EnGensgRNA Synthesis Kit,S. pyogenes E3322S from New England Biolabs

Product Specs
ItemEnGensgRNA Synthesis Kit,S. pyogenes
CompanyNew England Biolabs
Price $449.00 Inquire
Catalog NumberE3322S
Quantity20 reactions
ApplicationsFunctional Genomics
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Loss of histone macroH2A1.1 causes kidney abnormalities secondary to a change in nutrient metabolizationSci AdvOctober 24, 2025René Winkler, Gemma Comas-Armangué, David Corujo, Adrián Sanz-Moreno, Julia Calzada-Wack, Shubhra Ashish Bhattacharya, Birgit Rathkolb, Nathalia Romanelli Vicente Dragano, Colina X. Qiao, Valentina Chiodi, et al.The single guide RNAs (sgRNAs) were synthesized using the in vitro transcription EnGen kit (catalog #E3322S, New England Biolabs, MA, USA). DNA oligonucleotides for sgRNA synthesis were generated with the NEB

(2) Tubulin autoregulation factors SCAPER and TTC5 recruit γ-tubulin to non-centrosomal MTOCs for neuronal microtubule nucleation and axon regenerationbioRxivOctober 17, 2025Yi Xie, Alessandro Comincini, Chengye Feng, Carrie Ann Davison, Marc Hammarlund, Francesca Bartolini, Shaul Yogevcontent. The gRNAs were synthesized from DNA oligos using the EnGen sgRNA synthesis kit (NEB catalog #E3322S) and subsequently purified by Monarch RNA cleanup kit (NEB catalog # T2040). The dsDNA repair template

(3) Stable transgene expression and CRISPR-mediated knock-in system of a bacteria-derived antibiotic selection gene in the green alga Ulva proliferaBMC Plant BiolOctober 6, 2025Zheng Qin, Whelver Surnido, Hiroyuki Mizuta, Toshiki Ujiwith a Cas9 protein (New England Biolabs, M0646T) and sgRNA synthesized in vitro (New England Biolabs, E3322S). Briefly, 100 µL of gamete suspension was gently mixed with 100 µL of UTB1. The DNA insert (1 µg) was

(4) Coupling CRISPR scanning with targeted chromatin accessibility profiling using a double-stranded DNA deaminaseNature MethodsOctober 1, 2025Heejin Roh, Simon P. Shen, Yan Hu, Hui Si Kwok, Allison P. Siegenfeld, Ceejay Lee, Marcanthony U. Zepeda, Chun-Jie Guo, Shelby A. Roseman, Caroline Comenho, et al.GGAAACTTTGTTGTCAGACCCGG). Double-stranded DNA was synthesized using the EnGen sgRNA Synthesis Kit (NEB, E3322S) and purified using the Agencourt RNAClean XP system (Beckman Coulter). RNP complexes were assembled,

(5) Universal off-the-shelf combination immunotherapy using oncolytic viruses to redirect T cell engagers to target solid tumorsJ Immunother CancerAugust 11, 2025Anthony K Park, Isabel Monroy, Yuwei Ren, Cathy Lu, Shyambabu Chaurasiya, Hannah Valencia, Jackson Lent-Koop, Colin Cook, Seonah Kang, Lupita Lopez, et al.(5’-GTTATAGTAGCCGCACTCG-3’) was used to synthesize single guide RNA (sgRNA) using the EnGen sgRNA Synthesis Kit (Cat#E3322S; NEB) following the manufacturer’s instruction. Next, ∼70% confluent HEK293 cells in a 24-well plate

(6) Ciliary biology intersects autism and congenital heart diseaseDevelopmentJune 15, 2025Nia Teerikorpi, Kate E. McCluskey, Ethel Bader, Micaela C. Lasser, Sheng Wang, Catherine H. Nguyen, James D. Schmidt, Elina Kostyanovskaya, Nawei Sun, Jeanselle Dea, et al.cells at the two-cell stage for heart phenotyping. For CRISPR experiments, we synthesized (EnGen, NEB E3322S) and purified (Zymo R1018) an sgRNA targeting taok1 or a previously validated control sgRNA targeting

