NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads E7765S from New England Biolabs

Product Specs
ItemNEBNextUltraII Directional RNA Library Prep with Sample Purification Beads
CompanyNew England Biolabs
Price $1,056.00 Inquire
Catalog NumberE7765S
Quantity24 reactions
ApplicationsDNA Analysis, Library Prep, NGS
TemperatureMulti-Temperature

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) Multi-omic phenotyping of MAPT V337M neurons reveals early changes in axonogenesis and tau phosphorylationNPJ DementApril 25, 2026Gregory A. Mohl, Gary Dixon, Emily Marzette, Justin McKetney, Avi J. Samelson, Carlota Pereda Serras, Julianne Jin, Nabeela Ariqat, Andrea Keys, Cristian Chavira, et al.No. E7405L). cDNA synthesis was then performed on all remaining RNAs (New England BioLabs, Cat. No. E7765S). Paired-end (PE65) sequencing was performed at the Chan Zuckerberg Biohub and the UCSF Center for Advanced

(2) Epstein-Barr Virus Latent Membrane Protein 1 Suppresses Ferroptosis via Pentose Phosphate Pathway and Glutathione MetabolismbioRxivMarch 13, 2026Eric M. Burton, Bidisha Mitra, Rui Guo, John M. Asara, Benjamin E. Gewurz(Cat#E7490S), and library preparation with NEBNext Ultra RNA Library Prep with Sample Purification Beads (Cat#E7765S). Each experimental treatment was performed in biological triplicate. Libraries were multi-indexed (NEB

(3) CX-5461 and doxorubicin activate a shared DNA damage-associated transcriptional response in human cardiomyocytesG3: Genes, Genomes, GeneticsMarch 4, 2026Sayan Paul, José A Gutiérrez, Alyssa R Bogar, E Renee Matthews, Michelle C Wardprepared using the NEBNext Ultra II Directional RNA Library Prep Kit with Sample Purification Beads (E7765S; NEB) and indexed using NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs, E6440S). Sub-micromolar

(4) Human CRAMP1 specifically promotes the expression of histone H1 genesEMBO ReportsFebruary 9, 2026Justin Bodner, Pranathi Vadlamani, Alexander S Lee, Kathryn A Helmin, Qianli Liu, Almira E Pratasenia, Maria M A Horst, Sudharsana Ravisankar, Sakshi Khurana, Marc L Mendillo, et al.Protein A Dynabeads,Thermo Fisher,10001D NEBNext Ultra II DNA Library Preparation kit,New England Biolabs,E7765S NEBNext Unique Dual Index Primers,New England Biolabs,E6440S NEBNext rRNA Depletion Kit V2,New England Biolabs

(5) Circular RNA Pde4dip regulates myogenesis by interacting with Zfp143 mRNA: a novel regulatory axisRNA BiologyDecember 31, 2025Suman Singh, Arundhati Das, Gaurahari Sahoo, Amaresh Chandra PandaCrosslinking-Immunoprecipitation-Sequencing (CLiPP-Seq). The cDNA libraries were prepared using the NEB NeXT Ultra II Library Prep Kit (NEB, E7765S) with minor modifications to incorporate additional steps of de-crosslinking at 254 nm after RNA fragmentation

(6) Widespread association of Polycomb complex–deposited histone H2A monoubiquitylation with enhancers and neuronal gene regulationScience AdvancesNovember 28, 2025Kailynn MacGillivray, Daniel Fusca, Luomeng Tan, Reta Aram, Arneet L. Saltzmanprepared with the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S) according to the manufacturer’s directions with NEBNext Multiplex Oligos for Illumina (NEB E6440).

