Fig 1: Gln supplementation in 6-month-old 3×Tg-AD mice exhibited inhibitory effects on mild cognitive impairment (MCI) and the induction of oxidative stress and amyloid beta (Aß) accumulation in the plasma. (A) Dietary groups and experimental schedule. (B) Body weights and (C) food intake were measured weekly. (D) Discrimination index (DI) in the object recognition test (ORT) in wild-type (WT, n = 5), 3×Tg-AD (3×Tg, n = 3), and Gln-diet 3×Tg (3×Tg+Gln, n = 4) mice at 2, 4, and 6 months of age. (E) Change in DI values in the ORT in mice between the age of 2 and 6 months. (F) Reactive oxygen/nitrogen species (ROS/RNS) analysis in plasma. (G) Aß1–42 content in neuron-derived exosomes (NDEs) isolated from plasma. ELISA results of Aß1–42 were normalized to those for CD81. Bars represent the means ± SEM. * p < 0.05, *** p < 0.001 vs. WT, and # p < 0.05 vs. 3×Tg in 1-way ANOVA with Tukey’s multiple comparison tests.
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