NEBNext Single Cell/Low Input cDNA Synt E6421S from New England Biolabs

Product Specs
ItemNEBNext Single Cell/Low Input cDNA Synt
CompanyNew England Biolabs
Price $673.00 Inquire
Catalog NumberE6421S
Quantity24 reactions
ApplicationsDNA Analysis, NGS
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Protocol for efficient CRISPRi-mediated silencing of retrotransposons in human pluripotent stem cellsSTAR ProtocolsMarch 20, 2026Anita Adami, Raquel Garza, Fereshteh Dorazehi, Christopher H. Douse, Johan Jakobsson7962347001 NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module,New England Biolabs,Cat#: E6421S for 24 reactions or E6421L for 96 reactions Bioanalyzer High Sensitivity kit,Agilent,NA ,, Deposited

(2) miR-203 facilitates timely cell fate transitions via epigenetic modulation during early embryogenesisSci AdvMarch 13, 2026José González-Martínez, Agustín Sánchez-Belmonte, Estefanía Ayala, Beatriz Escobar, Jesús Gómez, Alejandro García-Díaz, Enrique Nogueira, Jaime Muñoz, Anna Melati, Andreas Brandauer, et al.low-input lysis buffer of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification module (#E6421S). Whole cells were processed with the “NEBNext Single Cell/Low Input RNA Library Prep” kit [New England Biolabs

(3) LINE-1 retrotransposons mediate cis-acting transcriptional control in human pluripotent stem cells and regulate early brain developmentCell GenomicsOctober 8, 2025Anita Adami, Raquel Garza, Patricia Gerdes, Pia A. Johansson, Fereshteh Dorazehi, Symela Koutounidou, Laura Castilla-Vallmanya, Diahann A.M. Atacho, Yogita Sharma, Jenny G. Johansson, et al.130-115-660 NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module,New England Biolabs,Cat#: E6421S for 24 reactions or E6421L for 96 reactions Qubit dsDNA HS kit,Invitrogen(ThermoFisher),Cat# Q32851 Bioanalyzer

(4) Accurate fusion transcript identification from long- and short-read isoform sequencing at bulk or single-cell resolutionGenome ResearchApril 14, 2025Qian Qin, Victoria Popic, Kirsty Wienand, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Christophe Georgescu, Catarina D. Campbell, Arthur Dondi, et al.components from a NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs E6421S). The RNA Samples were diluted, the cancer cell lines to 50 ng/µL, and the SeraSeq fusion RNA mix to

(5) Protocol for enhancing RNA yield and quality from single nuclei isolated from mouse brain tissueSTAR ProtocolsDecember 20, 2024Arif Murat Kocabas, Isaac Marin-ValenciaKit,QIAGEN,Cat# 74004 NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module,NEB,Cat# E6421S LightCycler 480 SYBR Green I Master,Roche,Cat# 4707516001 LightCycler 480 Multiwell plate 96,Roche,Cat#

(6) Ing4-deficiency promotes a quiescent yet transcriptionally poised state in hematopoietic stem cellsiScienceAugust 16, 2024Thompson Z, Anderson GA, Hernandez M, Alfaro Quinde C, Marchione A, Rodriguez M, Gabriel S, Binder V, Taylor AM, Kathrein KL."LIVE/DEAD™ Fixable Red Dead Cell Stain Kit, for 488 nm excitation",Thermofisher,Cat#L34971 NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Kit,New England Biolabs,Cat#E6421S MitoProbe™ TMRM Assay Kit for Flow Cytometry,Thermofisher,M20036 ,, Deposited data,Deposited data,Deposited

(7) Transcriptional determinism and stochasticity contribute to the complexity of autism-associated SHANK family genesCell ReportsJuly 23, 2024Xiaona Lu, Pengyu Ni, Paola Suarez-Meade, Yu Ma, Emily Niemitz Niemitz Forrest, Guilin Wang, Yi Wang, Alfredo Quiñones-Hinojosa, Mark Gerstein, Yong-hui JiangBuffer,PACBIO,PN 101–633–500 NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module,NEB,Cat#: E6421S NEBNext® High-Fidelity 2X PCR Master Mix,NEB,Cat#: M0541S ProNex® Beads,Promega,Cat #: NG2001 Qubit® dsDNA HS Assay Kit

(8) Imaging brain glucose metabolism in vivo reveals propionate as a major anaplerotic substrate in pyruvate dehydrogenase deficiencyCell MetabolismJune 4, 2024Isaac Marin-Valencia, Arif Kocabas, Carlos Rodriguez-Navas, Vesselin Z. Miloushev, Manuel González González-Rodríguez, Hannah Lees, Kelly E. Henry, Jake Vaynshteyn, Valerie Longo, Kofi Deh, et al.Kit,Qiagen,Cat# 74004 NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module,NEB,Cat# E6421S Invitrogen superscript IV First Strand Synthesis System,Thermo Fisher,Cat# 18091050 LightCycler 480 SYBR Green I Master

