Fig 1: a The number of mice in normal, irregular, and abnormal estrus cycle in each group; b The number of primordial, preantral and antral follicles and tatal number in each group; c The level of FSH, AMH and E2 in serum in each group.*P < 0.05 **P < 0.01, compared with the MOD group
Fig 2: Effect of ß-sitosterol on the expression of endometrium receptivity markers and related hormones in PCOS-like mice. (A) Expressions of COX-2, HOXA10, LIF, and Integrin a?ß3 in the endometrium of mice in each group were detected by western blot. (B) Levels of FSH, P, LH, and T in the serum of mice in each group were detected by ELISA. Data are presented as mean ± SEM. *P < 0.05 vs. Sham. #P < 0.05 vs. PCOS. FSH, follicle-stimulating hormone; P, progesterone; LH, luteinizing hormone; T, testosterone; PCOS, polycystic ovary syndrome.
Fig 3: Effect of ß-sitosterol-FMT on the expressions of endometrium receptivity markers and related hormones in mice with PCOS. (A) Expressions of COX-2, HOXA10, LIF, and Integrin a?ß3 in the endometrium of mice in each group were detected by western blot. (B) Levels of FSH, P, LH, and T in the serum of mice in each group were detected by ELISA. Data are presented as mean ± SEM. *P < 0.05 vs. Sham. #P < 0.05 vs. PCOS. PCOS, polycystic ovary syndrome; COX-2, cyclooxygenase-2; HOXA10, homeobox A10; LIF, leukemia inhibitory factor; ELISA, enzyme-linked immunosorbent assay.
Fig 4: Effect of model and treatment on sex hormone levels and mRNA expression in mice. Sex hormone levels of E2 (A), AMH (B), FSH (C), and LH (D). mRNA expression levels of FOXL2 (E), GDF9 (F), LIF (G), OCT4 (H), and SCF (I) in mouse ovarian tissues, as determined by real-time PCR. * p < 0.05, ** p < 0.01, *** p < 0.005.
Fig 5: Immunohistochemical and hormonal assessment analysis of testes from control mice and hyponatremic mice. A Immunohistochemical analysis of PCNA. In the scatter plot, densitometric analysis of positive pixels of PCNA positive cells is represented (**p = 0.02 group A and B vs. control group; ##p = 0.02, group A vs. B). Results are expressed as mean ± SD. B Immunohistochemical analysis of PTMA. In the scatter plot, densitometric analysis of positive pixels of PTMA-positive cells is represented (**p = 0.02 group A and B vs. control group; ##p = 0.02, group A vs. B). Results are expressed as mean ± SD. C Hormonal evaluation of testosterone (nmol/L), LH (UI/L) and FSH (UI/L) from mice with [Na+] = 140 mEq/L (hyponatremic mice) normalized vs. mice from control group. Results are expressed as mean ± SD (*p = 0.05). For immunohistochemical images: [Na+] 154 mEq/L is from control group; [Na+] 140 and 122 mEq/L are from group A, T3 and T8, respectively [Na+] 117 and 108 mEq/L are from group B, T8 and T14, respectively
Supplier Page from CUSABIO Technology LLC for Mouse follicle-stimulating hormone,FSH ELISA Kit