NEBNextUltraII Directional RNA Library Prep Kit for Illumina E7760L from New England Biolabs

Product Specs
ItemNEBNextUltraII Directional RNA Library Prep Kit for Illumina
CompanyNew England Biolabs
Price $3,239.00 Inquire
Catalog NumberE7760L
Quantity96 reactions
ApplicationsDNA Analysis, Library Prep, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Meta-transcriptomic profiling of Ixodes ricinus and Ixodes persulcatus ticks from Finland reveals diverse RNA viromes, community structuring, and emerging viral lineagesArch VirolSeptember 21, 2026Theophilus Yaw Alale, Jesse Mänttäri, Heidi M. Viitaniemi, Mikhail Ozerov, Miguel Baltazar-Soares, Jani J. Sormunen, Eero J. VesterinenrRNA-depleted RNA using the NEBNext® Ultra II Directional RNA Library Prep Kit (New England Biolabs, #E7760L). RNA fragmentation, first- and second-strand cDNA synthesis, adapter ligation, and PCR enrichment were

(2) Derivation of Mouse Dome-Shaped Trophoblast Stem Cells With Low Heterogeneity From BlastocystsCell ProlifSeptember 18, 2026Minli Liu, Shaofang Ren, Xinyu Xia, Guanlin Lu, Haojie Yang, Yi Zheng, Jiexiang Zhao, Xiao-Yang Zhao, Mei Wangaccording to the manufacturer's instructions with the NEB Next Ultra II Directional RNA Library Prep Kit (E7760L, NEB). The constructed NGS libraries were sequenced with the Illumina Hiseq XTEN platform at Novogene

(3) Reorientation of the SUMOylation landscape and altered L3mbtl2-mediated transcriptional activity in cancer-associated muscle contractile dysfunctionCell Death DiscovSeptember 9, 2026Luis Vincens Gand, Mugeng Li, Katharina Brandt, Baoyu Zhou, Amel Nassar-Grioua, Julien Gondin, Edgar Becker, Andreas Pich, Theresia Kraft, Mamta Amrute-Nayak, et al.stranded cDNA library generation using ‘NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina’ (E7760L; New England Biolabs) according to the manualE7760 (Version 1.0_02-2017; NEB), except that reactions

(4) Tau Aggregate Imaging and Transcriptomics of Alzheimer's Disease Brain at Different Stages of DiseaseAdvanced ScienceAugust 31, 2026Elizabeth A. English, Dorothea Böken, Matthew W. Cotton, Matthew R. Cheetham, Johanna S. Jackson, Georg Meisl, John S. H. Danial, Paul M. Matthews, Nurun N. Fancy, David Klenerman(E7400X). Genomic Libraries were constructed using the New England Biolabs Library construction kit (E7760L), using the NEBNext Multiplex oligos for Illumina (E6442S). Libraries were quantified using a Qubit

(5) Fibrin Degradation Products Regulate Endothelial Barrier Function in Traumatic Brain Injury via the Actin CytoskeletonNeurotrauma RepAugust 26, 2026Ayhan Atmanli, Aidan Michael Collier, Scott Douglas Olson, Charles Samuel CoxModule (E7490L, New England Biolabs), NEBNext Ultra II Directional RNA Library Prep Kit for ILLumina (E7760L, New England Biolabs) and NEBNext Multiplex Oligos for Illumina (E6609S, New England Biolabs) following

(6) AI-2 production in Fusobacterium nucleatum is subspecies-specific and uncoupled from quorum sensingJournal of BacteriologyAugust 20, 2026Bibek G C, Shiqi Xu, Truman Tran, Chenggang WuStrand-specific libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L, New England Biolabs) together with NEBNext Multiplex Oligos for Illumina (E6609S, New England Biolabs),