(7) Molecular Adaptations to Repeated Radiation Exposure in Triple-Negative Breast Cancer: Dysregulation of Cell Adhesion, Mitochondrial Function, and Epithelial-Mesenchymal TransitionInt J Mol SciOctober 1, 2025Noah Dickinson, Alyssa Murray, Megan Davis, Kaitlyn Marshall-Bergeron, Jessica Dougherty, Wuroud Al-Khayyat, Ramya Narendrula, Maggie Lavoie, Emma Mageau, Ronan Derbowka, et al.Cat. #: T2040L). The NEBNext® Ultra™ II Directional RNA Library Kit (NEB, Ipswich, MA, USA, Cat. #: E7765S) was then used for library preparation. This kit isolated the poly-A messenger ribonucleic acid (mRNA)

(8) FRA1 (FOSL1) suppresses neoplastic transformation and modulates radiation responses via transcriptional control of mitogenic and stress-responsive networksFrontiers in Cell and Developmental BiologySeptember 22, 2025Wuroud Al-Khayyat, Taylor Laframboise, Jessica Dougherty, Marc S. Mendonca, Douglas R. Boreham, T. C. Tai, Christopher Thome, Sujeenthar Tharmalingamto generate strand-specific libraries using the NEBNext® Ultra™ II Directional RNA Library Prep Kit (E7765S, NEB), which enriches for poly-A mRNA and incorporates the dUTP method for strand specificity. Libraries

(9) Overcoming Ferroptosis-Induced Exhaustion of NK Cells through Inhibition of the ATF3-Mediated Integrated Stress Response in Ovarian CancerInternational Journal of Biological SciencesAugust 30, 2025Tian Chen, Shulin Zhou, Yashuang Zhang, Huangyang Meng, Huixian Miao, Mingming Feng, Yi Jiang, Yicong Wan, Lin Zhang, Wenjun Chengprobes, libraries were constructed using the Ultra™ II Directional RNA Library Prep Kit for Illumina (E7765S, NEB, MA) and then sequenced on the Illumina HiSeq X Ten sequencing platform at Novogene Biotechnology,

(10) Exposing the DNA methylation-responsive compartment of the leukaemic genome in T-ALL cell lines support its potential as a novel therapeutic target in T-ALLClin EpigeneticsJuly 3, 2025Maike Bensberg, Aida Selimović-Pašić, Lisa Haglund, Júlia Goldmann, Sandra Hellberg, Colm E. Nestor(New England Biolabs, E7405L), NEBNext Ultra II Directional RNA Library Prep (New England Biolabs, E7765S), and NEBNext Multiplex Oligos for Illumina (New England Biolabs, E7335S) according to the manufacturer’s

(11) Reversible proliferative arrest induced by rapid depletion of RNase MRPNat CommunJune 18, 2025Yuan Liu, Shiyang He, Kawon Pyo, Reuben Franklin, Ibrahim B. Maaz, Chen Cai, Kriti Shah, Sihem Cheloufi, William F. Marzluff, Jernej Murnprepared using the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB E7765S). RNA-seq libraries were prepared, sequenced, and analyzed in triplicate. The libraries were subject

(12) The RNA content of extracellular vesicles from gene-edited PRPF31 +/- hiPSC-RPE show potential as biomarkers of retinal degenerationMolecular Therapy Methods & Clinical DevelopmentJune 12, 2025Heran Getachew, Sudeep Mehrotra, Tarandeep Kaur, Rosario Fernandez-Godino, Eric A. Pierce, Marcela Garita-Hernandezusing the NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (catalog no. E7765S, New England Biolabs, Ipswich, MA), NEBNext Poly(A) mRNA Magnetic Isolation Module (catalog no. E7490S,

(13) Physiological roles of an Acinetobacter -specific σ factormBioJune 11, 2025Emily E. Bacon, Kevin S. Myers, Rubén Iruegas-López, Amy B. Banta, Michael Place, Ingo Ebersberger, Jason M. Petersprepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). Libraries were sequenced on Illumina NovaSeq

(14) SCORT–Cas13d Nanotherapy Precisely Targets the ‘Undruggable’ Transcription Factor HoxB13 in Metastatic Prostate Cancer In VivoAdvanced ScienceMay 11, 2025Zhifen Cui, Furong Huang, Kun Fang, Jingyue Yan, Yuebao Zhang, Diana D. Kang, Yufan Zhou, Yue Zhao, Jeffrey I. Everitt, William Hankey, et al.library construction with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, no. E7765S) according to the manufacturer's instructions. Complementary DNA molecules were amplified for eight