(7) NDR kinase SAX-1 controls dendrite branch-specific elimination during neuronal remodeling in C. elegansbioRxivJune 15, 2025Paola V. Figueroa-Delgado, Shaul Yogevsynthesized sgRNA from single-stranded DNA utilizing the EnGen sgRNA Synthesis Kit, S. pygenes (NEB Cat#E3322S), followed by sgRNA purification utilizing the Monarch RNA cleanup kit (NEB #T2040). A detailed list

(8) Loss of Ten1 in mice induces telomere shortening and models human dyskeratosis congenitaSci AdvApril 11, 2025Adrián Sanz-Moreno, Lore Becker, Kan Xie, Patricia da Silva-Buttkus, Nathalia R. V. Dragano, Antonio Aguilar-Pimentel, Oana V. Amarie, Julia Calzada-Wack, Markus Kraiger, Stefanie Leuchtenberger, et al.(sgRNAs) were synthesized by using the in vitro transcription EnGen Kit [New England Biolabs (NEB), E3322S]. DNA oligonucleotides for the sgRNA synthesis were generated with the NEB tool (http://nebiocalculator.neb.com/#!/sgrna)

(9) Cas9 endonuclease: a molecular tool for in vitro cloning and CRISPR edit detectionFront Genome EdApril 1, 2025Xingliang Ma, Dhouha Kthiri, Manpartik S. Gill, Curtis J. Pozniak, Sateesh Kagalepreparation The sgRNAs were synthesized in vitro using either the EnGen sgRNA Synthesis Kit (NEB, catalog no. E3322S) or the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB, catalog no. E2050S), according to the manufacturer’s

(10) Autism gene variants disrupt enteric neuron migration and cause gastrointestinal dysmotilityNat CommunMarch 6, 2025Kate E. McCluskey, Katherine M. Stovell, Karen Law, Elina Kostyanovskaya, James D. Schmidt, Cameron R. T. Exner, Jeanselle Dea, Elise Brimble, Matthew W. State, A. Jeremy Willsey, et al.compared against 5.5 ng of the standard control morpholino. For CRISPR, we synthesized (EnGen, NEB E3322S) and purified (Zymo R1018) validated sgRNAs6 and injected 400 pg of sgRNA and 2.24 ng of Cas9-NLS protein

(11) Pioneering genome editing in parthenogenetic stick insects: CRISPR/Cas9-mediated gene knockout in Medauroidea extradentataSci RepJanuary 20, 2025Giulia Di Cristina, Elina Dirksen, Benjamin Altenhein, Ansgar Büschges, Sigrun I. Korschingoligo (GTTTTAGAGCTAGA). SgRNAs were synthesized with the EnGen® sgRNA Synthesis Kit, S. pyogenes (NEB E3322S) and purified using the Monarch® RNA Cleanup Kit (50 µg) (NEB T2040L). To test sgRNA in-vitro efficiency,

(12) The white gene as a transgenesis marker for the cricket Gryllus bimaculatusG3: Genes, Genomes, GeneticsOctober 15, 2024Emmanuel Gonzalez-Sqalli, Matthieu Caron, Benjamin Loppin2015). sgRNAs were synthesized using the EnGen sgRNA Synthesis Kit, S. pyogenes (New England BioLabs #E3322S) and purified using Monarch RNA Cleanup Kit (New England Biolabs #T2040L), following manufacturer's

(13) Editing of endogenous tubulins reveals varying effects of tubulin posttranslational modifications on axonal growth and regenerationeLifeJuly 1, 2024Yu-Ming Lu, Shan Yan, Shih-Chieh Ti, Chaogu Zheng(https://chopchop.cbu.uib.no/). The sgRNAs were synthesized using the NEB EnGen sgRNA Synthesis Kit (E3322S) and purified using NEB Monarch RNA Cleanup Kit (T2030L). One μg of the sgRNA was complexed with 20

(14) Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transportJournal of Cell BiologyMay 6, 2024Youjun Wu, Chen Ding, Behrang Sharif, Alexis Weinreb, Grace Swaim, Hongyan Hao, Shaul Yogev, Shigeki Watanabe, Marc Hammarlundal., 2023). This gRNA was synthesized from ssDNA using the EnGen sgRNA Synthesis Kit, S. pygenes (#E3322S; NEB), and subsequently purified using a Monarch RNA cleanup kit (#T2040; NEB). All repair templates