(7) NSUN2 Promotes Cancer Immune Evasion via its Moonlighting Function Acting on Metabolic ReprogrammingCancer CommunicationsAugust 19, 2026Baoxiang Chen, Yanrong Deng, Xiang Zhai, Peiyun Wang, Kunming Lei, Siyuan Yin, Quanjiao Chen, Qun Qian, Jinfang Zhang, Xianghai Ren, et al.reverse-transcribed into complementary DNA (cDNA) using the NEBNext Ultra RNA Library Prep Kit for Illumina (E7760L, New England Biolabs). Purified double-stranded cDNA fragments were end-repaired, A-tailed, and ligated

(8) The WhiA and WhiB2 transcription factors mediate the Mycobacterium tuberculosis cell cycle by regulating DNA replication and cell divisionNAR Mol MedAugust 4, 2026Frances Marks, Mirjana Lilic, Pranav Nalam, Leah Mclelland, Xiaoxian An, Sarah M Fortune, Elizabeth A Campbell, Sabine Ehrtwere prepared using NEBNext Ultra II Directional RNA Library Prep Kit of Illumina (New England Biolabs E7760L), according to instructions for 150 ng of input mRNA. Samples were sequenced generating paired 5 million

(9) High-diversity base mutagenesis via simultaneous adenine, cytosine and guanine editingNature CommunicationsJuly 27, 2026Mengjia Hong, Changming Luan, Meng Yuan, Hao Huang, Xinyuan Guo, Dihao Meng, Molin Huang, Ye Xu, Shanghui Zhao, Ke Chen, et al.performed using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs; E7760L) according to the manufacturer’s instructions. Barcoded libraries were clustered on a cBot system using

(10) Age dependent collective trafficking of tissue resident T cells in zebrafishbioRxivJuly 15, 2026Udochi F. Azubuike, Patricia B Gordon, Khanh Loan Ly, Woong Young So, Lanie Le, Kevin Bishop, Raman Sood, Michael Kruhlack, Michael M. Gottesman, Kandice TannerIndex Barcodes (New England Biolabs #E6440S), and Directional RNA Library Kit (New England Biolabs #E7760L). 150bp fragments were sequenced by paired-end 75bp utilizing Illumina NextSeq 2000 P4 XLEAP-SBS Reagent

(11) Defined bacterial consortium highlights the impact of intestinal bacteria on DNA methylation and tumorigenesisGenome BiolJuly 7, 2026Claudia Mercado-Rodriguez, Siddhi Chitre, Pyoung Hwa Park, Ye Yang, Anthony Pompetti, Raad Z. Gharaibeh, Jason O. Brant, Jean-Pierre J. Issa, Christian Jobinlibrary construction was performed with the NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760L) according to the manufacturer's user guide. Briefly, 250 ng of total RNA was used for mRNA isolated

(12) Low PEEP ventilation in TGF-β1 induced lung injury triggers a reversible lung mechanical deterioration without promoting persistent structural damageSci RepJuly 7, 2026Franziska Roeder, Lara-Kristin Steinmetz, Martin Kolb, Ulrich A. Maus, Bradford J. Smith, Lars Knudsenstranded cDNA libraries employing the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (E7760L; New England Biolabs). All procedures were carried out according to the manufacturer’s protocol (User

(13) Ribo-Tweezer: Rapid removal of ribosomal proteins reveals additional layers of post-transcriptional gene regulationMolecular CellJuly 2, 2026Yuxiang Chen, Ching Pin Cheng, Kitra Cates, Georgi K. Marinov, Travis C. Lantz, Haojun Yang, Isabelle Liu, Naomi R. Genuth, Christina Andronescu, Victoria Hung, et al.ProteoExtract Protein Precipitation Kit,Millipore,Cat#539180 NEBNext Ultra II Directional RNA Library Prep Kit for Illumina,New England Biolabs,Cat#E7760L ERCC RNA Spike-In Mix,Thermo Fisher,Cat#4456740 DNA Clean & Concentrator-5,Zymo Research,Cat#D4003 CircLigase

