Fig 1: P. distasonis enhanced CD8+T cell activity by secreting CXCL9 from macrophages in vivo. (A) Flow cytometry determination of positive proportion of CXCR3 in CD8+T cells in mouse tumor tissue. *P < 0.05 vs. PBS. (B) Tumor images. (C) Tumor volume growth curve. (D) Tumor weight. (E) IHC staining of Ki67 expression in mouse tumor tissue. Scale bar = 100 μm (100×), Scale bar = 25 μm (400×). (F and G) Flow cytometry determination of positive proportion of GZMB and IFN-γ in CD8+T cells in mouse tumor tissue. *P < 0.05 vs. αPD-1, &P < 0.05 vs. αPD-1 + P. distasonis
Fig 2: P. distasonis enhanced TAM-mediated CD8+T cell activity. (A and B) Flow cytometry analysis of positive proportion of GZMB and IFN-γ in CD8+T cells in mouse tumor tissue. *P < 0.05 vs. PBS. (C and D) Flow cytometry determination of proportion of GZMB+T and IFN-γ+T cells in primary CD8+T cells of spleen. (E) ELISA detection of secretion of IFN-γ, TNF-α, Perforin and GZMB in supernatant of primary CD8+T cells of spleen. *P < 0.05 vs. Control
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