(15) A synthetic biology approach to assemble and reboot clinically relevant Pseudomonas aeruginosa tailed phagesMicrobiology SpectrumMarch 5, 2024Thomas Ipoutcha, Ratanachat Racharaks, Stefanie Huttelmaier, Cole J. Wilson, Egon A. Ozer, Erica M. Hartmannthe Streptococcus pyogenes CRISPR system. sgRNA was produced using EnGen sgRNA Synthesis Kit (NEB, E3322S), primer D31 or D32, and purified using Monarch RNA Cleanup Kit (NEB, T2040S). Cas9 nuclease (NEB, M0386S),

(16) Functional dissection of human cardiac enhancers and noncoding de novo variants in congenital heart diseaseNature GeneticsMarch 1, 2024Feng Xiao, Xiaoran Zhang, Sarah U. Morton, Seong Won Kim, Youfei Fan, Joshua M. Gorham, Huan Zhang, Paul J. Berkson, Neil Mazumdar, Yangpo Cao, et al.interest were designed using CHOPCHOP46 and transcribed in vitro using the EnGen sgRNA Synthesis Kit (NEB, E3322S). Oligonucleotide sequences are in Supplementary Table 5. WTC-Cas9 iPS cells were treated with 2 μg

(17) Revisiting chromatin packaging in mouse spermGenome researchDecember 27, 2023Yin Q, Yang CH, Strelkova OS, Wu J, Sun Y, Gopalan S, Yang L, Dekker J, Fazzio TG, Li XZ, et al.(https://bitbucket.org/valenlab/chopchop/src/master/) and purchased from IDT. sgRNA were prepared using EnGen sgRNA synthesis kit (NEB E3322S). RNP complexes were assembled individually by combining 8 µL of nuclease-free water, 1.5 µL of NEBuffer

(18) A kinesin-1 adaptor complex controls bimodal slow axonal transport of spectrin in Caenorhabditis elegansDevelopmental CellOctober 1, 2023Oliver Glomb, Grace Swaim, Pablo Munoz Munoz LLancao, Christopher Lovejoy, Sabyasachi Sutradhar, Junhyun Park, Youjun Wu, Sydney E. Cason, Erika L.F. Holzbaur, Marc Hammarlund, et al.of 20 μl. The gRNA was synthesized from ssDNA using the EnGen sgRNA Synthesis Kit, S. pygenes (NEB# E3322S) and subsequently purifying the gRNA using a Monarch RNA cleanup kit (NEB#T2040). In case of dsDNA as

(19) Variation of extrachromosomal circular DNA in cancer cell linesComputational and Structural Biotechnology JournalAugust 28, 2023Carl Rung dos Santos, Lasse Bøllehuus Hansen, Monica Rojas-Triana, Astrid Zedlitz Johansen, Mirna Perez-Moreno, Birgitte Regenberg(cat.no. M0386M, NEB). sgRNAs were synthesized using the EnGen sgRNA Synthesis Kit, S. pyogenes (cat.no. E3322S, NEB) according to the manufacturer’s protocol. Mouse-sgRNA1: GTAGCATGAACGGCTAAACGA. Mouse-sgRNA2: GGCCTGATAATAGTGACGCT.

(20) Polarized localization of kinesin-1 and RIC-7 drives axonal mitochondria anterograde transportbioRxivJuly 18, 2023Youjun Wu, Chen Ding, Alexis Weinreb, Laura Manning, Grace Swaim, Shaul Yogev, Daniel A. Colón-Ramos, Marc Hammarlundwas used. This gRNA was synthesized from ssDNA using the EnGen sgRNA Synthesis Kit, S. pygenes (NEB# E3322S) and subsequently purified using a Monarch RNA cleanup kit (NEB#T2040). All repair templates contained

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EnGensgRNA Synthesis Kit,S. pyogenes

EnGensgRNA Synthesis Kit,S. pyogenes from New England Biolabs

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New England Biolabs

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EnGensgRNA Synthesis Kit,S. pyogenes from New England Biolabs

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