(14) The importance of HPV testing in oropharyngeal cancer in two US populationsNeoplasiaJuly 2, 2026M.M. Lawas, M. Hsieh, S. Patil, E. Mohebbi, R. Zhang, J. Ahn, W. Benjamin, S.B. Chinn, M. Witek, B. Mabvakure, et al.strand-specific library preparation using the NEBNext Ultra II Directional RNA Library Prep Kit (NEB, Cat# E7760L). For batches 9067-MS and 10268-MS, ribosomal RNA depletion was performed using the NEBNext rRNA Depletion

(15) Expanding the spectrum of non-canonical NUT carcinoma: clinicopathological and molecular characterization of BRD3::NUTM1 and WWTR1::NUTM1 fusion variantsFront OncolJuly 1, 2026Zuoyu Liang, Chan Yang, Xiaoqian Zhai, Yan Wang, Min Chen, Mei Tang, Linlin Huang, Mengqian Li, Ping Zhou, Xuexue Wu, et al.library preparation were performed using NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB, E7760L). RNA-seq libraries were sequenced on the Gene + Seq-2000 with a paired-end 2 × 100 bp protocol, resulting

(16) The mutational landscape of STING-induced immunityNatureJune 24, 2026Bing Zhang, Pengbiao Xu, Yu Meng, Laure Gallay, François Lestelle, Hélène Morel, Marie-Louise Frémond, Bruno E. Correia, Andrea Ablassersamples were prepared with NEBNext Ultra II Directional RNA Library Prep Kit (New England Biolabs, E7760L) with ribodepletion starting from 750 ng of RNAs. The quality control was performed using Qubit DNA

(17) Serotonin 5-HT7 receptor signaling modulates inflammatory responses and survival after myocardial infarctionJ Transl MedJune 17, 2026Franziska E. Müller, Frauke S. Bahr, Sergej Erschow, Martina Kasten, Maren Heimerl, Nils Benen, Mira Jung, Arne Schmidt, Michaela Scherr, Christine S. Falk, et al.generation using ‘NEBNext® Ultra II Directional RNA Library Prep Kit for Illumina’ (New England Biolabs, E7760L). All steps were performed as recommended in user manualE7760 (Version 1.0_02-2017; NEB) except that

(18) Germline sequence variation within the ribosomal DNA is associated with human complex traitsCell GenomicsJune 10, 2026Francisco Rodriguez-Algarra, Elliott Whittaker, Maia Cooper, Sergey Koren, Maria R. Conte, Adam M. Phillippy, Faraz K. Mardakheh, David M. Evans, Vardhman K. Rakyanassays ,, NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina®,New England Biolabs,Cat#E7760L ,, Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines ,, Human

(19) An X-to-autosome-to-Y chromosome amplified retrogene family functions in spermatidsCurrent BiologyJune 8, 2026Ivan F. Mier, Martin F. Arlt, Ann Marie Lawson, Eden A. Dulka, T. Brock Wooldridge, Hopi E. Hoekstra, Jacob L. MuellerLibraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7760L), and unique i5 and i7 Illumina indices were annealed through PCR amplification (NEBNext Multiplex Oligo

(20) Multiomic characterization of malignant pulmonary nodules and development of a methylation-based diagnostic ModelJ Transl MedJune 8, 2026Wei Zhou, Tongji Xie, Qingjun Wu, Mengmeng Song, Jianzhen Weng, Jieli Zhang, Xuan Gao, Min Zheng, Zheng Wang, Yunzhi Zhou, et al.library preparation were performed using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (NEB#E7760L). The library was quantitated using a Qubit 3.0 instrument (Life Invitrogen, USA), and its quality was

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NEBNextUltraII Directional RNA Library Prep Kit for Illumina

NEBNextUltraII Directional RNA Library Prep Kit for Illumina from New England Biolabs

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NEBNextUltraII Directional RNA Library Prep Kit for Illumina from New England Biolabs

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