrei L. Ostermansequencing were prepared using a NEBNext Ultra II FS DNA library prep kit for Illumina modules E7810L and E7595L (New England BioLabs) by the manufacturer’s protocol (DNA input ≥100 ng) but using TruSeq DNA PCR-free

(10) Identification of plasmids in avian-associated Escherichia coli using nanopore and illumina sequencingBMC GenomicsNovember 21, 2023Haley Sanderson, Madeline C. McCarthy, Chinenye R. Nnajide, Jessica Sparrow, Joseph E. Rubin, Jo-Anne R. Dillon, Aaron P. Whiteat 65 °C. NEBNext Adaptors were ligated to the fragmented DNA using NEBNext Ultra II Ligation Mix (#E7595L; NEB) by incubating at 20ºC for 15 min. To purify the DNA and remove excess enzymes, 25 µL aliquots

(11) Unlocking the Viral Universe: Metagenomic Analysis of Bat Samples Using Next-Generation SequencingMicroorganismsOctober 10, 2023German V. Roev, Nadezhda I. Borisova, Nadezhda V. Chistyakova, Matvey R. Agletdinov, Vasily G. Akimkin, Kamil KhafizovNEBNext® Ultra™ End Repair/dA-Tailing Module (NEB E7546L, Ipswich, MA, USA), NEBNext® Ultra™ Ligation Module (NEB E7595L, Ipswich, MA, USA), Y-shaped adapters compatible with IDT for Illumina Nextera DNA UD Indexes, and NEBNext® Ultra™ II Q5® Master Mix

(12) Two Classes of DNA Gyrase Inhibitors Elicit Distinct Evolutionary Trajectories Toward Resistance in Gram-Negative PathogensbioRxivSeptember 26, 2023Semen A. Leyn, James E. Kent, Jaime E. Zlamal, Marinela L. Elane, Maarten Vercruysse, Andrei L. Ostermansequencing were prepared using a NEBNext Ultra II FS DNA library prep kit for Illumina modules E7810L and E7595L (New England BioLabs) by the manufacturer’s protocol (DNA input ≥100 ng) but using TruSeq DNA PCR-free

(13) Precision digital mapping of endogenous and induced genomic DNA breaks by INDUCE-seqNature CommunicationsDecember 1, 2022Felix M. Dobbs, Patrick van Eijk, Mick D. Fellows, Luisa Loiacono, Roberto Nitsch, Simon H. Reedend-repaired DNA was added directly to the ligation reaction using NEBNext® Ultra™ II Ligation Module (NEB, E7595L) according to the manufacturer’s instructions using 7.5 µM Modified half-functional P7 adapter and omitting

(14) Shared and Unique Evolutionary Trajectories to Ciprofloxacin Resistance in Gram-Negative Bacterial PathogensmBioJune 29, 2021Jaime E. Zlamal, Semen A. Leyn, Mallika Iyer, Marinela L. Elane, Nicholas A. Wong, James W. Wamsley, Maarten Vercruysse, Fernando Garcia-Alcalde, Andrei L. Ostermanclones) were prepared using a NEBNext Ultra II FS DNA library prep kit for Illumina modules E7810L and E7595L (New England BioLabs) by following the manufacturer’s protocol for use with inputs of ≥100 ng, with

(15) Novel CRISPR-based sequence specific enrichment methods for target loci and single base mutationsPLOS ONEDecember 23, 2020Jennifer L. Steele, Richard C. Stevens, Oscar A. Cabrera, Gary J. Bassill, Sabrina M. Cramer, Felipe Guzman, Anthony P. Shuberadded, and UPS adapters were ligated (S2 Table) using the NEBNext Ultra II Ligation Module (NEB, cat. #E7595L). DNA was purified using AMPure XP beads (Beckman Coulter, cat. #A63881) and resuspended. CAMP amplification

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NEBNext Ultra II Ligation Module

NEBNext Ultra II Ligation Module from New England Biolabs

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New England Biolabs

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NEBNext Ultra II Ligation Module from New England Biolabs

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