Fig 1: HIF-1α regulated right ventricular function and pathological manifestations in MCT-induced PAH-like rats through modulation of CD36 and Parkin/PINK1 signaling pathways. A Measurement of RVSP and RVHI. B Representative HE staining of lung tissues and quantitative analysis of vessel wall thickness/diameter ratio. Scale bar = 100 μm. C Representative Masson’s trichrome staining of lung tissues and quantitative analysis of collagen deposition. Scale bar = 100 μm. D Representative Oil Red O staining of lung tissues and quantitative analysis of positive staining. Scale bar = 100 μm. E Representative immunohistochemistry images and statistical analysis of PCNA-positive staining in lung tissues. F Western blotting analysis of PCNA and HIF-1α protein levels. G Serum levels of FAS, ACC, and CD36. *P < 0.05 vs. Normal. #P < 0.05 vs. PAH. &P < 0.05 vs. PAH + 2ME. @P < 0.05 vs. PAH + Sal. n = 6. The statistical test was performed using one-way ANOVA, followed by Tukey’s post hoc test
Fig 2: HIF-1α was closely associated with the Parkin/PINK1 axis and fatty acid metabolism-related factors in PAH patients. A Serum levels of HIF-1α were measured by ELISA. B Serum levels of Parkin and PINK1 were measured by ELISA. C Serum levels of FAS, ACC, and CD36 were measured by ELISA. *P < 0.05 vs. Normal. The statistical test was performed using unpaired Student’s t-test. D Spearman's correlation analysis was performed to determine the correlation between HIF-1α, Parkin, PINK1, FAS, ACC, and CD36. *P < 0.05. ***P < 0.001. ****P < 0.0001
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