(15) Bacteria use exogenous peptidoglycan as a danger signal to trigger biofilm formationNat MicrobiolJanuary 3, 2025Sanika Vaidya, Dibya Saha, Daniel K. H. Rode, Gabriel Torrens, Mads F. Hansen, Praveen K. Singh, Eric Jelli, Kazuki Nosho, Hannah Jeckel, Stephan Göttig, et al.carried out using NEBNext Ultra II Directional RNA Library Prep with Sample Purification Beads (NEB, E7765S). Sequencing was carried out at the Max Planck Genome Centre (Cologne, Germany) using an Illumina HiSeq3000

(16) Rapid design of bacteriophage cocktails to suppress the burden and virulence of gut-resident carbapenem-resistant Klebsiella pneumoniaeCell Host & MicrobeNovember 13, 2024Ella Rotman, Sandra McClure, Joshua Glazier, Jay Fuerte-Stone, Jonathan Foldi, Ali Erani, Rory McGann, Jack Arnold, Huaiying Lin, Sandra Valaitis, et al.kit,Qiagen,Cat. No. / ID: 74106 NEBNext® Ultra™ II Directional RNA Library Prep Kit,New England Biolabs,NEB: E7765S LunaScript RT SuperMix Kit,New England Biolab,E3010L "Zymo RNA Clean & Concentrator-5 with DNase I,

(17) Biosafety Evaluation of a Chimeric Adenoviral Vector in Mini-Pigs: Insights into Immune Tolerance and Gene Therapy PotentialBiomedicinesNovember 9, 2024Andrei Izmailov, Irina Minyazeva, Vage Markosyan, Zufar Safiullov, Ilnaz Gazizov, Ilnur Salafutdinov, Maria Markelova, Ravil Garifulin, Maksim Shmarov, Denis Logunov, et al.prepared using the NEBNext Ultra II Directional RNA Library Prep Kit and sample purification beads (NEB, #E7765S; New England Biolabs). Fragment library quality was assessed on the 2100 Bioanalyzer using a DNA High

(18) Epigenomic landscapes during prefrontal cortex development and aging in rhesusNational Science ReviewJuly 12, 2024Chao Ning, Xi Wu, Xudong Zhao, Zongyang Lu, Xuelong Yao, Tao Zhou, Lizhi Yi, Yaoyu Sun, Shuaishuai Wu, Zhenbo Liu, et al.libraries were prepared by using NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB, E7765s). The libraries were sequenced on Hiseq X10 with paired-end 150 bp (Illumina). Knockout of target gene

(19) Physiological Roles of an Acinetobacter-specific σ FactorbioRxivJuly 12, 2024Emily E. Bacon, Kevin S. Myers, Rubén Iruegas-López, Amy B. Banta, Michael Place, Ingo Ebersberger, Jason M. Petersprepared for next-generation sequencing using NEBNext Ultra II Directional RNA Library Prep kit (NEB; E7765S) and NEBNext Multiplex Oligos for Illumina (NEB; E6640S). Libraries were sequenced on Illumina NovaSeq

(20) The disease-causing tau V337M mutation induces tau hypophosphorylation and perturbs axon morphology pathwaysbioRxivJune 11, 2024Gregory A. Mohl, Gary Dixon, Emily Marzette, Justin McKetney, Avi J. Samelson, Carlota Pereda Pereda Serras, Julianne Jin, Andrew Li, Steven C. Boggess, Danielle L. Swaney, et al.No. E7405L). cDNA synthesis was then performed on all remaining RNAs (New England BioLabs, Cat. No. E7765S). Sequencing was performed at the Chan Zuckerberg Biohub and the UCSF Center for Advanced Techonlogy. ATAC-seq

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NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads

NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads from New England Biolabs

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NEBNextUltraII Directional RNA Library Prep with Sample Purification Beads from New England Biolabs

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