(9) CTAT-LR-fusion: accurate fusion transcript identification from long and short read isoform sequencing at bulk or single cell resolutionbioRxivFebruary 29, 2024Qian Qin, Victoria Popic, Houlin Yu, Emily White, Akanksha Khorgade, Asa Shin, Kirsty Wienand, Arthur Dondi, Niko Beerenwinkel, Francisca Vazquez, et al.NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, cat. no. E6421S). The RNA Samples were diluted, the cancer cell lines to 50 ng/μl, and the SeraSeq fusion RNA mix to

(10) LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversificationScience AdvancesNovember 3, 2023Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski, et al.October 2019) using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (catalog nos. E6421S for 24 reactions and E6421L for 96 reactions, New England Biolabs), the Iso-Seq Express Oligo Kit (catalog

(11) Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflowsPlant MethodsOctober 21, 2023Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem, Robert J. Henryof the Iso-Seq library, the NEBNext Single Cell/Low Input cDNA Synthesis, Amplification Module (NEB, E6421S), and the SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900) were used based on the standard

(12) Einkorn genomics sheds light on history of the oldest domesticated wheatNatureAugust 2, 2023Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero, et al.separately using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, E6421S). The ProNex Size-Selective Purification System (Promega, NG2001) was used for size selection. One SMRT

(13) Direct haplotype-resolved 5-base HiFi sequencing for genome-wide profiling of hypermethylation outliers in a rare disease cohortNature CommunicationsMay 29, 2023Warren A. Cheung, Adam F. Johnson, William J. Rowell, Emily Farrow, Richard Hall, Ana S. A. Cohen, John C. Means, Tricia N. Zion, Daniel M. Portik, Christopher T. Saunders, et al.cDNA synthesis with a NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, Cat. No. E6421S) and an Iso-Seq Express Oligo Kit (PacBio, Cat. No. 101-737-500) as described in the Iso-Seq Express

(14) The gyrfalcon (Falcon rusticolus) genomeG3 Genes|Genomes|GeneticsMarch 9, 2023Andrea Zuccolo, Sara Mfarrej, Mirko Celii, Saule Mussurova, Luis F Rivera, Victor Llaca, Nahed Mohammed, Arnab Pain, Abdulmajeed Fahad Alrefaei, Abdulwahed Fahad Alrefaei, et al.was done using NEBNext Single Cell/Low input cDNA synthesis and amplification module (NEB, Cat No.: E6421S) with Iso-Seq Express Oligo Kit (Pacbio, 101-737-500) and Iso-seq library was built with SMRTbell Express

(15) A chromosome-level reference genome and pangenome for barn swallow population genomicsCell ReportsJanuary 31, 2023Simona Secomandi, Guido R. Gallo, Marcella Sozzoni, Alessio Iannucci, Elena Galati, Linelle Abueg, Jennifer Balacco, Manuela Caprioli, William Chow, Claudio Ciofi, et al.NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs, cat. no. E6421S) and Iso-Seq Express Oligo Kit (PacBio PN 10 1-737-500) from 300 ng of total RNA for both brain and

(16) Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcomaNature cancerAugust 1, 2022Wei Y, Qin Q, Yan C, Hayes MN, Garcia SP, Xi H, Do D, Jin AH, Eng TC, McCarthy KM, et al.the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs # E6421S). Amplified cDNA was then used in each quantitative PCR (PowerUp SYBR Green Master Mix, Fisher Scientific

(17) Long-read genome sequencing of bread wheat facilitates disease resistance gene cloningNature GeneticsMarch 14, 2022Naveenkumar Athiyannan, Michael Abrouk, Willem H. P. Boshoff, Stéphane Cauet, Nathalie Rodde, David Kudrna, Nahed Mohammed, Jan Bettgenhaeuser, Kirsty S. Botha, Shannon S. Derman, et al.using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, E6421S) and the ProNex Size-Selective Purification System (Promega, NG2001) for size selection. In brief, 300

(18) NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cellsmAbsJanuary 1, 2022Subas Satish HP, Zeglinski K, Uren RT, Nutt SL, Ritchie ME, Gouil Q, Kluck RM.cells were prepared using the NEBNext® single cell/low input cDNA synthesis and amplification module (E6421S; New England BioLabs Inc.). As a single mammalian cell contains only 0.1 pg mRNA,42 this module is required

(19) Single worm transcriptomics identifies a developmental core network of oscillating genes with deep conservation across nematodesGenome ResearchSeptember 1, 2021Shuai Sun, Christian Rödelsperger, Ralf J. Sommerliquid nitrogen. Afterward, 3 µL of lysis buffer (low input cDNA synthesis & amplification module; NEB E6421S) was added, and samples were incubated for 40 min at 65°C, followed by 1 min at 85°C. To remove genomic

(20) Identification of neural progenitor cells and their progeny reveals long distance migration in the developing octopus braineLifeAugust 24, 2021Deryckere A, Styfhals R, Elagoz AM, Maes GE, Seuntjens E.kit,NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module,New England BioLabs.,Cat#: E6421S, Commercial assay or kit,Superscript III Reverse Transcriptase,Invitrogen,Cat#: 18080–044, Commercial

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NEBNext Single Cell/Low Input cDNA Synt

NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs

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New England Biolabs

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NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs

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